Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.)

Gao, Wenrui; Wang, Xiansheng; Liu, Qingyou; Peng, Hui; Chen, Chen; Li, Jiangui; Jusong, Zhang; Hu, Songnian; Ma, Hao

doi:10.1016/j.bbrc.2008.09.030

Cited by 54 publications

(34 citation statements)

References 29 publications

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“…In Arabidopsis, HsfB1 and HsfB2b have been found to be transcription repressors and negatively regulate the expression of heat-inducible HSFs (HsfA2, HsfA7a, HsfB1, and HsfB2b) and several heat shock protein genes (Ikeda et al 2011); in Arabidopsis single and double mutants of HsfB1 and HsfB2b, expression of Pdf1.2a/b is significantly increased (Kumar et al 2009). Chickpea (Cicer arietinum L.) is the third important legume crop in terms of cultivation area, mainly grown in the arid and semi-arid regions, such as Central and South Asia, Southern Europe, Northern and Eastern Africa, Australia, and America (Gao et al 2008). Due to long-term evolution and adaptation to extreme conditions, chickpea has been found rich in resistance genes for a range of abiotic stresses such as drought and cold, and has been suggested as a model plant for investigation of physiological mechanisms of plant development and responses to stresses (Singh et al 1998).…”

Section: Introductionmentioning

confidence: 99%

CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

Wang

Yang

et al. 2015

Plant Mol Biol Rep

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Heat shock transcription factor (HSF) plays an essential role on the increased tolerance against heat stress by regulating the expression of heat-responsive genes. In this study, a HSF gene, CarHSFB2, was isolated and characterized in chickpea. CarHSFB2 was a nuclear protein with a predicted polypeptide of 267 amino acids and encoded by a single/low copy genes. Phylogenetic analysis showed that CarHSFB2 belonged to the class B HSFs. It had little or no any transcription activation activity due to lack of aromatic, hydrophobic, and acidic amino acid (AHA) motifs. CarHSFB2 showed different expression patterns among different developmental processes (leaf senescence, developing seed, and embryo of germinating seed). It was induced by the stress of heat, salt, wound and drought, and the treatment of H 2 O 2 , IAA, and GA3, respectively, while inhibited by 6-BA. However, the other stress and chemical treatments (cold, ABA, MeJA, Et, and SA) had no obvious effect on its expression. Overexpression of CarHSFB2 in Arabidopsis seedlings showed the increased tolerance to drought and heat stress. Additionally, stress-responsive genes, RD22, RD26, and RD29A, showed significantly higher expression levels in transgenic Arabidopsis seedlings than in the wild type (WT) under drought stress, whereas HsfA2, HsfB2a, and HsfA7a in transgenic Arabidopsis seedlings were markedly accumulated in transcript level than in the WT under heat stress. All these results indicate that CarHSFB2, a class B HSF, positively functions in different developmental processes and various stress responses, especially in positive response to heat and drought stresses, in chickpea.

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Section: Introductionmentioning

confidence: 99%

CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

Wang

Yang

et al. 2015

Plant Mol Biol Rep

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“…Recently, a BAC/binary BAC-based physical map of cultivated chickpea (cv Hadas) has also been developed . In addition, a few EST projects have provided a few thousand singlepass sequences (Buhariwalla et al, 2005;Gao et al, 2008;Ashraf et al, 2009;Varshney et al, 2009;Jain and Chattopadhyay, 2010). Although several genes/ESTs involved in various stress responses have been identified based on transcriptomic and proteomic studies (Pandey et al, 2006(Pandey et al, , 2008Mantri et al, 2007;Molina et al, 2008Molina et al, , 2011Varshney et al, 2009), the gene discovery has been very limited in chickpea.…”

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confidence: 99%

Gene Discovery and Tissue-Specific Transcriptome Analysis in Chickpea with Massively Parallel Pyrosequencing and Web Resource Development

et al. 2011

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Chickpea (Cicer arietinum) is an important food legume crop but lags in the availability of genomic resources. In this study, we have generated about 2 million high-quality sequences of average length of 372 bp using pyrosequencing technology. The optimization of de novo assembly clearly indicated that hybrid assembly of long-read and short-read primary assemblies gave better results. The hybrid assembly generated a set of 34,760 transcripts with an average length of 1,020 bp representing about 4.8% (35.5 Mb) of the total chickpea genome. We identified more than 4,000 simple sequence repeats, which can be developed as functional molecular markers in chickpea. Putative function and Gene Ontology terms were assigned to at least 73.2% and 71.0% of chickpea transcripts, respectively. We have also identified several chickpea transcripts that showed tissue-specific expression and validated the results using real-time polymerase chain reaction analysis. Based on sequence comparison with other species within the plant kingdom, we identified two sets of lineage-specific genes, including those conserved in the Fabaceae family (legume specific) and those lacking significant similarity with any non chickpea species (chickpea specific). Finally, we have developed a Web resource, Chickpea Transcriptome Database, which provides public access to the data and results reported in this study. The strategy for optimization of de novo assembly presented here may further facilitate the transcriptome sequencing and characterization in other organisms. Most importantly, the data and results reported in this study will help to accelerate research in various areas of genomics and implementing breeding programs in chickpea.

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“…Recently several EST sequencing projects have led to generation of large scale EST sequences through single pass sequencing (Varshney et al 2009 ;Gao et al 2008 ;Ashraf et al 2009 ;Jain and Chattopadhyay 2010 ).…”

Section: Functional Markers Ests Bac Librariesmentioning

confidence: 99%

Advances in Chickpea Genomics

Gaur

Thudi

Srinivasan

et al. 2013

Legumes in the Omic Era

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Chickpea ( Cicer arietinum L.), the second largest consumed pulse crop of the world after common bean, is grown in over 50 countries and traded across 140 countries. After several decades of slow progress, the recent years have witnessed spectacular progress in development of genetic (mapping populations) and genomic resources (structural and functional molecular markers, integrated genetic map and mapping of genes/quantitative trait loci; QTLs) in this crop. QTLs associated with traits of interest including resistance against wilt, Ascochyta blight, Botrytis grey mould and rust; tolerance against salinity and drought and agronomic traits have been identifi ed and validated. A more than 30 genetic linkage maps available in this crop are useful resources for genetic analysis and marker assisted breeding. Genomic tools like bacterial artifi cial chromosome (BAC) libraries, expressed sequence tags (ESTs) and targeting induced local lesions in genome (TILLING) mutants have been developed in chickpea to facilitate the genome sequencing efforts in this crop. A major landmark in chickpea genomics has been the publication of 738 Mb draft whole genome sequence assembly of a kabuli variety, CDC Frontier. Now, chickpea is one of the most advanced grain legumes in terms of availability of genomic resources. Efforts have already begun on application of these genomics resources in chickpea improvement. This book chapter provides an update on the development of genetic and genomic resources for chickpea and their current and potential uses in chickpea improvement.

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Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.)

Cited by 54 publications

References 29 publications

CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

Gene Discovery and Tissue-Specific Transcriptome Analysis in Chickpea with Massively Parallel Pyrosequencing and Web Resource Development

Advances in Chickpea Genomics

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