Comparative analysis of Gram's stain, <scp>PNA</scp>‐<scp>FISH</scp> and Sepsityper with <scp>MALDI</scp>‐<scp>TOF MS</scp> for the identification of yeast direct from positive blood cultures

Gorton, Rebecca; Ramnarain, P.; Barker, Kevin R.; Stone, Neil; Rattenbury, S; McHugh, Timothy D.; Kibbler, C

doi:10.1111/myc.12205

Cited by 35 publications

(28 citation statements)

References 42 publications

(108 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Triple probes detecting T. vaginalis, G. vaginalis, and Candida (BD Affirm VPIII) have grown massively in popularity but are not inexpensive. PNA FISH probes exist for Candida species, but so far have been applied to their detection after they grow in blood cultures [14][15][16].…”

Section: Diagnostics For Vulvovaginal Candidiasismentioning

confidence: 99%

The Role of PCR in the Diagnosis of Candida Vulvovaginitis—a New Gold Standard?

Sobel

Akins

2015

Curr Infect Dis Rep

View full text Add to dashboard Cite

PCR is recognized as a reliable technique for detection of all types of microorganisms. Being highly objective and reproducible also sensitive and specific, PCR is now widely used for sexually transmitted infection (STI) diagnosis. Potential, however, exists for detecting non-pathogens, and not identifying a pathogenic state decreases specificity or clinical significance. PCR Candida tests of vaginal specimens are now widely available and frequently used offering a modest to moderate increase in sensitivity and are likely to replace traditional culture and DNA homology testing. Nevertheless, there remain considerable gaps in our knowledge regarding the usefulness and applications of these expensive tests.

show abstract

Section: Diagnostics For Vulvovaginal Candidiasismentioning

confidence: 99%

The Role of PCR in the Diagnosis of Candida Vulvovaginitis—a New Gold Standard?

Sobel

Akins

2015

Curr Infect Dis Rep

View full text Add to dashboard Cite

show abstract

“…Etken Candida'nın tür düzeyinde tanımlan-ması, bazı antifungal ilaçlar ve türler için gözle-nen türe özgü primer direnç varlığı nedeniyle tedavi kararını etkilemektedir. Konvansiyonel tür tanımlama yöntemlerinin yanı sıra, literatür-de kan dolaşımı infeksiyonlarında fungal etkenin erken tanısı ve tanımlanması için serolojik belirteçler, polimeraz zincir reaksiyonu veya floresan hibridizasyon probları gibi farklı yön-temleri kullanan ve etkinliğini araştıran çalış-malar bulunmaktadır (5,8,11,19) . Bazı türler için geçerli olmak üzere, tür tanımlama süresinin kısaltılması amacıyla hasta örnekleri kromojenik besiyerlerine ekilebilmekte veya maya kolonilerine enzimatik testler uygulanabilmektedir (13,18) .…”

Section: Introductionunclassified

A Fast Preliminary Identification Test in Fungemia: Evaluation of Germ Tube Test Directly from Positive Blood Culture Bottles

Doğan¹,

Gülmez²,

Akdağlı³

2016

Ankem Derg

View full text Add to dashboard Cite

ÖZET SUMMARY A Fast Preliminary Identification Test in Fungemia: Evaluation of Germ Tube Test Directly from Positive Blood Culture Bottles The incidence of candidemia tends to increase in high risk patient populations in most centers and it presents with high mortality. The causative agent is generally Candida albicans; however, frequency of other Candida species is rising with variations among centers. Blood culture is the gold standard to diagnose candidemia and definitive identification of the causative agent requires positive signal in automated blood culture system, subculture on solid media and examination of growth via conventional methods. When germ tube test (GTT) is performed directly from yeast positive blood culture bottles ("direct GTT"), on the other hand, C.albicans and Candida dubliniensis may be identified one day earlier as compared to the conventional methods, providing an opportunity for an earlier appropriate antifungal therapy and decrease in mortality. The aim of this study was to investigate the correlation of the results obtained by direct GTT with those by conventional identification methods. Yeast positive blood culture bottles that were referred to the Mycology Laboratory within the time period of October 2014 and July 2016 were included the study. GTT was performed directly from the bottles and colonies obtained from subcultures onSabouraud dextrose agar and chromogenic agar were identified by conventional methods. A total of 172 blood culture specimens from 169 patients were evaluated during the study period. Of these, 151 (87.8 %)

show abstract

“…This adaptation of MALDI-TOF MS can be performed in-house or in conjunction with a commercially available Sepsityper kit (Bruker Daltonics, Billerica, MA). Using Sepsityper, red blood cells are lysed, centrifuged, and washed to create a concentrated yeast pellet which is then analyzed using MALDI-TOF MS. An increasing number of reports on this topic have demonstrated a wide range of sensitivity (0-100 %) [41][42][43][44][45][46] to identify Candida to the species level. The authors of one of the largest such reports, using an in-house method to prepare positive blood culture broth for subsequent MALDI-TOF MS analyzed by the Bruker Biotyper software (version 2.0), found a sensitivity of 91 % (316/376) and attributed much of the variability reported to significant methodologic differences across studies [41].…”

Section: Maldi-tof Msmentioning

confidence: 98%