Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts

Abstract

Medicinal plants are used to control and remediate oxidative stress related diseases caused by free radicals. Thus, these plants find their use as remedy. Moringa oleifera is an extremely valued plant for its medicinal properties. Herein, two indigenously produced accessions of Moringa oleifera seeds [originated from Multan (M-Mln) and India (PKM1)] were investigated for their antioxidant properties by 2.2-Diphenyl-1picrylhydrazyl (DPPH) assay, total phenolics content and total flavonoids content. The presence of various phenolics as well as flavonoids was further confirmed by high performance liquid chromatography. Moreover, fourier transform infrared spectroscopy detected the presence of various functional groups. In conclusion, these findings revealed that the methanol extract of M-Mln variety seeds showed high antioxidant potential, having IC50 value of 84 μg/ml. While, hexane extract of PKM1 showed least activity. The methanol extract of M-Mln was found to show highest total phenolics content as 33.83 ± 1.19 mg GAE/g. The methanol extract of M-Mln was found to show highest total flavonoids content as 76.07 ± 1.10 mg CAE/g. The hexane extract of PKM1 was found to show least total flavonoids content as 22.47 ± 1.70 mg CAE/g. The detection of phenolics (ferulic acid, caffeic acid, chlorogenic acid, coumaric acid, and gallic acid) as well as flavonoids (catechin and quercetin) revealed the potential of methanol extracts of both varieties as a good source of antioxidants. The results indicated the importance of seed extracts in the treatment of oxidative stress related diseases. In future, the use of natural antioxidants will prevent the progression of diseases.

In vitro antioxidant and free-radical scavenging activities of polar leaf extracts of Vernonia amygdalina

“…While the standard antioxidant had an IC50 of 127.737 μg/ml. Low IC 50 values correspond to high antioxidant activity [ 52 ]. Thus, the EtOH extract had the highest antioxidant activity among the other leaves extract which has the smallest IC50 value at higher concentration.…”

Evaluation of antioxidant and antibacterial properties of dehydrocostus lactone isolated from Echinops kebericho root

“…40 The antioxidant activity of the three solvents extracts, DHCL and ascorbic acid against DPPH assay was tested with concentrations ranging from 50 to 300 μg/mL as the results shown in Table 4. As shown in 41 Thus, pure isolated DHCL had the highest antioxidant activity compared with crude extracts and EtOH extract showed higher inhibitory activity among the other root extracts. From these results, it was also possible to make several correlations regarding the relationship between the structure of isolated compound DHCL and its DPPH-scavenging activities.…”

“…The IC50 values of DPPH assay for the hexane, EtOAc, MeOH, and DHCL were 109.70, 95.34, 94.71, and 93.10, respectively, as compared with standard AA 91.82. Low IC50 values correspond to high antioxidant activity 41 …”

Retracted: Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Manipulated or compromised peer review …”

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”