Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods

Abstract

Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This “swab in—profile out” method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equipment (ANDE) 6C system as a typing method for reference samples according to the ISO/IEC 17025 standard. Here, we report the evaluation of the validity and reproducibility of results by the comparison of the genetic profiles generated by the ANDE 6C System with those generated by standard technologies. A quantity of 104 buccal swabs were analyzed both through the ANDE 6C technology and the traditional method (DNA extraction and quantification, amplification and separation by capillary electrophoresis). Positive typing was observed in 97% of cases for ANDE 6C technology with only three buccal swabs failing to reveal interpretable signals. Concordance was determined by comparing the allele calls generated by ANDE 6C and conventional technology. Comparison of 2800 genotypes revealed a concordance rate of 99.96%. These results met the ISO/IEC 17025 requirements, enabling us to receive the accreditation for this method. Finally, rapid technology has certainly reached a level of reliability which has made its use in laboratories of forensic genetics a reality.

Evaluation of OpenArray™ as a Genotyping Method for Forensic DNA Phenotyping and Human Identification

“…The OpenArray™ System (Thermo Fisher Scientific, Waltham, MA, USA) successfully analyzed 100% of the reference DNA samples. The average call rate and the average concordance rate were 96.9 ± 1.72 and 99.8 ± 0.56, respectively [ 25 ]. The call rate was also calculated for each SNP.…”

“…The amplification was performed using the QuantStudio 12K Flex Real Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) instrument, and the results were analyzed using TaqMan Genotyper Software (Thermo Fisher Scientific, Waltham, MA, USA) [ 14 ]. For each SNP, the call rate and concordance rate were calculated as described in Section 2.7 [ 25 ].…”

“…The concordance rate is defined as the proportion of SNPs typed by OpenArray™ confirming the resequencing data. This value was determined by comparing the allele calls generated by OpenArray™ technology with the genotypes resulting from resequencing data [ 25 ]. Concordance is the percentage of SNPs with identical results using both OpenArray™ and resequencing.…”

Special Issue “Forensic Genetics and Genomics”

“…Continuing the topic of on-site genetic analysis, the work of Ragazzo M et al Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods compares the rapid analysis of DNA using the ANDE 6C with that of capillary electrophoresis [ 6 ]. The results met the ISO/IEC 17025 requirements, enabling ANDE 6C to receive accreditation for the rapid method.…”

“…The results met the ISO/IEC 17025 requirements, enabling ANDE 6C to receive accreditation for the rapid method. This means that rapid technology has certainly reached a level of reliability which has made its use in forensic genetic laboratories a reality [ 6 ].…”