Comparative analysis of insect gut symbionts for composition–function relationships and biofuel application potential

Shi, Weibing; Uzuner, Uğur; Huang, Lingxia; Jesudhasan, Palmy R.; Pillai, Suresh D.; Yuan, Joshua S.

doi:10.4155/bfs.11.127

Cited by 6 publications

(4 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some microorganisms that seem related to PVC biodegradation include the bacterial strains Mycobacterium sp. NK0301 ( Nakamiya et al, 2005 ); Chryseomicrobium imtechense, Lysinibacillus fusiformis, Acinetobacter calcoaceticus, Stenotrophomonas pavanii ( Latorre et al, 2012 ), Acanthopleurobacter pedis, Bacillus cereus, Bacillus aerius ( Shi et al, 2011 ; Anwar et al, 2016 ), Bacillus flexus ( Giacomucci et al, 2019 ), Bacillus sp. AIIW2 ( Kumari et al, 2019 ), Pseudomonas otitidis ( Shi et al, 2011 ; Anwar et al, 2016 ), P. aeruginosa, P. putida, Pseudomonas citronellolis ( Shi et al, 2011 ; Giacomucci et al, 2019 ), Microbacterium sp.…”

Section: Microbial Depolymerization Of Plastics Trough Pathways For D...mentioning

confidence: 99%

“…AIIW2 ( Kumari et al, 2019 ), Pseudomonas otitidis ( Shi et al, 2011 ; Anwar et al, 2016 ), P. aeruginosa, P. putida, Pseudomonas citronellolis ( Shi et al, 2011 ; Giacomucci et al, 2019 ), Microbacterium sp. and Bacterium Te68R ( Shi et al, 2011 ). PVC degradation was accomplished in some extent by the fungal strains Alternaria sp.…”

Section: Microbial Depolymerization Of Plastics Trough Pathways For D...mentioning

confidence: 99%

“…Selected throughout insects and microbes’ evolution, these associations may teach many lessons on how to find efficient microbial degraders and detoxifiers of plant tissues, how to assemble an efficient plant-degrading community, and how to promote metabolic interactions for obtaining nutrients from recalcitrant polymers. Investigating the microbial strategies for decaying plant biomass could bioinspire the tuned application of hydrolytic and oxidative pathways to degrade plastics and to generate value-added products ( Holladay et al, 2007 ; Cook and Doran-Peterson, 2010 ; Huang et al, 2010 ; Sun and Scharf, 2010 ; Shi et al, 2011 ; Koch et al, 2014 ; Wang et al, 2015 ; Dangles and Casas, 2019 ; Tiso et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

Barcoto

Rodrigues

2022

Front. Microbiol.

View full text Add to dashboard Cite

Anthropogenic activities have extensively transformed the biosphere by extracting and disposing of resources, crossing boundaries of planetary threat while causing a global crisis of waste overload. Despite fundamental differences regarding structure and recalcitrance, lignocellulose and plastic polymers share physical-chemical properties to some extent, that include carbon skeletons with similar chemical bonds, hydrophobic properties, amorphous and crystalline regions. Microbial strategies for metabolizing recalcitrant polymers have been selected and optimized through evolution, thus understanding natural processes for lignocellulose modification could aid the challenge of dealing with the recalcitrant human-made polymers spread worldwide. We propose to look for inspiration in the charismatic fungal-growing insects to understand multipartite degradation of plant polymers. Independently evolved in diverse insect lineages, fungiculture embraces passive or active fungal cultivation for food, protection, and structural purposes. We consider there is much to learn from these symbioses, in special from the community-level degradation of recalcitrant biomass and defensive metabolites. Microbial plant-degrading systems at the core of insect fungicultures could be promising candidates for degrading synthetic plastics. Here, we first compare the degradation of lignocellulose and plastic polymers, with emphasis in the overlapping microbial players and enzymatic activities between these processes. Second, we review the literature on diverse insect fungiculture systems, focusing on features that, while supporting insects’ ecology and evolution, could also be applied in biotechnological processes. Third, taking lessons from these microbial communities, we suggest multidisciplinary strategies to identify microbial degraders, degrading enzymes and pathways, as well as microbial interactions and interdependencies. Spanning from multiomics to spectroscopy, microscopy, stable isotopes probing, enrichment microcosmos, and synthetic communities, these strategies would allow for a systemic understanding of the fungiculture ecology, driving to application possibilities. Detailing how the metabolic landscape is entangled to achieve ecological success could inspire sustainable efforts for mitigating the current environmental crisis.

show abstract

Section: Microbial Depolymerization Of Plastics Trough Pathways For D...mentioning

confidence: 99%

Section: Microbial Depolymerization Of Plastics Trough Pathways For D...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

Barcoto

Rodrigues

2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Dillon and Charnley studied the numbers and types of intestinal microflora in Schistocerca gregaria and demonstrated how different diets influenced gut microbe numbers and varieties ( Dillon and Charnley 2002 ). Shi et al studied the microbial community structures of gut symbionts in woodbore, silkworm, grasshopper and cutworm and observed significant differences in symbiotic community structure correlated with food adaptation ( Shi et al 2011 ). However, because traditional sequencing technology is low-throughput and time-consuming, the exploration of insect gut bacterial diversity has been limited.…”

Section: Introductionmentioning

confidence: 99%

The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding

Liu¹,

Zhao²,

Qiu³

et al. 2023

BDJ

View full text Add to dashboard Cite

Most orthopteran insects are phytophagous and some are important pests in agriculture and forests. Many intestinal microflora of Orthoptera insects have been reported, primarily from Acridoidea and there have been few reports of other taxa. In this study, we collected 15 individuals representing five species (Ruspolia lineosa, Tetrix japonica, Erianthus versicolor, Gryllotalpa orientalis and Teleogryllus emma) belonging to five orthopteran superfamilies (Tettigonioidea, Tetrigoidea, Eumastacoidea, Gryllotalpoidea and Grylloidea) to characterise and compare the gut microbiota with greater taxonomic width by performing sequencing analysis of the 16S rRNA V4 region in gut material. A total of 606,053 high-quality sequences and 3,105 OTUs were acquired from 15 gut samples representing 24 phyla, 48 classes, 69 orders, 133 families and 219 genera. Firmicutes and bacteria were the most abundant phyla, followed by Bacteroidetes, Cyanobacteria, Actinobacteria and Acidobacteria. At the genus level, Serratia, Citrobacter, Wolbachia, Lactobacillus and Parabacteroides were the most predominant genera in R. lineosa, T. japonica, E. versicolor, G. orientalis and T. emma, respectively. Both Principal Coordinates Analysis (PCoA) and heatmap results revealed significant differences in bacterial community composition across species. Additionally, alpha diversity analysis indicated the bacterial richness was significantly different amongst the five species.

show abstract

Biofuels: Fungal, Bacterial and Insect Degraders of Lignocellulose

Sethi

Scharf

2013

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Lignocellulose is the main structural component of plant cell walls and can be degraded into simple sugars with the help of hydrolytic enzymes. Lignocellulose is composed of three polymers: cellulose, hemicellulose and lignin. Certain fungi, bacteria and insects have evolved the ability to degrade lignocellulose. Cellulose is degraded to glucose by the synergistic action of three distinct classes of enzymes: endoglucanases, exoglucanases and β‐glucosidases. Owing to its variable structure and organisation, hemicellulose degradation requires various enzymes with diverse modes of action, which include endoxylanases, endomannanases, β‐xylosidases, β‐mannosidases, β‐galactosidases, α‐glucuronidases, α‐arabinofurnosidases, α‐galactosidases, acetyl xylan esterases, feruloyl esterases and glucuronyl esterases. Lignin degradation is much more difficult due to its complex structure and bonding to carbohydrate complexes. It requires oxidative enzymes, such as lignin peroxidases, manganese peroxidases, versatile peroxidases, laccases, and several other auxiliary enzymes. Commercial biofuel production from lignocellulosic materials can be achieved by artificially producing these cellulases, hemicellulases and lignases in recombinant form. Key Concepts: Lignocellulose is the most abundant renewable resource of energy on the Earth. Lignocellulose is a general term referring to a natural complex of three biopolymers: cellulose, hemicellulose and lignin. Simple sugar can be released from lignocellulose using three categories of enzymes: cellulases, hemicellulase and lignases. Released sugars from lignocellulose can be fermented into biofuel (ethanol). Wood‐rotting fungi such as white‐rot, brown‐rot and soft‐rot fungi can degrade lignocellulose. Aerobic and anaerobic bacteria such as Bacillus, Streptomyces, Cellulomonas and Clostridium possess lignocellulose‐degrading enzymes. Wood and plant‐feeding insects such termites, roaches, longhorn beetles and grasshoppers are rich sources of novel lignocellulases genes. Lignocellulases of fungal, bacterial and insect origin can be artificially/recombinantly produced in various protein expression systems. Fungal, bacterial and insect lignocellulases are expected to play important roles in the development of successful lignocellulose conversion technologies.

show abstract

Comparative analysis of insect gut symbionts for composition–function relationships and biofuel application potential

Cited by 6 publications

References 77 publications

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

Lessons From Insect Fungiculture: From Microbial Ecology to Plastics Degradation

The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding

Biofuels: Fungal, Bacterial and Insect Degraders of Lignocellulose

Contact Info

Product

Resources

About