Comparative analysis of gut microbiota of mosquito communities in central Illinois

Muturi, Ephantus J.; Ramirez, José L.; Rooney, Alejandro P.; Kim, Chang‐Hyun

doi:10.1371/journal.pntd.0005377

Cited by 109 publications

(130 citation statements)

References 73 publications

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“…Recent similar methods using 16S rRNA amplicon sequencing to determine the overall microbiota in wild mosquito populations has provided evidence for Wolbachia infections in An. gambiae in additional villages in Burkina Faso [54] and Anopheles species collected in Illinois, USA [55]. Our study describing resident Wolbachia strains in numerous species of Anopheles malaria vectors also highlights the potential for Wolbachia to be influencing malaria transmission, as postulated by previous studies [39,40,56].…”

Section: The Mosquito Microbiota Can Modulate the Mosquito Immune Ressupporting

confidence: 75%

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Jeffries

Golovko

Kristan

et al. 2018

Preprint

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Anopheles (An.) mosquitoes contain bacteria that can influence Plasmodium parasites. Wolbachia, a common insect endosymbiont, has historically been considered absent from Anopheles but has recently been found in An. gambiae populations. Here, we assessed a range of Anopheles species from five malaria-endemic countries for Wolbachia and Plasmodium infection. Strikingly, we found Wolbachia infections in An. coluzzii, An. gambiae s.s, An. arabiensis, An. moucheti and An. species ‘A’ increasing the number of Anopheles species known to be naturally infected by this endosymbiont. Molecular analysis suggests the presence of phylogenetically diverse novel strains, while qPCR and 16S rRNA sequencing indicates that Wolbachia is the dominant member of the microbiota in An. moucheti and An. species ‘A’. We found no evidence of Wolbachia/Asaia co-infections, and presence of these endosymbionts did not have significant effects on malaria prevalence. We discuss the importance of novel Wolbachia strains in Anopheles and potential implications for disease control.

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Section: The Mosquito Microbiota Can Modulate the Mosquito Immune Ressupporting

confidence: 75%

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Jeffries

Golovko

Kristan

et al. 2018

Preprint

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“…The results suggested that Burkholderia and Ralstonia might be the vital components of the gut bacteria in the three spider species, and the relative abundance of these two bacteria in the spider guts may affect the distribution and abundance of the other bacteria. In regard to other bacteria identified in this study (more than 0.1% of total reads), Propionibacterium, Morganella, Providencia, Sphingomonas, Chryseobacterium, and Corynebacterium were also found in the guts of mosquitoes (Charan et al, 2016;Manguin, 2013;Muturi et al, 2017). Lactococcus, Lactobacillus, Pseudomonas, Paracoccus, Microbacterium, Serratia, Achromobacter, Bacillus, and Agrobacterium were also found in the guts of Lepidoptera (Chen et al, 2016;Snyman et al, 2016), and Serratia are known to contain hemolytic enzymes and could play a potential role in blood digestion (Gaio et al, 2011) Moran, & Hunter, 2003).…”

Section: Discussionmentioning

confidence: 67%

“…In recent years, researchers have become increasingly interested in the gut bacteria of arthropods with the advent of next‐generation sequencing (NGS) technology. The diversity of gut bacteria has been studied in a range of insects and ticks (Anjum et al, ; Muturi, Ramirez, Rooney, & Kim, ; Snyman, Gupta, Bezuidenhout, Claassens, & Van dBJ, ; Waite et al, ). Gut bacteria have a great impact on their insect hosts, such as resistance against parasites and pathogens (Dillon, Vennard, & Charnley, ; Koch & Schmid‐Hempel, ), nutrient supplementation (Eichler & Schaub, ; Hongoh et al, ), intestinal cell renewal and developmental rate (Shin et al, ), and digestion of dietary compounds (Gaio et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, researchers have become increasingly interested in the gut bacteria of arthropods with the advent of next-generation sequencing (NGS) technology. The diversity of gut bacteria has been studied in a range of insects and ticks (Anjum et al, 2018;Muturi, Ramirez, Rooney, & Kim, 2017;Snyman, Gupta, Bezuidenhout, Claassens, & Van dBJ, 2016;Waite et al, 2015). Gut bacteria have a great impact on their insect throughout the world.…”

Section: Introductionmentioning

confidence: 99%

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Taking insight into the gut microbiota of three spider species: No characteristic symbiont was found corresponding to the special feeding style of spiders

Zhang

Yao

et al. 2019

Ecology and Evolution

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Microorganisms in insect guts have been recognized as having a great impact on their hosts' nutrition, health, and behavior. Spiders are important natural enemies of pests, and the composition of the gut microbiota of spiders remains unclear. Will the bacterial taxa in spiders be same as the bacterial taxa in insects, and what are the potential functions of the gut bacteria in spiders? To gain insight into the composition of the gut bacteria in spiders and their potential function, we collected three spider species, Pardosa laura , Pardosa astrigera, and Nurscia albofasciata , in the field, and high‐throughput sequencing of the 16S rRNA V3 and V4 regions was used to investigate the diversity of gut microbiota across the three spider species. A total of 23 phyla and 150 families were identified in these three spider species. The dominant bacterial phylum across all samples was Proteobacteria. Burkholderia , Ralstonia , Ochrobactrum , Providencia , Acinetobacter , Proteus, and Rhodoplanes were the dominant genera in the guts of the three spider species. The relative abundances of Wolbachia and Rickettsiella detected in N . albofasciata were significantly higher than those in the other two spider species. The relative abundance of Thermus , Amycolatopsis , Lactococcus , Acinetobacter Microbacterium, and Koribacter detected in spider gut was different among the three spider species. Biomolecular interaction networks indicated that the microbiota in the guts had complex interactions. The results of this study also suggested that at the genus level, some of the gut bacteria taxa in the three spider species were the same as the bacteria in insect guts.

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“…Recent similar methods using 16S rRNA amplicon sequencing to determine the overall microbiota in wild mosquito populations has provided evidence for Wolbachia infections in An. gambiae in additional villages in Burkina Faso 71 and Anopheles species collected in Illinois, USA 72 . Our study describing resident Wolbachia strains in numerous species of Anopheles malaria vectors also highlights the potential for Wolbachia to be influencing malaria transmission, as postulated by previous studies 39, 40, 73 .…”

Section: Discussionmentioning

confidence: 99%

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Jeffries¹,

Lawrence²,

Golovko³

et al. 2018

Wellcome Open Res

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Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing endosymbiotic bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis of samples was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species ‘A’, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species ‘A’. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species ‘A’, but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were also shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.

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Comparative analysis of gut microbiota of mosquito communities in central Illinois

Cited by 109 publications

References 73 publications

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

Taking insight into the gut microbiota of three spider species: No characteristic symbiont was found corresponding to the special feeding style of spiders

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa

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