Comparative analysis of microRNA expression profiles between A549, A549/DDP and their respective exosomes

Abstract

Exosomes were reported to transport bioactive molecules and influence the biology behavior of recipient cells. In order to study the role of exosomal microRNAs in the mechanism of cisplatin resistance to lung cancer cells, we analyzed the expression profiles of microRNAs in A549, A549/DDP cells and their exosomes by microarray. The results showed that a certain proportion of microRNAs were co-expressed in the cells and exosomes. Linear regression analysis showed that the expression of microRNAs in A549 and A549/DDP cells were strongly correlated with those in their respective exosomes. The expression level of 5 microRNAs (miR-197-5p, miR-4443, miR-642a-3p, miR-27b-3p and miR-100-5p) with the most differential expression were verified by qRT-PCR. The results were consistent with those of the microarray. Target gene prediction and pathway analysis discovered that the microRNAs in the intersections may participate in drug resistance. And the prediction of their association with diseases found that most of these microRNAs was associated with lung cancer. We could draw a preliminary conclusion that microRNAs in exosomes may be involved in the drug resistance of lung cancer cells to cisplatin.

Cisplatin-resistant lung cancer cell–derived exosomes increase cisplatin resistance of recipient cells in exosomal miR-100–5p-dependent manner

“…As in our previous work, 21 significant correlation between the miRNA profiles of A549 cells and their exosomes was detected by linear regression analysis (Figure 2A). The miRNA profiles of A549/DDP cells and exosomes showed similar results ( Figure 2B).…”

“…rNa exaction from cells and exosomes for microarray analysis As in our previous work, 21 miRNA expression profiles of lung cancer cells A549 and its DDP-resistant cells A549/ DDP were analyzed by microarray. MiRNA expression profiles of exosomes derived from A549 and A549/DDP were also analyzed by microarray.…”

Glucagon-Like Peptide-1 (GLP-1) Receptor Agonist Liraglutide Alters Bone Marrow Exosome-Mediated miRNA Signal Pathways in Ovariectomized Rats with Type 2 Diabetes

“…Exosomal miRNA expression profiles can be altered by different diseases and drug administration [ 26 , 27 ]. Variance in miRNA expression profiles is involved in disease progression, drug action, and drug resistance [ 14 , 15 ].…”

MicroRNAs: A novel potential biomarker for diagnosis and therapy in patients with non‐small cell lung cancer

“…14,15,126 Several lines of studies have identified that exosomal miRNA performed different expression profiles between lung cancer patients and controls, suggesting exosomal miRNA might be as a novel screening modality for patients with lung cancer. [127][128][129][130] Thus, it has opened the window to a new world of possibilities to diagnose NSCLC in early stage. naive MSCs into a novel kind of pro-inflammatory MSCs (P-MSCs) by activating TLR2/NF-kB signalling through exosomal surface HSP70.…”