Comparative analysis of gene sequence of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland

Ramunno, L.; Cosenza, G.; Rando, A.; Pauciullo, Alfredo; Illario, Rosa; Gallo, Daniela; Berardino, D. Di; Masina, P.

doi:10.1016/j.gene.2004.12.003

Cited by 83 publications

(92 citation statements)

References 26 publications

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“…In Figure 2 can be observed genotypes after XmnI digestion of fragments obtained by the XmnI PCR-RFLP method according to Ramunno et al (2000). This technique allowed us to identify F and N alleles, but not distinguish alleles A from G, H, I, O 1 , O 2 on the haplotype associated to 63 and 150 bp fragments, neither alleles B from B 1 , B 2 , B 3 , B 4 , C, E, L on the haplotype associated to 63 and 161 bp fragments (Ramunno et al 2005;Cosenza et al 2008). …”

Section: E F and N Allele Genotypingmentioning

confidence: 99%

“…The alleles of the CSN1S1 locus in goats have been grouped in 4 levels according to their individual approximate contribution to quantity of protein in milk; strong alleles (A, B 1 , B 2 , B 3 , B 4 , C, H, L and M), intermediate alleles (E and I), weak alleles (D, F and G), and null alleles (O 1 , O 2 and N) (Grosclaude et al 1987;Moioli et al 1998;Rando et al 2000;Bevilacqua et al 2002;Ramunno et al 2005;Sacchi et al 2005;Sztankóová et al 2007). In dairy goat populations, it is often considered convenient to identify the CSN1S1 genotypes of individual animals, with the idea of incorporating this information to the selection processes, and therefore propitiate an increase on the rate of genetic gain for casein contents and yield through gene assisted selection (GAS), compared to selection programs based only on phenotypic and pedigree information (BLUP), (Grosclaude et al 1987;Moioli et al 1998;Dekkers, 2004;Sánchez et al 2005).…”

mentioning

confidence: 99%

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Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Torres‐Vázquez¹,

Flores²,

Montaldo³

et al. 2008

Electron. J. Biotechnol.

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Section: E F and N Allele Genotypingmentioning

confidence: 99%

mentioning

confidence: 99%

Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Torres‐Vázquez¹,

Flores²,

Montaldo³

et al. 2008

Electron. J. Biotechnol.

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“…The caprine LALBA gene has been less frequently studied than the goat casein genes and their polymorphisms due to limited economic interests [8][9][10][11]. Initial reports regarding various LALBA forms emerged in the Italian population [11] and later, in Chinese breeds, different variants were analysed for influence on milk and body size traits [4].…”

Section: Introductionmentioning

confidence: 99%

Allele mining in the caprine alpha-lactalbumin (LALBA) gene of native Saudi origin

El-Hanafy

Qureshi

Sabir

et al. 2016

Biotechnology & Biotechnological Equipment

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Alpha-lactalbumin (a-LA) is a distinctive whey protein found in bovine milk and the milk of other mammalian species. Its main function in the lactating mammary glands is involved in lactose biosynthesis, which makes it a potential quantitative trait locus. The aim of this study is to determine the incidence of single nucleotide polymorphisms (SNPs) within the exonic and intronic portions of the caprine a-LA gene (LALBA) in native Saudi breeds (Ardi, Habsi and Harri) in relation to utility traits. Blood samples were collected from 300 animals (100 for each breed). Genomic DNA was extracted and a 268 bp fragment of the LALBA gene comprising exon 3 and its flanking area was amplified. Subsequent digestion with MvaI restriction endonuclease resulted in two different banding patterns: aLA A1 /aLA A1 and aLA A1/aLA A2 , and two allelic forms, i.e. aLA A1 and aLA A2 . Nucleotide sequencing of the designated LALBA amplicons was done and, following successful BLAST analysis, the sequences were submitted to GenBank (NCBI Accession No. KM267632, KM267633, KM267634 and KP940442). SNPs were searched within breeds of Saudi origin and homology was sought among caprine, ovine and bovine species. A C > T transition was identified within the fifth nucleotide of the silent a-LA A2 allele. Phylogenetic analysis on the basis of LALBA nucleotide sequence of Saudi goats indicated similarity with evolutionarily related sheep, while the origin of bovine species and deer was located some distance away.

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“…Seventeen α s1 -casein variants associated with high α s1 -casein content (3.6 g·L -1 per allele; A, B1, B2, B3, B4, C, L and M or 4.2 g·L -1 per allele, H) or medium (1.6 g·L -1 per allele; E and I) or low (0.6 g·L -1 per allele; D, F and G) or no α s1 -casein (O 1 , O 2 and N) have been so far reported [4,28,42]. An additional source of α s1 -casein polymorphism is the simultaneous existence of deleted forms due to alternative splicing [16].…”

Section: Introductionmentioning

confidence: 99%

Protein composition and polymorphism in the milk of Skopelos goats

Moatsou

Vamvakaki

Mollé

et al. 2006

Lait

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-Individual milk samples taken from goats of the Skopelos breed of Greece were analyzed by RP-HPLC using as standards the casein fraction of milks of known genotypes. Milk samples with particular characteristics with respect to the casein fraction were further analyzed by IEF and RP-HPLC/ESI-MS. The mean total protein content (36.7 ± 2.6 g·L -1 ) and the mean casein content (29.7 ± 2.3 g·L -1 ) were high. The same was true for the α s1 -cn fraction, which was 21.8% of the total cn fraction, whereas the β-cn percentage of total cn was 43.8%. The respective values for α s2 -cn and κ-cn were 13.7% and 13.8%. These quantitative characteristics were consistent with the predominance of the strong α s1 -cn variants B3, B4 and As/B1. In all samples analyzed by RP-HPLC/ ESI-MS the deleted form differing from the respective complete form by Gln78 was found at different levels of phosphorylation, with the exception of variants F and D/G. The most frequent α s2 -cn variant was by far variant A followed by variants C and F, whereas compositional data implied the existence of a null allele. The detected β-cn forms were variants A and C in similar frequencies. Variant D (formerly B) of κ-cn predominated and the rare variant G was found. Different phosphorylation types of each casein variant and the characteristics of whey proteins were also determined.goat milk protein / casein genotype / phosphorylation / protein quantification / polymorphism Résumé -Polymorphisme des protéines du lait de chèvre de la race Skopelos. Des laits individuels de chèvres de Skopelos (Grèce) ont été analysés par RP-HPLC et les profils protéiques ont été comparés avec ceux issus de lait de chèvres de génotypes connus au locus α s1 . Les échantillons qui présentaient des caractéristiques particulières de leur fraction caséique ont été analysés par IEF et RP-HPLC/ESI-MS. Les teneurs moyennes en protéines totales (36,7 ± 2,6 g·L -1 ), et en caséines (29,7 ± 2,3 g·L -1 ), étaient plus élevées que dans les laits des races hautement sélectionnées. Le pourcentage en α s1 -cn représentait 21,8 % des caséines totales, tandis que celui de la β-cn atteignait 43,8 %. Les pourcentages en α s2 -cn et κ-cn étaient respectivement 13,7 % et 13,8 %. Ces caracté-ristiques quantitatives étaient corrélées avec la prédominance des variants forts B3, B4 et As/B1 de la α s1 -cn. Dans tous les échantillons analysés par RP-HPLC/ESI-MS, la forme déplétée en Gln78 de la α s1 -cn présentait plusieurs niveaux de phosphorylation, à l'exception des variants F et D/G. Le variant le plus abondant de la α s2 -cn était le variant A suivi par les variants C et F. Les variants A et C de la β-cn ont été trouvés en fréquences similaires. Le variant D (antérieurement dénommé B) de la κ-cn était prédominant bien que le variant rare G était aussi trouvé. Plusieurs niveaux de phosphorylation de chacun de ces variants ont été aussi mis en évidence, ainsi que les principales caractéristiques des protéines du sérum.polymorphisme / variant proteique / lait de chèvre / caséine / génotype /...

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Comparative analysis of gene sequence of goat CSN1S1 F and N alleles and characterization of CSN1S1 transcript variants in mammary gland

Cited by 83 publications

References 26 publications

Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Genetic polymorphism of the as1-casein locus in five populations of goats from Mexico

Allele mining in the caprine alpha-lactalbumin (LALBA) gene of native Saudi origin

Protein composition and polymorphism in the milk of Skopelos goats

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