Comparative analysis of gene expression in brain, liver, skeletal muscles, and gills of zebrafish (<i>Danio rerio</i>) exposed to environmentally relevant waterborne uranium concentrations

Lerebours, Adélaïde; Gonzalez, Patrice; Adam, Christelle; Camilleri, Virginie; Bourdineaud, Jean‐Paul; Garnier‐Laplace, Jacqueline

doi:10.1897/08-357.1

Cited by 57 publications

(43 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonetheless, the overall capacity of zebrafish to (totally) eliminate uranium still remains an open question. Uranium was not entirely eliminated in muscle, liver, gills or brain after a week (Lerebours et al, 2009) nor in whole body residues after 30 days (Labrot et al, 1999). However, uranium was eliminated from muscle tissue of goldfish Carassius auratus previously exposed for 4 days (Lourenço et al, 2010).…”

Section: Elimination and Uptakementioning

confidence: 91%

Effects of uranium on the metabolism of zebrafish, Danio rerio

et al. 2012

View full text Add to dashboard Cite

The increasing demand for nuclear energy results in heightened levels of uranium (U) in aquatic systems which present a potential health hazard to resident organisms. The aim of this study was to mechanistically assess how chronic exposure to environmentally relevant concentrations of U perturbs the complex interplay between feeding, growth, maintenance, maturation and reproduction throughout the life-cycle of an individual. To this end we analysed literature-based and original zebrafish toxicity data within a same mass and energy balancing conceptual framework. U was found to increase somatic maintenance leading to inhibition of spawning as well as increase hazard rate and costs for growth during the early life stages. The fish's initial conditions and elimination through reproduction greatly affected toxico-kinetics and effects. We demonstrate that growth and reproduction should be measured on specific individuals since mean values were hardly interpretable. The mean food level differed between experiments, conditions and individuals. This last 'detail' contributed substantially to the observed variability by its combined effect on metabolism, toxic effects and toxico-kinetics. The significance of this work is that we address exactly how these issues are related and derive conclusions which are independent of experimental protocol and coherent with a very large body of literature on zebrafish eco-physiology.

show abstract

Section: Elimination and Uptakementioning

confidence: 91%

Effects of uranium on the metabolism of zebrafish, Danio rerio

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Although uranium is a radionuclide, its toxic effects and uptake are controlled by its chemical properties. The accumulation levels and toxic effects of this element have been evaluated in several biological models [21][22][23][24]. In crayfish, uranium can impair mitochondrial function and induce oxidative stress [23].…”

Section: Introductionmentioning

confidence: 99%

Relative importance of direct and trophic uranium exposures in the crayfish Orconectes limosus: Implication for predicting uranium bioaccumulation and its associated toxicity

Simon

Floriani

Camilleri

et al. 2012

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Abstract-Pollutants that occur at sublethal concentrations in the environment may lead to chronic exposure in aquatic organisms. If these pollutants bioaccumulate, then organisms higher in the food chain may also be at risk. Increased attention has thus been focused on the relative importance of dietary uptake, but additional knowledge of the cellular distribution of metals after dietary exposure is required to assess the potential toxicity. The authors address concerns relating to increasing uranium (U) concentrations (from 12 mg/L to 2 mg/L) in the freshwater ecosystem caused by anthropogenic activities. The objective of the present study is to compare uranium bioaccumulation levels in tissues and in the subcellular environment. The authors focused on the cytosol fraction and its microlocalization (TEM-EDX) in the gills and the hepatopancreas (HP) of the crayfish Orconectes limosus after 10 d of direct exposure (at concentrations of 20, 100, and 500 mg/L) and five trophic exposure treatments (at concentrations from 1 to 20 mg/g). Results indicated that adsorption of uranium on the cuticle represents the main contribution of total uranium accumulation to the animal. Accumulation in the gills should be considered only as a marker of waterborne uranium exposure. Accumulation in the HP after trophic environmental exposure conditions was higher (18.9 AE 3.8 mg/g) than after direct exposure. Moreover, no significant difference in the subcellular distribution of uranium (50%) in HP was observed between animals that had been exposed to both types of treatment. A potential toxic effect after uranium accumulation could therefore exist after trophic exposure. This confirms the need to focus further studies on the metal (uranium) risk assessment. Environ. Toxicol. Chem. 2013;32:410-416. # 2012 SETAC

show abstract

“…The rad51 and p53 genes investigated were induced up to about 1.5-fold, and this induction was consistent with the relatively low levels of induction observed in other studies (Reinardy et al 2013). However, rad51 can be highly induced in adult zebrafish, and a 32-fold change in expression has been noted in liver after a 7 d exposure to cadmium (Gonzalez et al 2006) and 5-fold change in gills after an 8 d exposure to uranium (Lerebours et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles

Boran

Ulutas²

2016

Dis. Aquat. Org.

View full text Add to dashboard Cite

Engineered nanoparticles (NPs) can potentially generate adverse effects at the tissue, organ, cellular, subcellular, DNA, and protein levels due to their unique physico-chemical properties. Dissoluble NPs (e.g. nZnO) can be toxic in aquatic organisms. We compared effects of nZnO and corresponding concentrations of released Zn(II) by water-soluble ZnCl 2 on larval zebrafish Danio rerio (72 h post fertilization) by analyzing changes in expression levels of stressrelated genes ( p53, rad51, mt2) by qRT-PCR. Additionally, genotoxicity of nZnO and Zn(II) was assessed. The lethal concentrations for 50% mortality (LC 50 ) in larval zebrafish exposed for 96 h to 0 to 70 mg l −1 nZnO and Zn(II) were 21.37 ± 1.81 mg l −1 (95% CI) and 4.66 ± 0.11 mg l −1 , respectively. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed (96 h) to nZnO and Zn(II). DNA damage was higher in Zn(II)-than nZnO-exposed larvae. Induction of stress-related genes in larvae was complex and was not directly related to nZnO and Zn(II) concentrations, although there was significant induction in the mt2 gene of larvae exposed to Zn(II) and nZnO relative to controls. mt2 induction of 20.5 ± 1.9-fold and 2.5 ± 0.8-fold change (mean ± SEM) was observed in larvae at the highest Zn(II) and nZnO concentrations (3 and 6 mg l −1 ), respectively. The results suggest that toxicity associated with nZnO is primarily due to the release of Zn(II).KEY WORDS: Nanoscale ZnO · Zinc chloride · Genotoxicity · Stress-related genes · Danio rerio Resale or republication not permitted without written consent of the publisherDis Aquat Org 117: [205][206][207][208][209][210][211][212][213][214] 2016 for a number of enzymes. However, when present in excessive concentrations, this metal can cause adverse effects (Regoli & Principato 1995, Wilson et al. 2012. nZnO can partially but relatively rapidly dissolve in water, and the release of free Zn ions is the primary source of toxicity (Franklin et al. 2007, Blinova et al. 2010, Buerki-Thurnherr et al. 2013. Several studies have indicated that nZnO are toxic to fish: mortality, hatching inhibition, retarded growth, tissue injuries, and malformation have been reported in embryonic and larval zebrafish Danio rerio exposed to nZnO (Zhu et al. 2009, Bai et al. 2010, Zhao et al. 2013. Although some studies found that toxicity of nZnO could be mainly attributed to dissolved Zn ions (Franklin et al. 2007, Wong et al. 2010, toxicity of nZnO cannot always be explained by the dissolved Zn ions (Bai et al. 2010.Particle-induced generation of reactive oxygen species (ROS) and dissolution to ionic Zn represent the primary modes of action for nZnO toxicity across all species tested (Ma et al. 2013). Indeed, ROS induction triggering an oxidative stress response has become a widely accepted paradigm for cellular effects of NPs (Nel et al. 2006, Zhu et al. 2009, Song et al. 2010, Sharma et al. 2012. Excessive production of ROS can induce pro-inflammatory and cytotoxic effects (Nel et al. 200...

show abstract

Comparative analysis of gene expression in brain, liver, skeletal muscles, and gills of zebrafish (Danio rerio) exposed to environmentally relevant waterborne uranium concentrations

Cited by 57 publications

References 38 publications

Effects of uranium on the metabolism of zebrafish, Danio rerio

Effects of uranium on the metabolism of zebrafish, Danio rerio

Relative importance of direct and trophic uranium exposures in the crayfish Orconectes limosus: Implication for predicting uranium bioaccumulation and its associated toxicity

Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles

Contact Info

Product

Resources

About