Comparative analysis of honey and citrate stabilized gold nanoparticles: In vitro interaction with proteins and toxicity studies

Brăgaru, Adina; Simion, Monica; Mihalache, Iuliana; Radoi, Antonio; Banu, Melania; Varasteanu, Pericle; Nadejde, Paul; Vasile, Eugeniu; Acasandrei, A.; Popescu, Roxana Cristina; Savu, Diana; Kusko, Mihaela

doi:10.1016/j.jphotobiol.2019.111519

Cited by 38 publications

(29 citation statements)

References 67 publications

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“…GNPs have been used to treat a number of diseases by virtue of their biocompatibility and unique properties, such as conductivity, optical catalytic activity, and better structural characteristics than their bulk counterparts. GNP-based marine algae have been investigated thoroughly, and it has been experimentally documented that they possess medicinal properties, as well as various biological effects, such as antioxidative, antibacterial, anti-inflammatory, and antitumor activities [15][16][17][18]. Ecklonia stolonifera is a brown alga of the Laminariaceae family that is mainly distributed in the sea forests off the coasts of Far Eastern countries, such as Korea, China, and Japan [19].…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3

Jun

Kim

et al. 2020

Marine Drugs

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The effect of gold nanoparticles (GNPs) synthesized in marine algae has been described in the context of skin, where they have shown potential benefit. Ecklonia stolonifera (ES) is a brown algae that belongs to the Laminariaceae family, and is widely used as a component of food and medicine due to its biological activities. However, the role of GNPs underlying cellular senescence in the protection of Ecklonia stolonifera gold nanoparticles (ES-GNPs) against UVA irradiation is less well known. Here, we investigate the antisenescence effect of ES-GNPs and the underlying mechanism in UVA-irradiated human dermal fibroblasts (HDFs). The DPPH and ABTS radical scavenging activity of ES extracts was analyzed. These analyses showed that ES extract has potent antioxidant properties. The facile and optimum synthesis of ES-GNPs was established using UV-vis spectra. The surface morphology and crystallinity of ES-GNPs were demonstrated using high resolution transmission electron microscopy (HR-TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). ES-GNPs presented excellent photocatalytic activity, as shown by the photo-degradation of methylene blue and rhodamine B. A cellular senescence model was established by irradiating HDFs with UVA. UVA-irradiated HDFs exhibited increased expression of senescence-associated β-galactosidase (SA-β-galactosidase). However, pretreatment with ES-GNPs resulted in reduced SA-β-galactosidase activity in UVA-irradiated HDFs. Intracellular ROS levels and G1 arrest in UVA-irradiated HDFs were checked against the background of ES-GNP treatment to investigate the antisenescence effects of ES-GNPs. The results showed that ES-GNPs significantly inhibit UVA-induced ROS levels and G1 arrest. Importantly, ES-GNPs significantly downregulated the transcription and translation of MMP (matrix metalloproteinases)-1/-3, which regulate cellular senescence in UVA-irradiated HDFs. These findings indicate that our optimal ES-GNPs exerted an antisenescence effect on UVA-irradiated HDFs by inhibiting MMP-1/-3 expression. Collectively, we posit that ES-GNPs may potentially be used to treat photoaging of the skin.

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Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3

Jun

Kim

et al. 2020

Marine Drugs

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“…This spectroscopic technique allows detecting aggregation processes of nanomaterials or absorption of molecules onto nanoparticle surfaces that give rise to the modification of their absorption spectra. 66,67 Normalized UV−vis spectra of AuVmNPs and CAuNPs in PBS and water are shown in Figure S7A for comparison. For AuVmNPs, there are no relevant changes in the shape of the spectrum or the position of LSPR (537 nm).…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…For this, the behavior of localized surface plasmon resonance (LSPR) of the gold nanoparticles in PBS and DMEM was evaluated by UV–vis spectroscopy when dispersing them at a concentration of 200 μg/mL. This spectroscopic technique allows detecting aggregation processes of nanomaterials or absorption of molecules onto nanoparticle surfaces that give rise to the modification of their absorption spectra. , …”

Section: Resultsmentioning

confidence: 99%

Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized from Vitex mollis

et al. 2021

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A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract of Vitex mollis fruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet–visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.

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“…Similarly to the increase in the nanoparticle size and surface charge reversal observed in our study, Li et al (2021) reported that humic acid adsorption on AuNPs increases their effective size and reverses nanoparticle surface charge. Further, Boldeiu et al (2019) reported that citrate- and honey-capped AuNPs suspended in culture media supplemented with 10% FBS resulted in the formation of PC around AuNPs, which promoted agglomeration and clustering of the AuNPs. Moreover, it has been observed that the kinetics of PC formation and the characteristics of PC are based on the particle size as well as their shape ( Piella et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Reduced Genotoxicity of Gold Nanoparticles With Protein Corona in Allium cepa

Arya

Rookes

Cahill

et al. 2022

Front. Bioeng. Biotechnol.

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Increased usage of gold nanoparticles (AuNPs) in biomedicine, biosensing, diagnostics and cosmetics has undoubtedly facilitated accidental and unintentional release of AuNPs into specific microenvironments. This is raising serious questions concerning adverse effects of AuNPs on off-target cells, tissues and/or organisms. Applications utilizing AuNPs will typically expose the nanoparticles to biological fluids such as cell serum and/or culture media, resulting in the formation of protein corona (PC) on the AuNPs. Evidence for PC altering the toxicological signatures of AuNPs is well studied in animal systems. In this report, we observed significant genotoxicity in Allium cepa root meristematic cells (an off-target bioindicator) treated with high concentrations (≥100 µg/ml) of green-synthesized vanillin capped gold nanoparticles (VAuNPs). In contrast, protein-coated VAuNPs (PC-VAuNPs) of similar concentrations had negligible genotoxic effects. This could be attributed to the change in physicochemical characteristics due to surface functionalization of proteins on VAuNPs and/or differential bioaccumulation of gold ions in root cells. High elemental gold accumulation was evident from µ-XRF mapping in VAuNPs-treated roots compared to treatment with PC-VAuNPs. These data infer that the toxicological signatures of AuNPs are influenced by the biological route that they follow to reach off-target organisms such as plants. Hence, the current findings highlight the genotoxic risk associated with AuNPs, which, due to the enhanced utility, are emerging as new pollutants. As conflicting observations on the toxicity of green-synthesized AuNPs are increasingly reported, we recommend that detailed studies are required to investigate the changes in the toxicological signatures of AuNPs, particularly before and after their interaction with biological media and systems.

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Comparative analysis of honey and citrate stabilized gold nanoparticles: In vitro interaction with proteins and toxicity studies

Cited by 38 publications

References 67 publications

Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3

Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3

Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized from Vitex mollis

Reduced Genotoxicity of Gold Nanoparticles With Protein Corona in Allium cepa

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