Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells

Abstract

To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which affect similar types of cells in the opposite way. Therefore, it is important to study and compare methods of vesicle isolation. Moreover, the differences between the EVs derived from tumor and mesenchymal stem cells are still poorly understood. This article compares EVs from human glioblastoma cells and mesenchymal stem cells (MSCs) obtained by two different methods, ultracentrifugation and cytochalasin B-mediated induction. The size of the vesicles, the presence of the main EV markers, the presence of nuclear and mitochondrial components, and the molecular composition of the vesicles were determined. It has been shown that EVs obtained by both ultracentrifugation and cytochalasin B treatment have similar features, contain particles of endogenous and membrane origin and can interact with monolayer cultures of tumor cells.

Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration

“… 21 Because CMVs can maintain the functional activity of surface receptors and cytosolic proteins derived from their parent cells, they have been widely investigated in cellular signaling and communication in recent years. 22 , 23 However, the effects and underlying mechanisms by which the combination of BMSC-derived CMVs and EC-derived CMVs promote bone regeneration are still largely unknown. Here, we successfully prepared human bone marrow mesenchymal stem cells derived CMVs (BMSC-CMVs) and human umbilical vein endothelial cells derived CMVs (EC-CMVs), which contain biologically active molecules derived from their parental cells (Fig.…”