Comparative Analysis of Bioactivity of the Russian-Made Antitumor Substances of the Nitrosourea Group

Toxicological Properties of 7-Methylguanine, and Preliminary Data on its Anticancer Activity

“…Six-week-old female CBA mice were obtained from the Stolbovaya farm. Uterine sarcoma US-322 and cervical squamous cell carcinoma RShM-5 (originally derived from CBA mice exposed to 1,2-dimethylhydrazine and 3-methylcholanthrene, respectively) ( Treshalina et al, 2000 ; Turusov et al, 2005 ; Bunyatyan et al, 2019 ) were inoculated subcutaneously by injecting 0.5 ml of tumor cell suspension (0.1 g/ml in PBS) into the right axillary cavity. Mice with US-322 were divided into three treatment groups of 10 animals: control group I, PBS (s.c., 3 times per week); group II, 7-MG (50 mg/kg per os, 3 times per week); group III, cisplatin (2.5 mg/kg s.c., 2 times within a week after inoculation).…”

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“…Six-week-old female CBA mice were obtained from the Stolbovaya farm. Uterine sarcoma US-322 and cervical squamous cell carcinoma RShM-5 (originally derived from CBA mice exposed to 1,2-dimethylhydrazine and 3methylcholanthrene, respectively) (Treshalina et al, 2000;Turusov et al, 2005;Bunyatyan et al, 2019) were inoculated subcutaneously by injecting 0.5 ml of tumor cell suspension (0.1 g/ml in PBS) into the right axillary cavity. Mice with US-322 were divided into three treatment groups of 10 animals: control group I, PBS (s.c., 3 times per week); group II, 7-MG (50 mg/kg per os, 3 times per week); group III, cisplatin (2.5 mg/kg s.c., 2 times within a week after inoculation).…”