Comparative analysis of nuclear proteins of B cells in different developmental stages

Sun, Jing; Cho, Eun Hye; Ko, Jeong Hoon; Jung, Eun Hee; Ahn, Byungchan; Hahm, Jong Ryeal; Kim, Jae Won; Kim, Choong Won; Kim, Deok Ryong

doi:10.1002/pmic.200300576

Cited by 13 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For protein identification, we used a Voyager -DE™ (delayed extraction) STR biospectrometry workstation (Applied Biosystems, Forster city, CA, U.S.A.) for MALDI-TOF mass spectrometry. The gel pieces containing the desired protein spots were excised, washed and digested in gel with trypsin (sequencing grade, Boehringer Mannheim) (11). The resulting peptides were then processed for MALDI-TOF/MS.…”

Section: Methodsmentioning

confidence: 99%

Identification of Proteins Differentially Expressed in the Conventional Renal Cell Carcinoma by Proteomic Analysis

et al. 2005

Self Cite

View full text Add to dashboard Cite

Renal cell carcinoma (RCC) is one of the most malignant tumors in urology, and due to its insidious onset patients frequently have advanced disease at the time of clinical presentation. Thus, early detection is crucial in management of RCC. To identify tumor specific proteins of RCC, we employed proteomic analysis. We prepared proteins from conventional RCC and the corresponding normal kidney tissues from seven patients with conventional RCC. The expression of proteins was determined by silver stain after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The overall protein expression patterns in the RCC and the normal kidney tissues were quite similar except some areas. Of 66 differentially expressed protein spots (p<0.05 by Student t-test), 8 different proteins from 11 spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p<0.05); aminoacylase-1, enoyl-CoA hydratase, aldehyde reductase, tropomyosin α-4 chain, agmatinase and ketohexokinase. Two proteins, vimentin and α-1 antitrypsin precursor, were dominantly expressed in RCC (p<0.05).

show abstract

Section: Methodsmentioning

confidence: 99%

Identification of Proteins Differentially Expressed in the Conventional Renal Cell Carcinoma by Proteomic Analysis

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…Protein spots were isolated from the gel and identified by peptide mass fingerprinting using MALDI-TOF mass spectrometry as described previously (8, 9). …”

Section: Figmentioning

confidence: 99%

Disease-specific Proteins from Rheumatoid Arthritis Patients

et al. 2006

Self Cite

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is a chronic inflammatiory disease that mainly destroys cartilages or bones at the joints. This inflammatory disorder is initiated by self-attack using own immune system, but the detail of pathological mechanism is unclear. Features of autoantigens leading to autoimmune disease are also under veil although several candidates including type II collagen have been suggested to play a role in pathogenesis. In this report, we tried to identify proteins responding to antibodies purified from RA patients and screen proteins up-regulated or down-regulated in RA using proteomic approach. Fibronectin, semaphorin 7A precursor, growth factor binding protein 7 (GRB7), and immunoglobulin µ chain were specifically associated with antibodies isolated from RA synovial fluids. In addition, some metabolic proteins such as adipocyte fatty acid binding protein, galectin-1 and apolipoprotein A1 precursor were overexpressed in RA synovium. Also, expression of peroxiredoxin 2 was up-regulated in RA. On the contrary, expression of vimentin was severely suppressed in RA synoviocytes. Such findings might give some insights into understanding of pathological mechanism in RA.

show abstract

“…Proteomic approaches using two-dimensional gel electrophoresis allow one to identify proteins differentially expressed at each B lymphocyte developmental stage. According to the previous study [22], not only some proteins known as B cell lineage-specific factors such as E2A and LCK, but also some other proteins have been newly identified. Effort to isolate and identify proteins differentially expressed in the different stage during the B cell development is undergoing.…”

Section: Discussionmentioning

confidence: 98%