Comparative analysis of a complement fixation assay and enzyme immunoassay to determine the seroprevalence of measles and varicella in a survey of healthcare workers

Abstract

Differences between the CF assay and EIA in detecting seronegative or seropositive healthcare workers for measles and varicella suggest that undertaking a seroprevalence survey using an EIA, rather than a CF assay, would more accurately determine susceptibility to vaccine-preventable diseases, in healthcare settings.

Vaccines for Health Care Personnel

“…39,40 Among commercially available test methodologies to detect IgG, enzyme immunoassay (EIA) is the most sensitive. 41,42 Although IgG provides reasonable, if imperfect, evidence of immunity, postvaccination serologic surveillance is not recommended.…”