Comparative analysis of abscisic acid levels and expression of abscisic acid-related genes in Scots pine and Norway spruce seedlings under water deficit

Pashkovskiy, Pavel P.; Vaňková, Radomı́ra; Zlobin, Ilya E.; Dobrev, Petre I.; Ivanov, Yu. V.; Кузнецов, В. В.

doi:10.1016/j.plaphy.2019.04.037

Cited by 41 publications

(25 citation statements)

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“…In needles, metabolism remained largely unchanged, with no enrichment for terms associated with the primary physiological mechanisms of photosynthesis. Pashkovskiy et al. (2019 ) reported ABA levels in Norway spruce and Scots pine roots and needles in response to drought, finding significantly higher baseline ABA levels and more substantial induction in Norway spruce needles than roots.…”

Section: Discussionmentioning

confidence: 99%

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Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

et al. 2021

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Drought stress impacts seedling establishment, survival and whole-plant productivity. Molecular responses to drought stress have been most extensively studied in herbaceous species, mostly considering only aboveground tissues. Coniferous tree species dominate boreal forests, which are predicted to be exposed to more frequent and acute drought as a result of ongoing climate change. The associated impact at all stages of the forest tree life cycle is expected to have large-scale ecological and economic impacts. However, the molecular response to drought has not been comprehensively profiled for coniferous species. We assayed the physiological and transcriptional response of Picea abies (L.) H. Karst seedling needles and roots after exposure to mild and severe drought. Shoots and needles showed extensive reversible plasticity for physiological measures indicative of drought response mechanisms, including changes in stomatal conductance (gs), shoot water potential and ABA (abscisic acid). In both tissues the most commonly observed expression profiles in response to drought were highly correlated with ABA levels. Still, root and needle transcriptional responses contrasted, with extensive root-specific downregulation of growth. Comparison between previously characterized A. thaliana drought-response genes and P. abies revealed both conservation and divergence of transcriptional response to drought. In P. abies, transcription factors belonging to the bZIP AREB/ABF (ABA Response Element Binding/ABRE Binding Factors) ABA-dependent pathway had a more limited role. These results highlight the importance of profiling both above- and below-ground tissues and provide a comprehensive framework to advance understanding of the drought response of P. abies. The results demonstrate that short term, severe drought induces severe physiological responses coupled to extensive transcriptome modulation and highlight the susceptibility of Norway spruce seedlings to such drought events.

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Section: Discussionmentioning

confidence: 99%

“…These have established that ABA has a conserved role in controlling drought-response mechanisms ( Brodribb and McAdam 2013 , Brodribb et al. 2014 , Pashkovskiy et al. 2019 ) and that there is genetically controlled variation in drought response within and between species ( Moran et al.…”

Section: Introductionmentioning

confidence: 99%

Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

et al. 2021

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“…More isohydric species use permanently high ABA levels to close stomata and maintain leaf water homeostasis during drought, while more anisohydric species rely on leaf desiccation to passively drive stomatal closure. Picea abies is a rather isohydric species [13,38], with a conservative-strategy in drought responses.…”

Section: Discussionmentioning

confidence: 99%

“…However, whereas the physiological response of spruce to drought stress has been researched [11][12][13], only a few studies addressed biochemical responses in the functional traits of spruce provenances. After the exposure of plants to drought and heat stress, a number of biochemical and physiological changes are induced.…”

Section: Introductionmentioning

confidence: 99%

Norway Spruce (Picea abies L.) Provenances Use Different Physiological Strategies to Cope with Water Deficit

Jamnická

Fleischer

Konôpková

et al. 2019

Forests

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Research Highlights: In this study, we found different regulatory mechanisms in two contrasting provenances of Norway spruce responding to progressive drought stress. Background and Objectives: In the context of climate and environmental changes, the following question of high importance in scientific studies is: How will Norway spruce, which forms a dominant component in European mountain and boreal forests, be able to cope with the increasing frequencies and intensities of drought periods? The aim of the study was to investigate the physiological responses of eight-month-old seedlings, as a most vulnerable phase of forest tree life, for different spruce provenances, to find out variable strategies in relation to controlled drought stress. Materials and Methods: We performed an experiment under controlled conditions with spruce seedlings from a climatologically warmer stand of 410 m above sea level (a.s.l.), and a moderately cool stand of 931 m a.s.l. The soil water content, leaf predawn water potential, the osmotic potential of needles, the relative water content of needles, and the photosynthetic performance and the contents of primary metabolites (proline and abscisic acid) were investigated as indicators of the spruce seedlings’ responses to water stress. The status of the indicators was analyzed at different temporal intervals, using repeated measures analysis of variance (ANOVA), reflecting the seedlings´ water conditions (early drought, drought, or recovery). On a daily basis, the differences among the indicators were tested with one-way ANOVA. A principal component analysis (PCA) was used to identify the provenance-specific responses of tree drought indicators in a multi-dimensional data set. Results: The responses to drought stress differed between the provenances. Whereas seedlings of ´warm´ provenance from a low altitude performed a conservation strategy, with high amounts of accumulated abscisic acid and closing the stomata faster, the reactions of ´cool´ provenance seedlings from a higher altitude were not so sensitive and the plants’ water supply and photosynthetic performance remained significantly higher. These findings indicate that a higher drought resistance in ´cool´ provenance could be related to greater amounts of proline amino-acids, which are accumulated from the beginning of the drought simulation. Furthermore, proline accumulation resulting in increased stress tolerance is controlled through another mechanism than osmotic adjustment. Conclusions: The observed variations in the regulatory mechanisms used to develop adaptive strategies in different provenances are an important factor for seedling survival under a changing climate.

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“…In summary, these results indicate that HXK1 plays a vital role in the ABA‐induced NO and H 2 O 2 production that is involved in stomatal closure and drought tolerance in herbaceous plants (Fancy et al ., 2017; Kelly et al ., 2019). The main ABA signaling components and pathways that mediate stomatal closure are conserved between herbaceous and woody plants (Papacek et al ., 2017; Pashkovskiy et al ., 2019; Estravis‐Barcala et al ., 2020). Under drought stress, the poplar protein CHYR1, an ubiquitin E3 ligase, promotes stomatal closure via reactive oxygen species signaling (He et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H₂O₂ production via the direct regulation of PdHXK1 expression in Populus

Shen

Zhang

et al. 2021

New Phytologist

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Drought is one of the primary abiotic stresses, seriously implicating plant growth and productivity. Stomata play a crucial role in regulating drought tolerance. However, the molecular mechanism on stomatal movement-mediated drought tolerance remains unclear.Using genetic, molecular and biochemical techniques, we identified that the PdGNC directly activating the promoter of PdHXK1 by binding the GATC element, a hexokinase (HXK) synthesis key gene.Here, PdGNC, a member of the GATA transcription factor family, was greatly induced by abscisic acid and dehydration. Overexpressing PdGNC in poplar (Populus clone 717) resulted in reduced stomatal aperture with greater water-use efficiency and increased water deficit tolerance. By contrast, CRISPR/Cas9-mediated poplar mutant gnc exhibited increased stomatal aperture and water loss with reducing drought resistance. PdGNC activates PdHXK1 (a hexokinase synthesis key gene), resulting in a remarkable increase in hexokinase activity in poplars subjected to water deficit. Furthermore, hexokinase promoted nitric oxide (NO) and hydrogen peroxide (H 2 O 2 ) production in guard cells, which ultimately reduced stomatal aperture and increased drought resistance.Together, PdGNC confers drought stress tolerance by reducing stomatal aperture caused by NO and H 2 O 2 production via the direct regulation of PdHXK1 expression in poplars.

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Comparative analysis of abscisic acid levels and expression of abscisic acid-related genes in Scots pine and Norway spruce seedlings under water deficit

Cited by 41 publications

References 50 publications

Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

Norway Spruce (Picea abies L.) Provenances Use Different Physiological Strategies to Cope with Water Deficit

PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H₂O₂ production via the direct regulation of PdHXK1 expression in Populus

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Comparative analysis of abscisic acid levels and expression of abscisic acid-related genes in Scots pine and Norway spruce seedlings under water deficit

Cited by 41 publications

References 50 publications

Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

Candidate regulators and target genes of drought stress in needles and roots of Norway spruce

Norway Spruce (Picea abies L.) Provenances Use Different Physiological Strategies to Cope with Water Deficit

PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H2O2 production via the direct regulation of PdHXK1 expression in Populus

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PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H₂O₂ production via the direct regulation of PdHXK1 expression in Populus