Comparative analysis of nasal and oral mucosa dendritic cells

Allam, Jean‐Pierre; Niederhagen, Bernd; Bücheler, M.; Appel, T.; Betten, H.; Bieber, Thomas; Bergé, Stefaan; Novak, Natalija

doi:10.1111/j.1398-9995.2005.00965.x

Cited by 77 publications

(66 citation statements)

References 23 publications

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“…The importance of Langerhans cells in the induction of immune tolerance to allergens has been highlighted in mechanistic studies of SLIT. Oral Langerhans cells constitutively express the high-affinity receptor for IgE (FceRI) and Fcg receptors, allowing them to bind allergen, and are the main dendritic cell subset in the oral mucosa (28). These cells display an immature phenotype.…”

Section: Route Of Administration and Modulation Of Immunitymentioning

confidence: 99%

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Larché

2014

Annals ATS

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Allergen immunotherapy with whole proteins is clinically efficacious but requires a protracted treatment period because of frequent allergic adverse events. A combination of duration of treatment and adverse events leads to poor compliance. Short synthetic peptides containing the major immunodominant T cell epitopes of allergenic proteins have been shown to reduce IgE cross-linking ability, thereby leading to fewer allergic adverse events following their administration to patients with allergies. Peptide immunotherapy has been shown to result in clinically meaningful efficacy in several Phase II, randomized, double-blind, placebo-controlled clinical trials. Exactly how peptide immunotherapy achieves its efficacy remains incompletely understood, but the mechanisms are thought to include immune deviation and induction of regulatory T cells capable of suppressing allergen-specific immune responses. Limited data are available on the effects of peptide therapy on humoral immune responses. Induction of allergen-specific IgG has been observed after peptide therapy, but the levels of antibody induced were much lower than generally seen with the utilization of whole allergen approaches. Thus, the immunological mechanisms of peptide immunotherapy appear to overlap, although not completely, with those seen in whole allergen therapy. Further studies are required to fully elucidate mechanisms of action.

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Section: Route Of Administration and Modulation Of Immunitymentioning

confidence: 99%

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Larché

2014

Annals ATS

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“…Immature Langerhans-type dendritic cells (iLC) are the only professional antigen-presenting cells located in the outermost layers of the oral mucosa (9)(10)(11)(12)(13)(14). As such, iLC are bound to be the first immune cells to encounter invading pathogens that access their hosts via the oral cavity, such as CMV.…”

mentioning

confidence: 99%

Dynamics of Human Cytomegalovirus Infection in CD34⁺Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells

Coronel¹,

Takayama²,

Juwono³

et al. 2015

J Virol

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Acquisition of human cytomegalovirus (CMV) usually occurs by contact between contaminated bodily fluids, such as urine and saliva, and host mucosal cells. Langerhans-type dendritic cells (LC) are the only type of immune cells found in the outermost layers of the oral mucosae, where they not only provide a first line of defense against CMV but can easily be targeted by orally administered vaccines, while their bone marrow resident progenitors are important sites of virus latency. In this work, we tracked the progress of infection in CD34 ؉ progenitor cells, immature LC (iLC), and mature LC (mLC) exposed to the clinicallike strain TB40-BAC4 or to the vaccine strain AD169varATCC, prior to their long-term maintenance under either immature or mature conditions. We show that the genomes of both strains are efficiently maintained in CD34؉ cells during their differentiation into iLC, although this requires the presence of larger amounts of input AD169varATCC DNA. Lipopolysaccharide-and CD40 ligand-induced maturation of iLC derived from latently infected progenitors was not associated with robust viral genome replication and progeny production, while maturation of directly infected iLC increased and prolonged expression of the viral immediate early proteins. While effective replication of viral genomes from both strains occurred only in mLC, both iLC and mLC produced viral progeny, suggesting that both types of LC may contribute to CMV horizontal transmission in vivo. H uman cytomegalovirus (CMV), a major cause of disease and death in immunocompromised and in congenitally infected individuals, is normally acquired by contact between infected bodily fluids such as urine and saliva and host mucosal surfaces, particularly those lining the oral and nasal cavities. IMPORTANCE Human CMV is usually acquired via the oral and nasal mucosae. Langerhans-type dendritic cells (LC) are the only type of immune cells found in the outermost layers of these tissues. Understanding how CMV interacts with LC and their hematopoietic progenitors is thus essential to develop innovative means of defense against this virus. Here we show that the genomes of a viru-From these peripheral sites, CMV is thought to reach the circulation and hence the bone marrow, where latency is established in myeloid cells, including CD34 ϩ hematopoietic progenitors (1-5). Reactivation from latency in the myeloid progeny of these cells is then believed to be the source of newly produced viral particles, which upon amplification in oral epithelial cells are released in the saliva and contribute to CMV horizontal transmission in vivo (6-8).Immature Langerhans-type dendritic cells (iLC) are the only professional antigen-presenting cells located in the outermost layers of the oral mucosa (9-14). As such, iLC are bound to be the first immune cells to encounter invading pathogens that access their hosts via the oral cavity, such as CMV. Upon contact with "danger" signals, iLC migrate toward the draining lymph nodes and begin the process of maturation, which culmina...

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“…Существует предположение, что дендритные клетки слизистой ротовой полости, подобные клеткам Лангерганса, играют решающую роль при сублингвальном введении аллергенов [8,13]. Во время сублингвальной АСИТ аллерген захва-тывается этими клетками, которые созревают и мигрируют в регионарные лимфатические узлы.…”

Section: гайдук им и др медицинская иммунологияunclassified

Changes of Immune Indexes During Sublingual Allergen-Specific Immunotherapy in Children With Hay Fever

Гайдук¹,

Коростовцев²,

Шапорова³

et al. 2014

Med.Imm.Rus

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Резюме. Цель исследования: оценить динамику иммунологических показателей при проведе-нии сублингвальной аллерген-специфической иммунотерапии смесью из пыльцы деревьев у детей с поллинозом. Материалы и методы: в исследование включено 100 больных в возрасте от 5 до 18 лет, страдающих поллинозом. Аллерген-специфическую иммунотерапию проводили в предсезонный пе-риод в течение 3 лет. Динамику цитокинов в сыворотке крови и в смывах из полости носа изучали до лечения и после 2-го и 3-го курса лечения. Результаты: в процессе лечения выявлено повышение концентрации IL-10, IFNγ и уменьшение содержания IL-4 в сыворотке крови. Значимой динамики содержания цитокинов в смывах из полости носа не выявлено. Выводы: выявленные изменения со-ответствуют изменению профиля Т-клеточного ответа в сторону Th1-пути, что подтверждает эффек-тивность сублингвальной аллерген-специфической иммунотерапии на патогенетическом уровне. Changes of immune indexes during sublingual allergen-speCifiC immunotherapy in Children with hay feverabstract. Aims of study: evaluation of immunological parameters in course of sublingual allergen-specific immunotherapy with tree pollen mixture in children with hay fever. Materials and methods: the study included one-hundred patients 5 to 18 years of age with hay fever (pollen rhinitis, rhinoconjunctivitis and/or asthma). Allergen-specific immunotherapy was administered pre-seasonally for three consecutive years. Cytokine changes were studied in blood serum and in lavages from nasal cavity. Samples assessed before treatment and after 2 nd and 3 rd courses SLIT completion. Results: increased serum concentrations of IL-10, IFNγ, and decreased IL-4 contents were revealed in the course of treatment. No significant changes in cytokine concentrations were detectable in nasal lavages. Conclusions: the changes revealed correspond to a shift of T cell response profile towards Th1 pathway, thus confirming pathogenetic effects of sublingual allergen-specific therapy.

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Comparative analysis of nasal and oral mucosa dendritic cells

Cited by 77 publications

References 23 publications

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Dynamics of Human Cytomegalovirus Infection in CD34⁺Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells

Changes of Immune Indexes During Sublingual Allergen-Specific Immunotherapy in Children With Hay Fever

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Comparative analysis of nasal and oral mucosa dendritic cells

Cited by 77 publications

References 23 publications

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Mechanisms of Peptide Immunotherapy in Allergic Airways Disease

Dynamics of Human Cytomegalovirus Infection in CD34+Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells

Changes of Immune Indexes During Sublingual Allergen-Specific Immunotherapy in Children With Hay Fever

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Dynamics of Human Cytomegalovirus Infection in CD34⁺Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells