Comparative Analysis of Gene Expression Analysis Methods for RNA In Situ Hybridization Images
Valeria Ariotta,
Eros Azzalini,
Vincenzo Canzonieri
et al.
Abstract:Gene expression analysis is pivotal in cancer research and clinical practice. While traditional methods lack spatial context, RNA in situ hybridization (RNA-ISH) is a powerful technique that retains spatial tissue information. Here, we investigated RNAscope score, RT-droplet digital PCR (RT-ddPCR), and automated QuantISH and QuPath in quantifying RNA-ISH expression values from formalin-fixed paraffin-embedded samples. We compared the methods using high-grade serous ovarian carcinoma samples, focusing on CCNE1,… Show more
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