Comparative Analysis of Cell Ultrastructure with Evidences of Programmed Death in Tissue Cultures of Cereals

Abstract

Degrading cells of wheat and barley callus have all known ultrastructural features, which are characteristic for apoptotic cells: intense vacuolization, increasing the size of the nucleus, cupped invaginations of plastids, mitochondria saving until the last stages of cell degradation and the formation of vesicles, similar to apoptotic bodies. It is the first showed that degrading cells in embryogenic callus are characterized with the destruction of the nuclear membrane and the allocated substances of fibrillar nature in the periplasmic space and on the surface of cells.