Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Ghimire, Subash; Jeught, Margot Van der; Neupane, Jitesh; Roost, Matthias S; Anckaert, Jasper; Popovic, Mina; Nieuwerburgh, Filip Van; Mestdagh, Pieter; Vandesompele, Jo; Deforce, Dieter; Menten, Björn; Lopes, Susana Chuva de Sousa; Sutter, Petra De; Heindryckx, Björn

doi:10.1038/s41598-018-24051-5

Cited by 61 publications

(62 citation statements)

References 23 publications

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“…Compared to ESCs, we found that the majority of pluripotency markers such as Rex1, Klf4, Sox2 and Nanog were downregulated in EPI aggregates, but Oct4 expression was at similar levels. Furthermore, the expression of the epiblastspecific markers Fgf5 and Otx2 was highly upregulated in EPI aggregates, indicating a transition from the naïve to the primed state of pluripotency (Ghimire et al 2018) (Fig. 1d).…”

Section: High-throughput Formation Of Epi Aggregates In Hydrogel Micrmentioning

confidence: 96%

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Girgin

2020

Preprint

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When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and patterning along the three major body axes. However, these structures, called gastruloids, currently lack the anterior embryonic regions, such as those belonging to the brain. Here we describe an approach to generate gastruloids that have a more complete antero-posterior development. We used bioengineered hydrogel microwell arrays to promote the robust derivation of mouse ESCs into post-implantation epiblast-like (EPI) aggregates in a reproducible and scalable manner. These EPI aggregates break symmetry and axially elongate without external chemical stimulation. Inhibition of WNT signaling in early stages of development leads to the formation of gastruloids with anterior neural tissues. Thus, we provide a new tool to study the development of the mouse after implantation in vitro, especially the formation of anterior neural regions.

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Section: High-throughput Formation Of Epi Aggregates In Hydrogel Micrmentioning

confidence: 96%

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Girgin

2020

Preprint

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“…The loss of Dppa3 has no obvious effects on either germ cell determination. 26 Dppa3-positive ES cells have more active histone modification and lower DNA methylation. Dppa3-deficient females failed to produce offspring.…”

Section: Maintenance Of the Pluripotencymentioning

confidence: 99%

Dppa3 in pluripotency maintenance of ES cells and early embryogenesis

Zhao

Liu

et al. 2018

J of Cellular Biochemistry

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Embryonic development is precisely regulated by a network of signal pathways and specific genes. Dppa3 (also known as Pgc7 or Stella) plays an important role in early embryonic development during the cleavage stage as a maternal effect gene. Dppa3 expresses in many species, and its homologous gene in human and rat genomes is located at the same chromosomal regions and have the same exon‐intron structure. However, unlike mouse embryonic stem (ES) cells, in which the Dppa3 promoter maintains hypomethylation that allows a high transcription level, the DPPA3 promoter region in human ES cells is methylated, much like that of mouse epiblast stem cell. Dppa3 is essential for early embryogenesis and pluripotency maintenance; however, the precise mechanism and downstream passage remains unknown. In this review, we will summarize some important functions of Dppa3 in early embryogenesis and pluripotency maintenance of mouse ES cells.

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“…The detection of these markers is usually performed using flow cytometry, RT-qPCR, and cell-staining techniques 100 . A number of markers can be used to identify PSC types, such as the naive PSC state or the high-yield expansion PSC state [101][102][103][104] , and to measure the quality of the PSCs 105 . At the single-cell level, pluripotency can be assessed with scRNA-seq and bioinformatics tools.…”

Section: Cell Type Authentication For Regenerative Medicinementioning

confidence: 99%

Human Cell Atlas and cell-type authentication for regenerative medicine

et al. 2020

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In modern biology, the correct identification of cell types is required for the developmental study of tissues and organs and the production of functional cells for cell therapies and disease modeling. For decades, cell types have been defined on the basis of morphological and physiological markers and, more recently, immunological markers and molecular properties. Recent advances in single-cell RNA sequencing have opened new doors for the characterization of cells at the individual and spatiotemporal levels on the basis of their RNA profiles, vastly transforming our understanding of cell types. The objective of this review is to survey the current progress in the field of cell-type identification, starting with the Human Cell Atlas project, which aims to sequence every cell in the human body, to molecular marker databases for individual cell types and other sources that address cell-type identification for regenerative medicine based on cell data guidelines.

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Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background

Cited by 61 publications

References 23 publications

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

Dppa3 in pluripotency maintenance of ES cells and early embryogenesis

Human Cell Atlas and cell-type authentication for regenerative medicine

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