Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters

Lantos, Csaba; Bóna, L.; Boda, Krisztina; Pauk, János

doi:10.1007/s10681-013-1031-y

Cited by 39 publications

(35 citation statements)

References 29 publications

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“…Besides spontaneous polyploidization, chromosome doubling may also be induced by in vitro or in vivo application of chromosome doubling agents such as colchicine (Melchinger et al 2016;Ślusarkiewicz-Jarzina et al 2017). In contrast to triticale (Lantos et al 2014) and wheat (Rubtsova et al 2013) however, this is not considered necessary in perennial ryegrass due to a high level of natural chromosome doubling (Andersen et al 1997).…”

Section: Robustness Of Androgenic Capacitymentioning

confidence: 99%

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

Begheyn

Roulund

Vangsgaard

et al. 2017

Plant Cell Tiss Organ Cult

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Section: Robustness Of Androgenic Capacitymentioning

confidence: 99%

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

Begheyn

Roulund

Vangsgaard

et al. 2017

Plant Cell Tiss Organ Cult

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“…However, homozygous, genetically uniform and stable doubled haploids (DHs) are necessary to maximize heterosis. Such forms could be derived via androgenesis employing anther cultures or isolated microspores (Eudes and Amundsen 2005;_ Zur et al 2008;Lantos et al 2013;Würschum et al 2013).…”

Section: Introductionmentioning

confidence: 99%

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Machczyńska

Orłowska

Mańkowski

et al. 2014

Plant Cell Tiss Organ Cult

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Doubled haploids of triticale are of interest for plant breeders due to hybrid breeding programs based on cytoplasmic male sterility Tt phenomenon. However, (epi)mutations appearing during in vitro culture regeneration may lead to a phenotypic variation that makes the uniformity of plant materials questionable. Using RP-HPLC genomic DNA methylation of donor doubled haploid plants utilized as a source of tissues for the in vitro regeneration (via androgenesis and somatic embryogenesis) of triticale cv. Bogo and their consecutive generative progeny was evaluated. It was demonstrated that in vitro cultures induced a decrease of the DNA methylation of the regenerants independently of the approach used for plant regeneration. The decrease in DNA methylation of genomic DNA proceeded up to the first/second successive generations followed by the beginning of its reestablishment. Moreover, somatic embryogenesis resulted in a higher level of genomic DNA demethylation in regenerants than androgenesis and the process of methylation seems to be affected by donor plant. It is being speculated that long term changes in genomic DNA methylation may be a source of off-type individuals that may spontaneously arise during plant breeding.

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“…6 × 10 6 of haploid cells capable of synchronous development can be cultured in a small volume (1 ml) of medium (Jähne & Lörz 1995;Oleszczuk et al 2006). The relevant literature lacks a definite answer which of these two methods yields better results not only in barley (Li & Devaux 2003;Lantos et al 2014). In some reports, the number of green plants (GP) obtained via IMC was up to nine times higher than in AC (Li & Devaux 2005).…”

mentioning

confidence: 99%

The effect of copper on plant regeneration in barley microspore culture

Makowska¹,

Oleszczuk²,

Zimny³

2017

CZECH J GENET PLANT

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Makowska K., Oleszczuk S., Zimny J. (2017): The effect of copper on plant regeneration in barley microspore culture. Czech J. Genet. Plant Breed., 53: 17-22.Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.

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Comparative analysis of in vitro anther- and isolated microspore culture in hexaploid Triticale (X Triticosecale Wittmack) for androgenic parameters

Cited by 39 publications

References 29 publications

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

The effect of copper on plant regeneration in barley microspore culture

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