Comparative analysis of eight esterification methods in the quantitative determination of vegetable oil fatty acid methyl esters (FAME)

Milinsk, Maria Cristina; Matsushita, Makoto; Visentainer, Jesuí V.; Oliveira, Celso Maran de; Souza, Nilson Evelázio de

doi:10.1590/s0103-50532008000800006

Cited by 71 publications

(55 citation statements)

References 13 publications

(18 reference statements)

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“…The n-6/n-3 ratios of breaded chicken steak were between 22.87 and 38.90, which are much higher than the maximum limit of 4.0 recommended by Enser et al (1998). The high n-6/n-3 ratios are related to the high linoleic acid (LA) contents of the n-6 series derived from the soybean oil (MILINSK et al, 2008;MARTIN et al, 2008) used in the industrial pre-frying step. During home preparation, breaded chicken steak is generally fried, which further increases the LA amount.…”

Section: Fatty Acid Methyl Estersmentioning

confidence: 97%

See 1 more Smart Citation

Proximate composition and quantification of fatty acids in breaded chicken steak

Tanamati¹,

Aguiar²,

Boroski³

et al. 2011

Ciênc. Tecnol. Aliment.

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Section: Fatty Acid Methyl Estersmentioning

confidence: 97%

“…Changes in human eating habits have required new industrialized food products to meet the demand of quick home preparation foods (NEVES; CHADDAD; LAZZARINI, 2002). Restructured breaded steak stands out for its easy preparation and as an accessible protein source.…”

Section: Introductionmentioning

confidence: 99%

Proximate composition and quantification of fatty acids in breaded chicken steak

Tanamati¹,

Aguiar²,

Boroski³

et al. 2011

Ciênc. Tecnol. Aliment.

Self Cite

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“…3, 2010 reagent, incomplete extraction of FAMEs, and the loss of very volatile short-chain FAMEs. 4 Thus, the search for methods that minimize such interferences is warranted.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 The most used derivatization processes in the analysis of fatty acids involve acid and basic catalysis. 4 Basic catalysis with either NaOCH 3 or KOH in methanol at room temperature is considered the most reliable technique in the identification of fatty acid conjugated isomers, such as linoleic acid, as it does not produce either isomerization or artifacts. Furthermore, it reduces the loss of short-chain fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Validation of the determination of fatty acids in milk by gas chromatography

Simionato¹,

Garcia²,

Santos³

et al. 2010

J. Braz. Chem. Soc.

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Foram analisados os ésteres metílicos de ácidos graxos (FAMEs) em amostras comerciais de leite empregando cromatografia gasosa acoplada ao detector por ionização em chama. Os ácidos graxos saturados (SFA) foram os mais abundantes. Os principais SFA encontrados foram o ácido palmítico (16:0), o ácido esteárico (18:0) e o ácido mirístico (14:0). Foram obtidas diferenças significativas (P < 0,500) entre as quantidades de ácido palmítico (276 ± 17 mg g -1 e 248 ± 20 mg g -1 ) e ácido mirístico (95 ± 5 mg g -1 e 85 ± 7 mg g -1 ) nas amostras analisadas. Entretanto, não foi observada uma diferença significativa para o ácido esteárico (113 ± 6 mg g -1 e 114 ± 11 mg g -1 ). O isômero conjugado do ácido linoleico (CLA) 18:2c9t11 foi detectado nas amostras e quantificado. Para este ácido, as diferenças encontradas nas amostras analisadas não foram significativas (10,4 ± 0,7 mg g -1 e 9,9 ± 0,6 mg g -1 ). A análise de uma amostra do material de referência certificado de leite (RM-8435 NIST) mostrou uma boa recuperação (> 80%) indicando que o método pode ser aplicado para determinar eficientemente ácidos graxos em amostras de leite e em produtos lácteos.Fatty acid methyl esters (FAMEs) in commercial milk samples were analyzed by gas chromatography coupled with flame ionization detection. The saturated fatty acids (SFA) were the most abundant. The major SFA were palmitic acid (16:0), estearic acid (18:0), and myristic acid (14:0). Significant differences (P < 0.500) were found between the amounts of palmitic acid (276 ± 17 mg g -1 and 248 ± 20 mg g -1 ) and myristic acid (95 ± 5 mg g -1 and 85 ± 7 mg g -1 ) in samples. However, no difference was observed for estearic acid (113 ± 6 mg g -1 and 114 ± 11 mg g -1 ). The conjugated linoleic acid (CLA) isomer 18:2c9t11 was detected in the samples and quantified. However, the differences found between the samples analyzed were not significant (10.4 ± 0.7 mg g -1 and 9.9 ± 0.6 mg g -1 ). The analysis of a certified reference powder milk sample (RM-8435 NIST) gave good recoveries (> 80%), indicating that the method can efficiently determine fatty acids in milk and dairy products.

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“…16 Biodiesel must be in accordance with a set of quality parameters to be employed, that includes purity and impurities contents; performance characteristics, as cetane number, oxidative stability; and physical properties, as viscosity, and specific mass. 19 The purity of biodiesel is expressed as esters content, and can be estimated by several instrumental techniques, as gas chromatography-flame ionization detector (GC-FID), [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] gas chromatography-mass selective detector (GC-MSD), 22,38,[40][41][42][43][44][45][46] high performance liquid chromatography--evaporative light scattering detector (HPLC-ELSD), [22][23][24][25]47,48 high performance liquid chromatography-refractive index detector (HPLC-RID), 49 high performance liquid chromatography-variable wavelength detector (HPLC-VWD), 23,32,34 gel permeation chromatography (GPC), 23 high performance thin layer chromatography (HPTLC), 50 nuclear magnetic resonance (NMR), …”

Section: Introductionmentioning

confidence: 99%

GC-FID methodology validation for the fatty esters content determination in biodiesel with hexadecyl acetate as the internal standard

et al. 2017

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Biodiesel purity expressed as fatty esters content is one of its most important quality parameters. Although several instrumental methods have been employed, gas chromatography with internal standard calibration has been the most used. Biodiesel is a very complex matrix, therefore finding a suitable internal standard (IS) is not straightforward. EN14103:2003 standard established methyl heptadecanoate (C17:0) as IS. Since this fatty ester is naturally present in some biodiesel feedstock as tallow, that standard was reviewed in 2011, and methyl nonadecanoate (C19:0) became the new IS. In turn, C19:0 produces a peak on the chromatogram that is difficult to resolve from methyl linoeate (C18:2) and metyl linolenate (C18:3) ones. In this paper, a GC-FID method for the determination of the esters content in methyl and ethyl biodiesel from canola and soy oils, using hexadecyl acetate as a new internal standard was validated. EN14103:2003 was used as reference method. Method selectivity, accuracy (t student parameter < 2.18 from both methods), repeatability (0.1 -0.4%), intermediate precision (0.2 -1.8%), and robustness (0.1 -1.6%) were investigated and considered appropriate for the scope.

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Comparative analysis of eight esterification methods in the quantitative determination of vegetable oil fatty acid methyl esters (FAME)

Cited by 71 publications

References 13 publications

Proximate composition and quantification of fatty acids in breaded chicken steak

Proximate composition and quantification of fatty acids in breaded chicken steak

Validation of the determination of fatty acids in milk by gas chromatography

GC-FID methodology validation for the fatty esters content determination in biodiesel with hexadecyl acetate as the internal standard

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