Comparative analysis of combined phosphorus and drought stress-responses in two winter wheat

Zhang, Xiangchi; Lu, Weidan; Wang, Xiaoli; Ma, Bin; Fu, Kaiyong; Li, Chunyan; Li, Cheng

doi:10.21203/rs.3.rs-45684/v1

Cited by 3 publications

(6 citation statements)

References 54 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It probably suggested that the root starch begin to degrade and the root tissue is already in a state of dehydration, which was more serious under LP than CP treatment. This speculation were in agreement with the performance of DNA fragmentation and root water content [42].…”

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelssupporting

confidence: 89%

“…According to the results of gene chip in our laboratory, compared with CP, the gene of β-amylase involved in starch degradation was signi cantly up-regulated. While, the genes of coding starch branching enzyme III and glycogen (starch) synthase related to starch synthesas were signi cantly down-regulated at D7 in LP treatment [42]. Based on this, we speculated that the degradation rate of starch was stimulated and the synthesis rate of starch decreased under LP treatment.…”

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelsmentioning

confidence: 88%

“…With the increase of lateral root growth, the response of genes related to cell wall synthesis and growth to phosphorus was accelerated. For instance, the expression of the gene coding cell wall invertase was signi cantly increased [42].…”

Section: Discussionmentioning

confidence: 99%

“…However, in this study, compared with CP, the expression of PT1 gene (high-a nity phosphate transporter) in the roots cultured by LP was signi cantly down-regulated, and the gene of phosphate transporter 8 was signi cantly up-regulated at D7. Among three MYB-related protein genes induced by low phosphorus, two were downregulated and one was up-regulated [42]. In addition, most of the differential metabolites involved in sugar metabolism showed a response ratio lower than 1, indicating a decrease in phosphorus-de cient roots (Table 4).…”

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelsmentioning

confidence: 99%

See 3 more Smart Citations

Ionomic and metabolic responses to drought stress in elite wheat seedlings under two phosphorus levels

Zhang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background Wheat (Triticum aestivum L.) is the main food crop in the worldwide. Low soil phosphorus levels and drought conditions are important constraints for wheat production in most of the areas where wheat is grown. In this study, the analysis of mineral elements and metabolites were used to investigate the ionomic and metabolic responses to drought stress of wheat plants cultured by low phosphorus (LP) and conventional phosphorus (CP) supply, respectively. Results We found that the wheat plants subjected to LP treatment had denser roots, and the total root volume was significantly higher than that under the CP treatment. The roots cultured by two phosphorus levels underwent the process of programmed cell death under drought stress, however, the genomic DNA degradation level of the roots in LP was significantly weaker than that in CP after rehydration for 3d. The analysis of mineral elements and metabolites showed that CP treatment was more sensitive to drought stress, and drought stress had more influence on the shoots of CP treatment than the roots. While the effect of drought stress on LP treatment roots were greater than that on shoots. With the extension of drought stress, the effect on sugar metabolism was greater. Conclusions The wheat plants under LP treatment was more adaptive to drought stress than that in CP treatment, which probably is based on the presence of the comprehensive mobilization of sugar metabolism responsible for the regulation of osmotic balance, as well as the accumulation of various organic acids responsible for the maintenance of the intracellular ion homeostasis.

show abstract

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelssupporting

confidence: 89%

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Metabolic Changes In Response To Drought Stress Under Two Phosphorus Levelsmentioning

confidence: 99%

See 2 more Smart Citations

Ionomic and metabolic responses to drought stress in elite wheat seedlings under two phosphorus levels

Zhang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This may be due to that with the growth of lateral roots, the responses of cell wall synthesis and growth-related genes to P accelerate. For example, the gene chip detection in our previous study found that the expression of the gene encoding cell wall invertase in wheat root treated with LP and 7 days of drought stress increased significantly [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Ionomic and metabolic responses of wheat seedlings to PEG-6000-simulated drought stress under two phosphorus levels

Zhang

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Background Wheat (Triticum aestivum L.) is a major food crop worldwide. Low soil phosphorus content and drought are the main constraints on wheat production in Xinjiang, China. Methods In this study, the ionic and metabolic responses of one wheat variety (“Xindong20”) to drought stress simulated by using polyethylene glycol 6000 (PEG-6000) were investigated under low phosphorus (LP) and conventional phosphorus (CP) conditions by analysing wheat mineral elements and metabolites. Besides, due to xanthohumol was the metabolite with the most significant difference in expression detected in “Xindong 20”, two wheat variety “Xindong20 and Xindong 23” were selected to conduct the germination test simultaneously, to further verify the function of xanthohumol in wheat growth. Xanthohumol was mixed with PEG solution (20%) to prepare PEG solutions with different concentrations (0%, 0.1%, 0.5%, and 1%) of xanthohumol. Then wheat grains were soaked in the solutions for 20 hours, followed by a germination test. After 7 days, the indicators including shoot length, max root length, and root number were determined to identify whether the metabolite was beneficial to improve the drought tolerance of wheat. Results The results showed that the root density and volume of wheat in LP treatment were higher than those in CP treatment. The roots underwent programmed cell death both in LP and CP treatments under PEG-6000-simulated drought stress, however, the DNA degradation in root cells in LP treatment was lower than that in CP treatment after rehydration for 3 d. Before drought stress, the malondialdehyde (MDA) content in shoot and the peroxidase (POD) activity in root in LP treatment were significantly higher than those in CP treatment, while the soluble sugar content and chlorophyll content in LP treatment were significantly lower than those in CP treatment. During drought stress, the POD activity maintained at a high level and the soluble sugar content gradually increased in LP treatment. After rehydration, the MDA content still maintained at a high level in LP treatment, the superoxide dismutase (SOD) activity increased, and the contents of soluble sugar and chlorophyll were significantly higher than those in CP treatment. The analysis of mineral elements and metabolites showed that the wheat in CP treatment was more sensitive to drought stress than that in LP treatment. Besides, the effect of drought stress was greater on shoot than on root in CP treatment, while it was opposite in LP treatment. The effect of drought stress on sugar metabolism gradually increased. Germination assays showed that 0.1% exogenous xanthohumol addition could significantly increase the shoot length of the two wheat varieties under drought stress. Conclusion Appropriate low phosphorus supply could increase antioxidant enzyme activity in wheat, and enhance sugar metabolism to regulate osmotic balance, as well as the accumulation of various organic acids to maintain the intracellular ion homeostasis. Therefore, compared to the conventional phosphorus supply level, appropriate low phosphorus supply can significantly improve the drought tolerance of wheat. Additionally, addition of 0.1% exogenous xanthohumol, an important differential expressed metabolite in drought-stressed wheat, could effectively promote wheat shoot growth under drought stress.

show abstract

A protective foliar complex boosts sugarcane quality and energy yield by reducing oxidative stress under drought

Jacomassi,

Momesso,

Pacola

et al. 2023

Crop Science

View full text Add to dashboard Cite

Water stress negatively impacts plant morphology and physiology by inducing metabolic oxidative stress, which leads to cellular damage. Applying nutrients and/or organic molecules, such as amino acids, may mitigate the effects of drought stress. In the present study, the effects of a protective foliar complex (PFC) on late‐harvest sugarcane (Saccharum spp L.) (dry season) were evaluated. Four field experiments were carried out in commercial areas of sugarcane production in the central‐south region of Brazil using sugarcane variety CTC4: in the fourth and second ratoons in 2019 and in the fifth and third ratoons in 2020. The treatments consisted of foliar application or no application of the PFC at the beginning of the dry period in June. The PFC was applied at a dose of 10 L ha−1 in a water volume of 100 L ha−1. PFC application increased the activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) in sugarcane leaves. PFC application also reduced leaf levels of malondialdehyde and hydrogen peroxide (H2O2). On average, PFC application increased sucrose accumulation by 2.26 and 1.74 Mg ha−1 in 2019 and 2020, respectively, indicating improved raw material quality. In addition, PFC application mitigated damage caused by drought, with average increases in stalk productivity of 10.7 and 7.6 Mg ha−1 in 2019 and 2020, respectively. The positive effects of PFC application on plant antioxidant enzymes enabled greater biomass accumulation and, in turn, higher energy cogeneration.

show abstract

Comparative analysis of combined phosphorus and drought stress-responses in two winter wheat

Cited by 3 publications

References 54 publications

Ionomic and metabolic responses to drought stress in elite wheat seedlings under two phosphorus levels

Ionomic and metabolic responses to drought stress in elite wheat seedlings under two phosphorus levels

Ionomic and metabolic responses of wheat seedlings to PEG-6000-simulated drought stress under two phosphorus levels

A protective foliar complex boosts sugarcane quality and energy yield by reducing oxidative stress under drought

Contact Info

Product

Resources

About