Comparative analysis of basal locomotor activity-related metabolic phenotypes between C57BL/6 mice and ICR mice substrains derived from three different sources

Abstract

Animal model, as an indispensable tool for scientific purposes of biomedical researches and clinical application, is a commonly used in various researches. Regarding to this, it is necessary to establish the metabolic phenotype of animal model to minimize spurious interpretations and ensure a level of accuracy and reliability adequate for experimental research. However, the metabolic phenotype-related analysis within rodent strains derived from different source is nonexistent, especially in C57BL/6Korl mice and Korl:ICR mice (a domestic mouse strain). To compare the physiological and metabolic phenotypes over a period of time, we utilized the C57BL/6 mice (C57BL/6Korl, A:C57BL/6, and B:C57BL/6) and ICR mice (Korl:ICR, A:ICR, and B:ICR) derived from three different sources. Our data showed that average body weight, body temperature, food intake, and water consumption have a similar tendency among the C57BL/6 and ICR groups, except for the higher body weight of C57BL/6Korl mice over a period of time. Moreover, some significant differences was observed in adipose tissue mass and adipocyte size among the groups, with a higher tendency of C57BL/6Korl mice and Korl:ICR mice. Most importantly, resting metabolic rate (RMR) serves as an approximation of the metabolic phenotype showed no significant difference among the groups of C57BL/6 mice and ICR mice, except for the lower oxygen uptake of C57BL/6Korl mice compare to the A:C57BL/6 mice. Taken together, our data suggest that C57BL/6 mice and ICR mice derived from three different sources have an overall similar feature of physiological and metabolic phenotypes.

Inflammatory responses of C57BL/6NKorl mice to dextran sulfate sodium-induced colitis: comparison between three C57BL/6 N sub-strains

“…Prior to DSS treatment, C57BL/6NKorl mice had higher body weights compared to the other two sub-strains of C57BL/6 N mice (Fig. 1 a), in agreement with previous studies [ 9 , 10 , 12 , 14 ]. DSS caused dose- and time-dependent body weight loss (Fig.…”

“…The C57BL/6NKorl mice have shown similar humoral immunity, fertilization and embryo development rates, tumorigenesis, and responses to cisplatin and restraint stress compared to other C57BL/6 N mice [ 9 – 14 ]. However, relatively higher body weight and adipose tissue mass, and lower oxygen consumption were found to be distinct features of the C57BL/6NKorl sub-strain [ 9 , 10 , 12 , 14 ]. Moreover, cell-mediated immune response of C57BL/6NKorl mice determined by concanavalin A-induced splenic T cell proliferation was stronger than those of other C57BL/6 N sub-strains [ 11 ].…”

Taurine Stimulates Thermoregulatory Genes in Brown Fat Tissue and Muscle without an Influence on Inguinal White Fat Tissue in a High-Fat Diet-Induced Obese Mouse Model

“…Also, the C57BL/6 mice used as an obesity model is known to develop severe obesity when fed a high fat diet (HFD) due to their genetic susceptibility to diet-induced obesity (DIO) [ 21 ]. Even though C57BL/6 and ICR mice overall have similar physiological and metabolic phenotypes [ 22 ], C57BL/6 HFD fed mice also develop dyslipidemia and changes in glucose homeostasis resembling a pre-diabetic condition, whereas ICR mice show mild obesity without any pre-diabetic conditions. Therefore, ICR HFD fed mice may be a more suitable model system for mimicking mild obesity similar to humans than C57BL/6 mice.…”

Physicochemical and Biochemical Evaluation of Amorphous Solid Dispersion of Naringenin Prepared Using Hot-Melt Extrusion

“…Food intake increased significantly by day 9 for females administered SD and decreased by day 14 for males administered SD. Nevertheless, these changes were still within the normal range ( 28 ). The weights of the livers of the female mice administered NRG SD significantly increased relative to those of the control.…”