Comparative analysis of different DNA barcodes for applications in the identification and production of Pyropia

Gu, Ziqiang; Yang, Lintao; Chen, Zepan; Chen, Weizhou

doi:10.1016/j.algal.2020.101874

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…Hence there is an urgency in evaluating a universal barcode gene for all 3 phyla (Phaeophyta , Rhodophyta and Chlorophyta) of seaweeds. rbcL barcodes has proven to be a potential barcode gene for identification of natural, aquacultured and processed seaweeds Saunders and Moore, 2013;Gu et al, 2020). rbcL gene has also proven its ability in unmasking overlooked seaweed diversity (Saunders and McDevit, 2013), identification of cryptic invasive (Saunders, 2009; and exotic (Mineur et al, 2012;Montes et al, 2017) species, besides discovering new seaweed species (Griffith et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Identifying seaweeds species of Chlorophyta, Phaeophyta and Rhodophyta using DNA barcodes

Prasanthi

PrasannaKumar

Annadurai

et al. 2020

Preprint

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Strengthening the DNA barcode database is important for a species level identification, which was lacking for seaweeds. We made an effort to collect and barcode seaweeds occurring along Southeast coast of India. We barcoded 31 seaweeds species belonging to 21 genera, 14 family, 12 order of 3 phyla (viz., Chlorophyta, Ochrophyta and Rhodophyta). We found 10 species in 3 phyla and 2 genera (Anthophycus and Chnoospora) of Ochrophyta were barcoded for the first time. Uncorrected p-distance calculated using K2P, nucleotide diversity and Tajima’s test statistics reveals highest values among the species of Chlorophyta. Over all K2P distance was 0.36. The present study revealed the potentiality of rbcL gene sequences in identification of all 3 phyla of seaweeds. We also found that the present barcode reference libraries (GenBank and BOLD) were insufficient in seaweeds identification and more efforts were needed for strengthening local seaweed barcode library to benefit rapids developing field such as environmental DNA barcoding. We also show that the constructed barcode library could aid various industrial experts involved in seaweed bio-resource exploration and taxonomy/non-taxonomic researches involved in climate, agriculture and epigenetics research in precise seaweed identification. Since the rise of modern high-throughput sequencing technologies is significantly altering bio-monitoring applications and surveys, reference datasets such as ours will become essential in ecosystem’s health assessment and monitoring.

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