Comparative analysis of inductive agents in rat normal liver and ascites hepatoma

Kawakami, Izumi; Iyeiri, Sumiko

doi:10.1002/jez.1401490204

Cited by 5 publications

(2 citation statements)

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“…The experimental evidences, that ribonucleoprotein (9) and ribosomal fraction (1 3 ) induce neural tissue, and nearly noninductive rat ascites hepatoma becomes inductive by homogenization (12), testify the inductive capacity of some intracellular substances. However, as we will demonstrate in a following paper artificially produced histone-polyanion complexes show strong neural inductive effect, it may be that inductive capacity is not exclusive a property of certain specific proteins, and that proteins with inductive properties do not necessarily correspond to the genuine organizer substance or are not necessarily chemically similar to the organizer substance.…”

Section: Discussionmentioning

confidence: 99%

“…The type, however, is not always the same among those induced by homotypic tissues from different kinds of animals. For example, as contrast with almost purely neural or archenphalic induction by guinea-pig and rat liver (12,25), livers of newt and mouse are archencephalicdeuterencephalic inductor (1,2,4), showing mesoderm inducing capacity mixed with neural inductive one, though the former is weak.…”

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Difference in Inductive Effect of Liver Tissues With and Without Perisinusoidal Basement Membrane

1976

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Differential inductive capacities among liver tissues of several animals were examined by anticipating the correlation between the capacity and the completness of perisinusoidal basement membrane.The reacting tissue was competent ectoderm of gastrula of Triturus pyrrhogaster, and the inductive effects of livers on the ectoderm were tested by explantation method. The inductive effect of livers being devoid of the membrane (chick and guinea pig) was neural and the tissues having the dense well-developed membrane (reptiles) produced an assembly of neural and mesodermal tissues, such as notochord and somite or muscle. The livers with the membrane being of intermediate grade of development (calf, Triturus and mouse) induce mesodermal tissues, but not frequently, together with neural tissue or alone. The liver tissue was more active in mesodermal induction in proportion to the completeness of the perisinusoidal basement membrane.On the basis of these data the difference in inductive capacity among liver tissues from different kinds of animals were discussed.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Difference in Inductive Effect of Liver Tissues With and Without Perisinusoidal Basement Membrane

1976

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Isolation of mesoderm-inducing protein from chick embryos

Kawakami

Iyeiri

1964

Experimental Cell Research

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Inductive Effects of Nuclei and Their Subfractions Isolated From Rat Liver

Suzuki

Kawakami

1963

Embryologia

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It is well known that animal tissues have the capacity to induce tissue differentiation when they act upon amphibian presumptive epidermis. Recently, many papers have been published concerning separation of the inductive agents from heterogeneous tissues and their distribution in the cell (TIEDEMANN et al., 1956;YAMADA, 1958;KAWAKAMI et al., 1961). According to K~W A K A M I and IYEIRI (1962 a), the neural or archencephalic induction is produced by ribonucleoprotein particles, and the non.ribonucleoprotein fraction isolated from mesoderm-inducing tissues is responsible for the inducing effect of the tissues. Thus distribution of the inductive agents in cytoplasm has been demonstrated, but few papers have referred to the nucleus. This brief note will report data which have been obtained on the distribution of the inductive agents in isolated nuclei. MATERIALS AND METHODSRat liver nuclei were isolated by the method reported by RENDI (1960). and fractionated by the methods of ALLFREY et al. (1957) with some modification. The nuclei were extracted first with 0.1 M Tris-phosphate buffer (pH 7.1) and centrifuged down at 1,000 g for 10 minutes. The supernatant was termed "neutral buffer extract". The sediment was extracted again with 1 M NaCI, followed by centrifugation a t 26,000 g for 60 minutes, and the supernatant and resulting sediment were termed "1 M NaCl extract" and "residue", respectively. T h e neutral buffer extract was centrifuged a t 105,000 g for 90 minutes and the resulting sediment was termed "nuclear ribosome fraction". The inductive capacities of these materials were tested by on the presumptive epidermis of Triturw hrrhogaster gastrulae. Chemical analysis of RNA was performed by the orcinol method, and DNA was analysed by the diphenylamine method. The samples of isolated nuclei were examined under both light and electron microscopes. For electron microscopical observation, the nucleus samples were fixed by Veronal-buffered osmium tetroxide (pH 7.3, l,q), dehydrated and embedded in n-butyl methacrylate (PALADE, 1952 a). 75

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Comparative analysis of inductive agents in rat normal liver and ascites hepatoma

Cited by 5 publications

References 11 publications

Difference in Inductive Effect of Liver Tissues With and Without Perisinusoidal Basement Membrane

Difference in Inductive Effect of Liver Tissues With and Without Perisinusoidal Basement Membrane

Isolation of mesoderm-inducing protein from chick embryos

Inductive Effects of Nuclei and Their Subfractions Isolated From Rat Liver

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