Comparative Analysis of Mechanical and Bioprosthetic Valves Following Aortic Valve Replacement

Borkon, A. Michael; Soule, Lisa; Baughman, Kenneth L.; Baumgardner, W. A.; Gardner, Timothy J.; Watkins, Levi; Gott, Vincent L.; Reitz, Bruce A.

doi:10.1007/978-1-4684-6900-4_23

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Courtman in (1995 indicated that a tissue valve for homograft or xenograft transplant has several advantages such as freedom from coagulation, low incidence of thromboembolism and relative resistance to endocarditis (Courtman et al 1995), however it could be possible that they have less durability than mechanical valves (Borkon et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

Vázquez

Roman

et al. 2008

Cell Tissue Banking

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To analyse the influence of cold ischemic time (CIT) (2-24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (-1 degrees C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis.

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Section: Discussionmentioning

confidence: 99%

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

Vázquez

Roman

et al. 2008

Cell Tissue Banking

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“…At the beginning of the aortic valve replacement history, the homograft valve was used following simple storage in a cool solution (3). Shortly afterward, a renovated mechanical valve replaced the use of the homograft valve (4). A cryopreservation technique changed the standpoint of the homograft valve (5).…”

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Short‐Term Result of Aortic Valve Replacement with Cryopreserved Homograft Valve in the University of Tokyo Tissue Bank

et al. 2002

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The short-term results after aortic root replacement with 11 cryopreserved aortic homografts was examined. Since 1998, the University of Tokyo Tissue Bank has supplied 11 aortic homograft valves. Nine of the recipients were male, and the average age was 51.2 years. Nine out of 11 patients had suffered from a serious condition of native or prosthetic valve infectious endocarditis. All of the patients underwent aortic root replacement, and the blood type between the patient and the homograft was matched in 8 of the patients. Only 1 patient died (9.1%) in the short-term due to sepsis. The preoperative degree of aortic valve regurgitation in all of the cases was third or fourth while the regurgitation disappeared after the operation in all of them. Thinking of the serious condition of our cases preoperatively, the 9.1% operative mortality was quite acceptable. Long-term follow-up is necessary to estimate the quality of the homografts.

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Comparative Analysis of Mechanical and Bioprosthetic Valves Following Aortic Valve Replacement

Cited by 2 publications

References 32 publications

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

Short‐Term Result of Aortic Valve Replacement with Cryopreserved Homograft Valve in the University of Tokyo Tissue Bank

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