Comparative Analysis of GS2 and Fd-GOGAT Genes in Cultivated Wheat and Their Progenitors Under N Stress

Gayatri,; Jayaraman, Karikalan; Sinha, S. K.; Roy, Priyanka; Mandal, Pranab Kumar

doi:10.1007/s11105-020-01267-2

Cited by 8 publications

(5 citation statements)

References 54 publications

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“…N stress decelerated all the morphological parameters and photosynthetic pigment/chlorophyll and carotenoid content except RL and RFW. While on the one hand, we found the effect of N stress on enzymatic activity, we also observed wide genotypic variation, both under ON and SN, implying that they had diverse adaptive mechanisms to regulate N and C assimilation (Chandna et al., 2010; Gayatri et al., 2021; Sinha et al., 2015). PCA analysis clearly depicted chlorophyll contents and morphological parameters as essential factors contributing to the variations that occurred under both ON and SN conditions.…”

Section: Discussionmentioning

confidence: 65%

“…Thereby GDH had to increase its activity to produce more 2‐oxoglutarate (for TCA cycle) by catabolizing glutamate. However, the lack of correlation between the enzymatic activity and their gene expression pattern in the case of NiR, CS and ICDH is possibly due to post‐transcriptional and post‐translational modifications (Gayatri et al., 2021; Kant et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…RT‐qPCR primers for all the genes were designed from the conserved region of the coding sequences of a gene (Table S3). The expression of each gene was calculated as relative fold change using the relative 2 −ΔΔCt method (Gayatri et al., 2021; Schmittgen & Livak, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative real-time PCR (qRT-PCR). The expression pattern for all genes was determined using qRT-PCR (Gayatri et al, 2021). RT-qPCR primers for all the genes were designed from the conserved region of the coding sequences of a gene (Table S3).…”

Section: Gene Expression Study Of the Enzymesmentioning

confidence: 99%

“…Seventeen representative genotypes were grown hydroponically for 15 days under optimum N (ON: 8 mmol N/L) and stressed N (SN: 0.08 mmol N/L) conditions in the culture room (Gayatri et al, 2021(Gayatri et al, , 2019. Seedlings were harvested after 15 days of growth, and shoots and roots were separated and stored at −80°C after snap freezing in liquid nitrogen.…”

Section: Hydroponics Experiments and Measured Parametersmentioning

confidence: 99%

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Unravelling the interplay of different traits and parameters related to nitrogen use efficiency in wheat for climate‐resilient agriculture

Gayatri,

Mandal,

Venkatesh

et al. 2024

J Agronomy Crop Science

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Enhancing Nitrogen Use Efficiency (NUE) is extremely important towards mitigating climate change, especially in wheat where the NUE is less than 50%. Hence, optimizing grain yield under reduced application of nitrogenous fertilizer is a significant challenge. To address this challenge, a comprehensive study was conducted to investigate various agronomic traits and morphological, biochemical and molecular parameters related to NUE. This study explored their interrelationships and effects on grain yield, providing novel insights that were not previously reported. A set of 278 diverse wheat genotypes were assessed, encompassing eight NUE‐related field traits. All traits' values were reduced under stressed N (ranging from 7.5% to 77.5%) except Nitrogen Utilization Efficiency (NUtE) and NUE. Data analysis showed a significant positive correlation between grain yield and all other NUE‐related traits (r2 value ranged from .23 to 1.00), highlighting their relevance in comprehending the biological NUE of wheat plants. Principal component analysis (PCA) also revealed that N at head and N at harvest were more connected with gain yield, NUE and biomass under the optimum N condition, but less connected with gain yield and NUE under the stressed N condition. To complement the field data, representative genotypes were further subjected to a hydroponics experiment under absolute N control to study the different morphological parameters, photosynthetic pigments and the performance of essential N‐ and C‐metabolizing enzymes at the seedling stage. N stress had a detrimental impact on the majority of the parameters (−0.84% to −79.8%). Nitrite reductase (NiR), glutamate dehydrogenase (GDH) and isocitrate dehydrogenase (ICDH) enzymes as well as root length (RL), root fresh weight (RFW) and CS transcript, were positively affected by 5.9%–35.6%. The correlation analysis highlighted the substantial influence of four key N‐metabolizing enzymes, namely nitrate reductase (NR), glutamine synthetase (GS), glutamate oxo‐glutarate aminotransferase (GOGAT), and GDH on grain yield. Additionally, this study highlighted the direct and indirect associations between seedling parameters and field traits, where shoot and root length were found to be most significant for N acquisition, especially under N stress. In conclusion, these findings offer valuable insights into the intricate network of traits and parameters influencing wheat grain yield under varying N regimes.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%