Comparative analysis of ascorbate peroxidases (APXs) from selected plants with a special focus on Oryza sativa employing public databases

Abstract

Reactive oxygen species (ROS) are produced by plants. Hydrogen peroxide (H2O2) is one important component of ROS and able to modulate plant growth and development at low level and damage plant cells at high concentrations. Ascorbate peroxidase (APX) shows high affinity towards H2O2 and plays vital roles in H2O2-scavenging. In order to explore the differences of APXs from selected plant species, bioinformatics methods and public databases were used to evaluate the physicochemical properties, conserved motifs, potential modifications and cis-elements in all the APXs, and protein-protein network and expression profiles of rice APXs. The results suggested that APXs in the selected plant species showed high evolutionary conservation and were able to divide into seven groups, group I to VII. Members in the groups contained abundant phosphorylation sites. Interestingly, group I and VII had only PKC site. Additionally, promoters of the APXs contained abundant stress-related cis-elements. APXs in rice plant were able to interact with dehydroascorbate reductase 2. The eight APXs expressed differently in root, leaf, panicle, anther, pistil and seed. Drought, Pi-free, Cd and Xanthomonas oryzae pv. oryzicola B8-12 treatments were able to significantly alter the expression profiles of rice APXs. This study increases our knowledge to further explore functions and mechanisms of APXs and also guides their applications.

Proteome Landscape during Ripening of Solid Endosperm from Two Different Coconut Cultivars Reveals Contrasting Carbohydrate and Fatty Acid Metabolic Pathway Modulation

“…Regarding APX2, it detoxifies the H 2 O 2 during biotic and abiotic stresses [ 49 ]. APX2 was down-accumulated in the mature stage, while glutathione S-transferase (GST) was up-accumulated ( Figure 3 H), showing that antioxidant proteins are well coordinated in the MPT endosperm ( Figure 4 ) to cope with different kinds of stresses.…”

Identification and Characterization of the APX Gene Family and Its Expression Pattern under Phytohormone Treatment and Abiotic Stress in Populus trichocarpa

“…Ascorbate peroxidase genes play an important role in the detoxification of photoproduced H 2 O 2 and diverse abiotic stresses, such as drought, low temperature, high salinity, and iron stress [48]. APX genes have been characterized in, for example, Arabidopsis [8,49], rice [11,50], tomato (Solanum lycopersicum) [51], and cotton (Gossypium hirsutum) [52]. Limited information on the APX gene family is available for the model woody plant, Populus trichocarpa.…”

Identification of differentially expressed transcripts for trunk formation in sago palm using annealing control primer GeneFishing technique

“…The transcript for cytosolic APX was found with a higher abundance in T samples (81%). In other studies, a few types of APXs accumulate in stress-induced plants [20,21]. APX activity was also reported to upregulate in response to heavy metal, drought, water, and heat stress [22,23].…”