Comparative analysis of Epstein–Barr virus (EBV) detection by nested-PCR and non-isotopic in situ hybridization in nasopharyngeal carcinoma (NPC)

Vera-Sempere, Francisco; Botella, Maria-Soledad; Morera, Constantino

doi:10.1016/s0009-8981(97)00209-x

Cited by 14 publications

(15 citation statements)

References 29 publications

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“…Although this variability might have epidemiological causes, it seems to be more related to technical considerations. 2,7,9,22,23 Latent membrane protein-I is the only EBV oncogene capable of transforming rodent fibroblasts in vitro, and it is essential for primary B-Iymphocyte transformation. 1,2 Few studies have found a prognostic relationship between LMP-l expression and NPC.…”

Section: Introductionmentioning

confidence: 99%

Association of Epstein-Barr Virus and Nasopharyngeal Carcinoma in Caucasian Patients

Plaza¹,

Manzanal²,

Fogué³

et al. 2002

Ann Otol Rhinol Laryngol

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We evaluated the association of nasopharyngeal carcinoma (NPC) and Epstein-Barr virus (EBV) in Spanish patients, and studied the expression of EBV products (latent membrane protein-1 [LMP-1] and ZEBRA proteins) by NPC cells and its possible prognostic value. In situ hybridization (ISH) for EBV-encoded nonpolyadenylated RNAs (EBERs) and immunohistochemical expression of LMP-1 and ZEBRA proteins by immunohistochemistry were examined in formalin-fixed, paraffin-embedded NPC specimens from 30 patients, and a survival analysis was done by the Kaplan-Meier method. We detected EBERs by ISH in 96.67% of the NPC cases, and detected expression of LMP-I in 43.33% of the NPC cases and expression of ZEBRA protein in 6.67% of the NPC cases. We conclude that ISH for expression of EBERs is an adequate method for detection of EBV in NPC. LMP-1 is not frequently expressed in NPC cells (43.33%). Most NPC cells carry a latent EBV infection. LMP-1 expression might have worsened the prognosis of NPC in our series.

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Section: Introductionmentioning

confidence: 99%

Association of Epstein-Barr Virus and Nasopharyngeal Carcinoma in Caucasian Patients

Plaza¹,

Manzanal²,

Fogué³

et al. 2002

Ann Otol Rhinol Laryngol

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“…This is in accordance with international literature, where 75-100% of cases of non-keratinizing NPC show positive association with EBV. The presence of negative cases might be related to the presence of smaller number of viral copies [36]. In eight out of 39 cases we performed In situ hybridization-EBER on the primary tumor and lymphnode metastasis and both of them were positive for EBV-EBER.…”

Section: Discussionmentioning

confidence: 99%

The incidence of Epstein–Barr virus in nasopharyngeal carcinoma of Jordanian patients

Matalka

Hamad

Al-Hussaini

et al. 2011

Eur Arch Otorhinolaryngol

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Aim of this study was to investigate the incidence of Epstein-Barr virus (EBV) in patients diagnosed with undifferentiated nasopharyngeal carcinoma (UNPC) from the Northern Province of Jordan. All cases diagnosed with UNPC at King Abdullah University Hospital, Irbid, Jordan, between the years 1991 and 2009 inclusive were examined. Clinical data including age, gender, mode of presentation, site of biopsy were retrieved from pathology reports. In situ hybridization for (EBV)--EBERs was performed on cases with available paraffin blocks. Correlation between the different clinical variables and results of in situ hybridization was performed. There were 49 cases diagnosed with UNPC, only 39 specimens were available and studied. The median age of presentation was 41 years (range 9-70 years). Bimodal age distribution was noted, the first peak between 15 and 19 years of age and second between 60 and 64 years of age. Males were slightly more commonly affected than females. Cervical lymph node enlargement was the most common mode of presentation, followed by nasal obstruction. Biopsies were obtained primarily from the posterior nasal space, followed by cervical lymph node. Positive staining for EBERs by in situ hybridization was seen in 92.3% of the cases examined. There was no difference in detection rate between males and females or adults and pediatrics. All cases obtained from posterior nasal space were positive. The three negative cases were from biopsies obtained from cervical lymph nodes, which was statistically significant (P value <0.05). Nasopharyngeal carcinoma in Jordan is seen in both children and adults. It is associated with EBV infection in most, but not all cases. Posterior nasal space shows a more consistent staining for EBERs than cervical lymph nodes. The presence of other association with UNPC including cigarette smoking could possibly explain the cases with negative association.

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“…TSA-IHC is thus very well suited for fast and reliable examination of EBV involvement in nasopharyngeal tumors, since it allows detection of low-abundance expression of the viral oncoprotein in situ [42]. Evaluation based on direct comparison of RT-PCR and TSA-IHC would help to prove sensitivity and should be part of further investigations on LMP-1 [43]. Our data derived from conventional immunohistochemistry surprisingly showed a trend towards better overall survival for LMP-1-negative tumors.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Impact of EBV-Related LMP-1, Histologic Type, and Environmental Factors in Nasopharyngeal Carcinoma in a German Population

et al. 2004

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Introduction: This retrospective study addressed the possible involvement of latent Epstein-Barr virus (EBV) infection, in particular LMP-1 expression, and further exogenous factors, i.e. tobacco, alcohol and occupational hazardous substances, in nasopharyngeal carcinoma (NPC) in a German population. Patients and Methods: From 1980 to 2000, 44 patients suffering from histologically confirmed NPC were entered into the study. 33 specimens were available for immunostaining (IHC) to analyze LMP-1 expression. Information about environmental exposures were obtained employing a detailed standardized questionnaire. Results: Outcome of patients with squamous cell NPC (SC-NPC) was significant worse than that of those with non-keratinizing NPC (NK-NPC). Age and tumor size correlated with response to therapy. The group with negative conventional LMP-1 staining showed better overall survival after 5 years compared to the group with positive or marginally positive LMP-1 detection (not significant). Nevertheless, after staining by tyramid- augmented IHC (TSA-IHC), nearly all specimens with negative LMP-1-staining in conventional IHC were found to be clearly positive. All patients with SC-NPC were smokers. The distribution of smokers and non-smokers in the group of NK-NPC was balanced. Comparable to the tobacco observation, there was also a correlation between high alcohol consumption and SC-NPC. Conclusion: Prognosis of NPC is mainly dependent on histologic type. Prognostic impact of LMP-1 is still unclear since LMP-1 was detected in all specimens using TSA-IHC. Therefore, TSA-IHC-LMP-1 detection might be interesting for diagnostic specification and development of new therapeutic strategies in NPC.

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Comparative analysis of Epstein–Barr virus (EBV) detection by nested-PCR and non-isotopic in situ hybridization in nasopharyngeal carcinoma (NPC)

Cited by 14 publications

References 29 publications

Association of Epstein-Barr Virus and Nasopharyngeal Carcinoma in Caucasian Patients

Association of Epstein-Barr Virus and Nasopharyngeal Carcinoma in Caucasian Patients

The incidence of Epstein–Barr virus in nasopharyngeal carcinoma of Jordanian patients

Prognostic Impact of EBV-Related LMP-1, Histologic Type, and Environmental Factors in Nasopharyngeal Carcinoma in a German Population

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