Comparative analysis of excretory–secretory products of muscle larvae of three isolates of Trichinella pseudospiralis by the iTRAQ method

Wang, Yang; Bai, Xue; Tang, Bin; Zhang, Yulu; Zhang, Lixiao; Cai, Xuepeng; Lin, Jiaojiao; Jia, Wan‐Zhong; Boireau, Pascal; Liu, Mingyuan

doi:10.1016/j.vetpar.2020.109119

Cited by 9 publications

(6 citation statements)

References 73 publications

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“…The FACS results showed that both MIF knockout and infection time affected the CD3 + CD8 − / CD3 + CD8 + ratio. Additionally, the ratio increased in both the WTs7 and KTs7 groups, which is similar to the results of a previous study, reflecting changes in the host immune response 75,76 . Increased IL‐4 and decreased IFN‐γ levels could be observed in the spleen of the WT and MIF KO mice at 14 and 35 dpi, except for the increase in IFN‐γ at 7 dpi.…”

Section: Discussionsupporting

confidence: 89%

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

Xie,

Xu,

Yan

et al. 2023

The FASEB Journal

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Trichinellosis caused by Trichinella spiralis (T. spiralis) is a major food‐borne parasitic zoonosis worldwide. Prevention of trichinellosis is an effective strategy to improve patient quality of life. Macrophage migration inhibitory factor (MIF) is closely related to the occurrence and development of several parasitic diseases. Studying the impact of MIF deficiency (Mif−/−) on the alterations in host fecal microbiota due to T. spiralis infection may contribute to proposing a novel dual therapeutic approach for trichinellosis. To reveal the diversity and differences in fecal microbial composition, feces were collected from T. spiralis‐uninfected and T. spiralis‐infected wild‐type (WT) and MIF knockout (KO) C57BL/6 mice at 0, 7, 14, and 35 days post‐infection (dpi), and the samples were sent for 16S rRNA amplicon sequencing on the Illumina NovaSeq platform. Flow cytometry was used to determine the expression levels of IFN‐γ and IL‐4 in the CD4+/CD8+ T‐cell sets of mouse spleens. The results showed that operational taxonomic unit (OTU) clustering, relative abundance of microbial composition, alpha diversity, and beta diversity exhibited significant changes among the eight groups. The LEfSe analysis selected several potential biomarkers at the genus or species level, including Akkermansia muciniphila, Lactobacillus murinus, Coprococcus catus, Firmicutes bacterium M10_2, Parabacteroides sp. CT06, and Bacteroides between the KTs and WTs groups. The predicted bacterial functions of the fecal microbiota were mainly involved in metabolism, such as the metabolism of carbohydrates, amino acids, energy, cofactors, vitamins, nucleotides, glycans, and lipids. Flow cytometry revealed an increased CD3+CD8−/CD3+CD8+ T‐cell ratio and increased IFN‐γ and IL‐4 levels in CD3+CD8− T‐cell sets from WT and MIF KO mice at 7 dpi. The results indicated that both MIF KO and infection time have a significant influence on the CD3+CD8−IFN‐γ+ and CD3+CD8−IL‐4+ response in mice after T. spiralis. In conclusion, this research showed alterations of the fecal microbiota and immune response in both WT and MIF KO mice before and after T. spiralis infection. These results revealed a potential role of MIF in regulating the pathogenesis of trichinellosis related to the intestinal microbiota. Importantly, the selected potential biomarkers combined with MIF will also offer a novel therapeutic approach to treat trichinellosis in the future.

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Section: Discussionsupporting

confidence: 89%

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

Xie,

Xu,

Yan

et al. 2023

The FASEB Journal

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“…ML were incubated at 37°C in 5% CO2. After 12 h, the culture medium containing ML excretory secretion products ( Ts -ML-ES) were centrifugated at 800×g for 15 min, followed by centrifugation at 5000×g for 15 min to remove ML [ 21 ]. The culture supernatant was then filtered through a 0.22 μm filter to remove debris, and the filtrate was concentrated using ultracentrifugation filters (Merck Millipore, USA) to a final volume of 150 mL.…”

Section: Methodsmentioning

confidence: 99%

Extracellular vesicles from Trichinella spiralis: Proteomic analysis and protective immunity

et al. 2022

Self Cite

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Trichinella spiralis (T. spiralis) derived extracellular vesicles (EVs) have been proposed to play a key role in regulating the host immune responses. In this study, we provided the first investigation of EVs proteomics released by T. spiralis muscle larvae (ML). T. spiralis ML EVs (Ts-ML-EVs) were successfully isolated and characterized by transmission electron microscopy (TEM) and western blotting. Using liquid chromatograph mass spectrometer (LC-MS/MS) analysis, we identified 753 proteins in the Ts-ML-EVs proteome and annotated by gene ontology (GO). These proteins were enriched in different categories by GO, kyoto encyclopedia of genes and genomes (KEGG) and domain analysis. GO enrichment analysis indicated association of protein deglutathionylation, lysosomal lumen and serine-type endopeptidase inhibitor activity with proteins which may be helpful during parasite-host interaction. Moreover, KEGG enrichment analysis revealed involvement of Ts-ML-EVs proteins in other glycan degradation, complement and coagulation cascades, proteasome and various metabolism pathways. In addition, BALB/c mice were immunized by subcutaneous injection of purified Ts-ML-EVs. Ts-ML-EVs group demonstrated a 23.4% reduction in adult worms and a 43.7% reduction in ML after parasite challenge. Cellular and humoral immune responses induced by Ts-ML-EVs were detected, including the levels of specific antibodies (IgG, IgM, IgE, IgG1 and IgG2a) as well as cytokines (IL-12, IFN-γ, IL-4 and IL-10) in serum. The results showed that Ts-ML-EVs could induce a Th1/Th2 mixed immune response with Th2 predominant. This study revealed a potential role of Ts-ML-EVs in T. spiralis biology, particularly in the interaction with host. This work provided a critical step to against T. spiralis infection based on Ts-ML-EVs.

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“…Trichinella species. Immunoproteomic studies of Trichinella spiralis , Trichinella pseudospiralis and Trichinella britovi have focused on ES proteins [ 51 , 52 , 53 , 54 , 55 ], with the intention of improving the immuno- or sero-diagnosis of trichinellosis. Numerous immunogenic proteins, including serine protease, enolase and peptidase inhibitors, were recognised by antibodies in the sera from patients or from experimental animals.…”

Section: Recent High-throughput Proteomic Explorations Of Parasitic Nematodesmentioning

confidence: 99%

Prospects of Using High-Throughput Proteomics to Underpin the Discovery of Animal Host–Nematode Interactions

Wang

Gasser

2021

Pathogens

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Parasitic nematodes impose a significant public health burden, and cause major economic losses to agriculture worldwide. Due to the widespread of anthelmintic resistance and lack of effective vaccines for most nematode species, there is an urgent need to discover novel therapeutic and vaccine targets, informed through an understanding of host–parasite interactions. Proteomics, underpinned by genomics, enables the global characterisation proteins expressed in a particular cell type, tissue and organism, and provides a key to insights at the host–parasite interface using advanced high-throughput mass spectrometry-based proteomic technologies. Here, we (i) review current mass-spectrometry-based proteomic methods, with an emphasis on a high-throughput ‘bottom-up’ approach; (ii) summarise recent progress in the proteomics of parasitic nematodes of animals, with a focus on molecules inferred to be involved in host–parasite interactions; and (iii) discuss future research directions that could enhance our knowledge and understanding of the molecular interplay between nematodes and host animals, in order to work toward new, improved methods for the treatment, diagnosis and control of nematodiases.

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Comparative analysis of excretory–secretory products of muscle larvae of three isolates of Trichinella pseudospiralis by the iTRAQ method

Cited by 9 publications

References 73 publications

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

Extracellular vesicles from Trichinella spiralis: Proteomic analysis and protective immunity

Prospects of Using High-Throughput Proteomics to Underpin the Discovery of Animal Host–Nematode Interactions

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