Comparative analysis of alternative splicing, alternative polyadenylation and the expression of the two KIN genes from cytoplasmic male sterility cabbage (Brassica oleracea L. var. capitata L.)

Abstract

The KIN genes are crucial members of the cold-regulated gene family. They play exclusive roles during the developmental processes of many organs and respond to various abiotic stresses in plants. However, little is known about the regulation of KIN gene expression in cytoplasmic male sterility (CMS) cabbages (Brassica oleracea L. var. capitata L.). We carried out a genome-wide analysis to identify the KIN genes in the CMS cabbage. Two non-redundant KIN genes, named BoKIN1 (Bol021262) and BoKIN2 (Bol030498), were identified. Reverse transcriptase PCR detected alternative splicing (AS) products of BoKIN1 (four AS products) and BoKIN2 (three AS products). In addition, alternative polyadenylation (APA) was observed for BoKIN1 and BoKIN2 in the CMS cabbage, resulting in variable 3'UTRs in their transcripts. Furthermore, the transcription levels of BoKIN1-0 and BoKIN2-0, the introns of which were spliced completely, were analyzed in various organs and young leaves treated by abiotic stresses. Our data indicated that BoKIN1-0 is highly expressed in various organs, whereas BoKIN2-0 is expressed exclusively in the stamen. Our study also suggested that BoKIN1-0 was upregulated significantly in young leaves of plants exposed to abscisic acid treatment, and cold and heat stress. BoKIN1 and BoKIN2 had differential AS and APA patterns in pre-mRNA processing, and showed differences in their expression patterns and transcript levels. BoKIN1 participates widely in organ development and responds to diverse abiotic stresses, whereas BoKIN2 plays a main role in stamen development in the CMS cabbage.

Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato

“…2 a). This is consistent with what has been observed for other Kin family members such as BN28 of Brassica napus 32 and BoKIN1 of B. oleracea 33 and is also consistent with its proposed role in the process of cold acclimation, wherein the leaf seems to bear the major brunt of cold stress compared to root and stem. These results are therefore suggestive that BoCRP1 have role in imparting cold tolerance that was further validated through its functional characterization in cold susceptible tomato.…”

Ectopic Expression of a Novel Cold-resistance Protein 1 (BoCRP1) from Brassica Oleracea Promotes Tolerance to Cold via Modulating Stress Associated Components

“…2a). This is consistent with what has been observed for other Kin family members such as BN28 of Brassica napus 31 and BoKIN1 of B. oleracea 32 and is also consistent with its proposed role in the process of cold acclimation, wherein the leaf seems to bear the major brunt of cold stress compared to root and stem. These results are therefore suggestive of BoCRP1 role in imparting cold tolerance that was further validated through its functional characterization in cold susceptible tomato.…”

Genome-wide identification and characterization of aquaporin genes (AQPs) in Chinese cabbage (Brassica rapa ssp. pekinensis)

“…Paralogs are genes in the same genome created by gene duplication events that may have different functions (Thornton and DeSalle 2000). Tao et al (2014) observed that two paralogous genes from B. oleracea L. var. capitata L. (BoKIN1 and BoKIN2) showed discrepancies not only in their expression patterns and transcript levels, but also in premRNA processing.…”