Comparative Analysis of Long Noncoding RNAs Expressed during Intramuscular Adipocytes Adipogenesis in Fat-Type and Lean-Type Pigs

Abstract

The meat quality of local breed pigs is more tender and juicier than the imported varieties. The important reason is that the intramuscular fat content is high. Even through modest sequence conservation and evolution, the expression pattern and function of long noncoding RNAs (lncRNAs) seem to be conserved. In spite of that, analysis of lncRNAs associated with intramuscular fat development remains unknown to us in porcine. Here, we systematically investigated lncRNAs of intramuscular adipocytes of fat local Bamei pigs and lean Large White pigs to consider the function of lncRNAs on intramuscular fat development. We selected three piglets of both breeds separately to isolate intramuscular preadipocytes, performed RNA sequencing across four stages (0, 2, 4, and 8 d) during the intramuscular preadipocytes differentiation, and identified 1932 lncRNAs (760 novel). In addition, we have screened lnc_000414 closely related to fat synthesis. This lncRNA function as an inhibitor in the proliferation of porcine intramuscular adipocytes. These novel findings will provide new targets for improving pork quality and making pig breeding better.

Screening of lncRNA Profiles During Intramuscular Adipogenic Differentiation Inlongissimus Dorsi and Semitendinosus Muscles in Pigs

“…With regards to intramuscular fat accumulation, adipocyte proliferation and differentiation are two important factors that contribute to lipid deposition [27]. In previous studies, lncRNAs were found to exert a regulatory role in the proliferation of porcine intramuscular adipocytes [9]. Our study showed that lncRNA may also modulate the adipogenic process of intramuscular adipocytes.…”

“…Moreover, PU.1 AS lncRNA has also been found to promote adipogenic differentiation in porcine intramuscular adipocytes [8]. Furthermore, lncRNAs were differentially expressed in pig breeds with differing IMF contents [9]. These studies indicate that lncRNAs may be critical modulators in porcine IMF accumulation, thus potentially providing novel breeding selection markers that select for improved pork quality.…”

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

“…AQ pigs, one Chinese fat-type pig breed, have high good meat quality and slow growth rate [23]. Whereas the Chinese domestic breed has the universal characteristics of higher fat deposition capacity compared to that with Western commercial pig breeds [24]. Accordingly, the two pig breeds can be taken as ideal animal models to comparatively study the mechanisms controlling adipogenesis.…”

IRLnc: A novel functional noncoding RNA contributes to intramuscular fat deposition

“…Although several lincRNAs have been reported associated with pork and poultry IMF [12][13][14][15], little is known about the mechanism of lincRNA gene regulation in pig IMF.. The objectives of this work were to perform RNA sequencing analysis using an IMF character segregation population which construct using high IMF pigs (Min pig) and low IMF pigs (Large white pigs) crossbred and to analyze the joint or independent effects of lincRNAs on IMF.…”