Comparative analysis of an IncR plasmid carryingarmA,blaDHA-1andqnrB4fromKlebsiella pneumoniaeST37 isolates

Guo, Qinglan; Spychala, Caressa N.; McElheny, Christi L.; Doi, Yohei

doi:10.1093/jac/dkv444

Cited by 35 publications

(20 citation statements)

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“…And our data indicated that ST37 were the main epidemic clones in the Neonate Ward, which showed consistency with what found in other studies [19,38]. It was discovered that ST37 are presumably to be a potential high-risk MDR K.pneumoniae clonal lineage [39].Furthermore, it is reported that carriage of carbapenem-resistant K. pneumoniae (CRKP) in the GI tract may precede and possibly serve as a source for subsequent clinical infections in approximately 9% of carriers [40,41]. And these carriers may act as a signi cant reservoir for the dissemination of CRKP in the healthcare facilities [42~44].Combined with our study, active surveillance for detecting CRKP colonization is critical for preventing the CRKP from spreading.…”

Section: Discussionsupporting

confidence: 92%

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

Chen

Wang²,

et al. 2020

Preprint

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Background: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported in many studies that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. But the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn’t been characterized yet.Results: 43 isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. Conclusions: These data showed that there might be a close homologous relationship between EXKP and EKP in neonates，indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

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Section: Discussionsupporting

confidence: 92%

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

Chen

Wang²,

et al. 2020

Preprint

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“…Plasmids with the R repB gene alone are apparently nonconjugative, lacking tra genes, and no relaxase gene has been identified (267). This may explain why complete plasmid sequences with R repB often have an additional replicon including FII K , A/C, or untyped rep genes (267,268). The original report of pK245 (266) also noted that repB is closely related to the ␤ replicon of pGSH500 (isolated from K. pneumoniae in/prior to 1991), which also carries an FII-like (␣) replicon (269).…”

Section: Resistance Plasmids In the Enterobacteriaceaementioning

confidence: 99%

Mobile Genetic Elements Associated with Antimicrobial Resistance

et al. 2018

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SUMMARYStrains of bacteria resistant to antibiotics, particularly those that are multiresistant, are an increasing major health care problem around the world. It is now abundantly clear that both Gram-negative and Gram-positive bacteria are able to meet the evolutionary challenge of combating antimicrobial chemotherapy, often by acquiring preexisting resistance determinants from the bacterial gene pool. This is achieved through the concerted activities of mobile genetic elements able to move within or between DNA molecules, which include insertion sequences, transposons, and gene cassettes/integrons, and those that are able to transfer between bacterial cells, such as plasmids and integrative conjugative elements. Together these elements play a central role in facilitating horizontal genetic exchange and therefore promote the acquisition and spread of resistance genes. This review aims to outline the characteristics of the major types of mobile genetic elements involved in acquisition and spread of antibiotic resistance in both Gram-negative and Gram-positive bacteria, focusing on the so-called ESKAPEE group of organisms (, ,, ,, spp., and), which have become the most problematic hospital pathogens.

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“…Therefore, we should also pay close attention to the sporadic ST in addition to the major epidemic clone complex. It was discovered that ST37 are international clones associated with multidrug-resistant K. pneumoniae such as extended-spectrum β-lactamase, AmpC or carbapenemase producers [26]. Recently, NDM-1-producing K. pneumoniae belonging to ST37 were reported in China [27], which indicated that multidrug-resistant K. pneumoniae ST37 have been identified in our country.…”

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confidence: 95%

ST37 Klebsiella Pneumoniae : Development of Carbapenem Resistance in Vivo During Antimicrobial Therapy in Neonates

Wang

et al. 2017

Future Microbiol.

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Aim:To investigate the mechanism leading to in vivo carbapenem resistance development in Klebsiella pneumoniae. Methods: Carbapenemase was detected using the modified carbapenem inactivation method. β-lactamases resistant genes were identified by PCR and sequencing. Clonal relatedness was evaluated by random amplified polymorphic DNA and multiple locus sequence typing. The relationship between sequence typing and resistant genes was analyzed by using the chi-squared test. Results: All ST37 carbapenem-resistant isolates were bla OXA-1 positive and all ST37 carbapenem-sensitive isolates were bla OXA-1 negative at Stage I. A significant relationship between carbapenem resistance and bla OXA-1 was observed. The bla OXA-1 -positive rate was significantly higher in ST37 K. pneumoniae than others. Conclusion: This is the first study about the development of carbapenem resistance in vivo potentially mediated by bla OXA-1 in ST37 K. pneumoniae among neonates.

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Comparative analysis of an IncR plasmid carryingarmA,bla_DHA-1andqnrB4fromKlebsiella pneumoniaeST37 isolates

Abstract: K. pneumoniae ST37 strains with an MDR IncR plasmid carrying armA, blaDHA-1 and qnrB4 were identified in a hospital in the USA, where these resistance genes remain rare. The IncR backbone may play a role in the global dissemination of these resistance genes.

Cited by 35 publications

References 32 publications

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among Neonates

Mobile Genetic Elements Associated with Antimicrobial Resistance

ST37 Klebsiella Pneumoniae : Development of Carbapenem Resistance in Vivo During Antimicrobial Therapy in Neonates

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