Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton

Abstract

Promoters are specified segments of DNA that lead to the initiation of transcription of a specific gene. The designing of a gene cassette for plant transformation is significantly dependent upon the specificity of a promoter. Constitutive Cauliflower mosaic virus promoter, CaMV35S, due to its developmental role, is the most commonly used promoter in plant transformation. While Gossypium hirsutum (Gh) being fiber-specific promoter (GhSCFP) specifically activates transcription in seed coat and fiber associated genes. The Expansin genes are renowned for their versatile roles in plant growth. The overexpression of Expansin genes has been reported to enhance fiber length and fineness. Thus, in this study, a local Cotton variety was transformed with Expansin (CpEXPA1) gene, in the form of two separate cassettes, each with a different promoter, named as 35SEXPA1 and FSEXPA1 expressed under CaMV35S and GhSCFP promoters respectively. Integration and Spatiotemporal relative expression of the transgene were studied in an advanced generation. GhSCFP bearing transgene expression was significantly higher in Cotton fiber than other plant parts. While transgene with CaMV35S promoter was found to be continually expressing in all tissues but the expression was lower in fiber than that expressed under GhSCFP. The temporal expression profile was quite interesting with a gradual increasing pattern of both constructs from 1DPA (days post anthesis) to 18DPA and decreased expression from 24 to 30 DPA. Besides the relative expression of promoters, fiber cellulose quantification and fluorescence intensity were also observed. The study significantly compared the two most commonly used promoters and it is deduced from the results that the GhSCFP promoter could be used more efficiently in fiber when compared with CaMV35S which being constitutive in nature preferred for expression in all parts of the plant.

Impact of Lycopene Supplemented Canola Meal-Based Diet on Growth Performance, Nutrient Digestibility and Antioxidant Status of Catla Catla Fingerlings

“…We compared the information obtained from metaphase FISH (Mahmood-ur-Rahman et al, 2012;Latif et al, 2015;Ali et al, 2016) to better explain the phenomenon of position effect and effect of transgene copy number. Our results are very similar to already published data (Rao et al, 2013;Ali et al, 2016;Yaqoob et al, 2020) and confirm the idea of copy number variation. There are lots of techniques which are developed to visualize the single copy of foreign DNA in plant species.…”

Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton

“…Different plant expression vectors are used for plant transformation under efficient promoters. Promoter is a specified Segment of DNA that initiates a transcription of a specific gene (Yaqoob et al, 2020). Promoters are selected or designed based on the expression of the required gene.…”

“…This has been proved through various studies that revealed the relative expression of the tissue specific and constitutive promoters. The level of expression of the gene in cotton plant indicates the difference between constitutive and tissue specific promoter (Yaqoob et al, 2020). The introduction of gene for overexpression in cotton using CaMV35S promoter causes visible differences in plant phenotypes such as plant height, boll number, boll weight, fiber strength and length .…”

Management of Ochratoxigenic Fungi by Phytochemicals of Amaranthus Viridis L.

“…Very recently, several studies have been conducted to detect foreign genes by using FISH e.g. CRSP-1 and CRSP-2 (Ali et al, 2016), phytochrome B gene (Rao et al, 2013) and CpEXPA1 gene (Yaqoob et al, 2020) in cotton.…”