Comparative analysis of chemical constituents by HPLC–ESI–MSⁿ and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale

Abstract

Dendrobium huoshanense is a Chinese medicinal herb that has high quality and excellent efficacy. However, the chemical basis of its activity is still unclear. Of note, Dendrobium officinale is the most widely utilized among the Dendrobium species. Therefore, the current study systematically investigated the chemical constituents of methanolic extracts and different polar fractions of aqueous extracts from the two herbs by HPLC–ESI–MSn, and then compared in vitro antioxidant activities of their five different polar extracts. Consequently, 61 and 49 compounds were identified from D. huoshanense and D. officinale, respectively, of which 43 compounds were common to both species. In addition, 17 out of 22 different compounds were identified only in D. huoshanense. Moreover, the peak areas of some shared identical compounds of D. huoshanense were significantly larger than that of D. officinale. In vitro antioxidant evaluation results showed that the n‐BuOH‐soluble fraction of the two herbs exhibited remarkable antioxidant activities. Furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale, which may be attributed to its variable and high contents of flavonoids, bibenzyls and phenanthrenes. These results provide the evidence for the high quality and efficacy of D. huoshanense.

Probiotic fermentation improves the bioactivities and bioaccessibility of polyphenols in Dendrobium officinale under in vitro simulated gastrointestinal digestion and fecal fermentation

“…The contents of phenolic compounds of DO and FDO before and after in vitro digestion and colonic fermentation are tabulated in Table 2 . According to the previous studies, 16 standards were selected ( 37 – 39 ). These standards and compounds were identified and quantified using HPLC-DAD by comparing the retention times and peak area with their authentic standards.…”

Selection of Suitable Reference Genes for Gene Expression Normalization Studies in Dendrobium huoshanense

“…D. huoshanense has attracted many researchers because of its broad pharmacological activity [ 9 ]. Our research group on D. huoshanense has carried out a series of studies on the field resource protection, tissue culture and cultivation, chemical component separation and identification, bioactivity analysis and action mechanism, and new drug and product development [ 3 , 5 , 10 , 39 , 50 , 51 , 52 , 53 ], and also completed its whole genome sequencing [ 54 ]. In addition, the transcriptome sequencing of D. huoshanense had been reported several times in other research groups [ 13 , 14 , 15 , 16 , 17 ].…”

Identification of lignans and quality assessment in Dendrobium officinale under different cultivation modes

“…HPLC coupled with MS n using ESI ion trap mass spectrometry can obtain a large amount of fragmentation ion information and relationships between ions of different mass numbers can be identified, 17 which can determine the molecular weight and identify substances even if chromatography fails to completely separate them. This technology solves the problem of lack of reference materials and difficult separation of chemical components of most compounds and has unique advantages for complex extracts of traditional Chinese medicine, which is also of great significance in the study of chemical constituents of D. officinale 18,19 . In addition, natural lignan products with different structural characteristics can display fragment ions with different cleavage characteristics in the mass spectrum, which is the basis for structural identification of related compounds.…”