Comparative analysis of BABA and Piriformospora indica mediated priming of defence-related genes in tomato against early blight

Roylawar, Praveen; Panda, Sayantan; Kamble, Avinash

doi:10.1016/j.pmpp.2015.06.004

Cited by 34 publications

(22 citation statements)

References 40 publications

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“…Since the elicitation of PR gene expression and phytoalexin showed no or limited increase, the action mode of BABA likely occurred in parallel with the priming of defenses. This priming resistance that activated the plant defense responses under high pathogenic stress was also observed for chitosan, MeJA, EBR, BTH, and BABA in Arabidopsis, peaches, bayberries, and tomatoes (Jia et al, 2020;Kohler & Conrath, 2002;Li, Du, & Wang, 2020;Roylawar et al, 2015;Wang et al, 2014). Intriguingly, etr1-1 and coi1-1 mutants defective in the ethylene-and jasmonate (JA)-dependent pathways, respectively, were more sensitive than WT (Col-0 ecotype) to B. cinerea inoculation, but they exhibited similar levels of fungal growth to WT Arabidopsis (Cerrudo et al, 2012;Kishimoto et al, 2006), suggesting an independence of BABA-mediated resistance against the fungus B. cinerea from ethylene and JA signaling pathways.…”

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confidence: 58%

Dual function of VvWRKY18 transcription factor in the β‐aminobutyric acid‐activated priming defense in grapes

Wang

Lei

et al. 2021

Physiologia Plantarum

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Induction of phytoalexin production after invading pathogens is recognized as an essential aspect of the plant-induced resistance. The WRKY family includes plantspecific transcriptional factors associated with plant defense responses, but the comprehensive mechanisms are poorly understood. Here, we attempted to elaborate the regulatory function of VvWRKY18 from the group IIa of WRKY transcription factor (TF) from Vitis vinifera, in the regulation of β-aminobutyric acid (BABA)-activated stilbene phytoalexins biosynthesis and PATHOGENESIS-RELATED (PR) genes expressions in grapes. BABA at 10 mmol L −1 triggered a priming protection in grapes and conferred a potentiation of the expression levels of VvWRKY18, VvNPR1, and several salicylic acid (SA)-responsive genes, which was accompanied by enhanced stilbene production upon Botrytis cinerea infection. In addition, a physical interaction between VvWRKY18 and the regulatory protein VvNPR1 was detected in vivo and in vitro by yeast-2-hybrid (Y2H), pull-down and co-immunoprecipitation assay (Co-IP) assays. Furthermore, yeast-1-hybrid (Y1H) and dual-luciferase reporter (DLR) assays indicated that VvWRKY18 activated the transcription of STILBENE SYNTHASE (STS) genes, including VvSTS1 and VvSTS2, by directly binding the W-box elements within the specific promoters and resultantly enhancing stilbene phytoalexins biosynthesis. Further investigation demonstrated that heterologous expression of VvWRKY18 elevated the transcriptions of STS and PR genes, thus contributing to potentiating the defense of transgenic Arabidopsis thaliana plants and resultantly inhibiting B. cinerea invasion. Hence, VvWRKY18 serves as a singular effector involved in the synthesis of stilbene phytoalexins in grapes and its interaction with VvNPR1 provided DNA binding ability required for VvNPR1 to initiate systemic acquired resistance (SAR) defense. | INTRODUCTIONGrapevine (Vitis vinifera L.) has considerable commercial importance worldwide in terms of its pleasant flavor and abundant phytonutrient composition (Dai et al., 2011). However, postharvest grape fruit has a limited shelf-life, which is mainly attributable to the invasion of pathogens and gray mold caused by Botrytis cinerea (Pers.: Fr), which is thought to be one of the most serious diseases that occurs during the postharvest period (Feliziani et al., 2013). This necrotrophic fungus mainly secretes various cell wall-degrading enzymes and mycotoxins to infect grapevines or berries (Gabler et al., 2003). The persistent use of chemical fungicides on grapevines to control gray mold rot is effective, but the development of resistant strains has also occurred. In addition, the frequent use of fungicides has given rise to considerable negative impacts on environmental and public health; thus, ecocompatible options for replacing the harmful fungicides for suppression of gray mold rot in grape berries are urgently needed (Katan, 2017).

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confidence: 58%

Dual function of VvWRKY18 transcription factor in the β‐aminobutyric acid‐activated priming defense in grapes

Wang

Lei

et al. 2021

Physiologia Plantarum

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“…Elevated AtWRKY33 expression was reported in response to B. cineria (Zheng et al ., ). During BABA‐induced resistance, necrotrophic inoculation resulted in higher levels of BjWRKY11 and SlWRKY11 in brassica and tomato, respectively (Chavan and Kamble, ; Roylawar et al ., ). Consistence with above reports, SaWRKY11 and SaWRKY40 levels were increased in R accession.…”

Section: Discussionmentioning

confidence: 97%

WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta

Shinde

Dholakia

Hussain

et al. 2018

Plant Biotechnology Journal

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SummaryEarly blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio‐temporal molecular events in S. arcanum against A. solani. Transcriptome and co‐expression analysis presented 33‐WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro‐mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence‐related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A. solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A. solani in crop plants.

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“…Trichoderma has also been shown to induce systemic resistance against nematode, which adapts its priming of salicyclic acid (SA)-and jasmonic acid (JA)-related defense responses according to the stage of the nematode infection cycle [236]. Roylawar et al [237] found that the pre-colonization of roots with P. indica resulted in decreased disease severity in tomato caused by early blight. They concluded that P. indica treatments led to priming of defense-related gene expression in tomato.…”

Section: Root Endophytes and Tree Healthmentioning

confidence: 99%

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

164

113

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Terrestrial plants including forest trees are generally known to live in close association with microbial organisms. The inherent features of this close association can be commensalism, parasitism or mutualism. The term “microbiota” has been used to describe this ecological community of plant-associated pathogenic, mutualistic, endophytic and commensal microorganisms. Many of these microbiota inhabiting forest trees could have a potential impact on the health of, and disease progression in, forest biomes. Comparatively, studies on forest tree microbiomes and their roles in mutualism and disease lag far behind parallel work on crop and human microbiome projects. Very recently, our understanding of plant and tree microbiomes has been enriched due to novel technological advances using metabarcoding, metagenomics, metatranscriptomics and metaproteomics approaches. In addition, the availability of massive DNA databases (e.g., NCBI (USA), EMBL (Europe), DDBJ (Japan), UNITE (Estonia)) as well as powerful computational and bioinformatics tools has helped to facilitate data mining by researchers across diverse disciplines. Available data demonstrate that plant phyllosphere bacterial communities are dominated by members of only a few phyla (Proteobacteria, Actinobacteria, Bacteroidetes). In bulk forest soil, the dominant fungal group is Basidiomycota, whereas Ascomycota is the most prevalent group within plant tissues. The current challenge, however, is how to harness and link the acquired knowledge on microbiomes for translational forest management. Among tree-associated microorganisms, endophytic fungal biota are attracting a lot of attention for their beneficial health- and growth-promoting effects, and were preferentially discussed in this review.

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Comparative analysis of BABA and Piriformospora indica mediated priming of defence-related genes in tomato against early blight

Cited by 34 publications

References 40 publications

Dual function of VvWRKY18 transcription factor in the β‐aminobutyric acid‐activated priming defense in grapes

Dual function of VvWRKY18 transcription factor in the β‐aminobutyric acid‐activated priming defense in grapes

WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

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