Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe

Dzięgiel, Beata; Adaszek, Łukasz; Winiarczyk, Mateusz; García‐Bocanegra, Ignacio; Carbonero, Alfonso; Dębiak, Piotr; Winiarczyk, S.

doi:10.1099/jmm.0.058636-0

Cited by 11 publications

(10 citation statements)

References 25 publications

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“…Infections with A. phagocytophilum , the causative agent of human granulocytic anaplasmosis, have been increasingly diagnosed in both companion and farm animals in Poland (Adaszek et al 2009 ; Zygner et al 2009 ; Dzięgiel et al 2013 ). This pathogen has also been found in the blood of game animals (Adaszek et al 2012 ; Dzięgiel et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR

Dzięgiel

Adaszek

Carbonero³

et al. 2015

Parasitol Res

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The aim of the study was to establish the prevalence of Ehrlichia canis, Anaplasma phagocytophilum and Borrelia burgdorferi in dogs in eastern Poland and to determine the factors associated with exposure (seroposity) or infection (PCR). Anti-A. phagocytophilum, anti-B. burgdorferi and anti-E. canis antibodies were determined in 400 dogs, using the SNAP 4Dx ® test (IDEXX Laboratories). In addition, PCRs were performed for the detection of E. canis, A. phagocytophilum and B. burgdorferi DNA. In reference to the risk factor analysis, a regression logistic model was determined for each aetiological agent. The overall seroprevalence was highest for B. burgdorferi (11.0 %), followed by A. phagocytophilum (8.0 %) and E. canis (1.5 %). Eleven healthy dogs were found to be infected with A. phagocytophilum, as determined by PCR, while the remainder were seronegative. For B. burgdorferi, the DNA of the spirochetes was detected in the blood of 20 dogs, while the presence of anti-B. burgdorferi IgG was detected in the sera of ten of these. For E. canis, none of the dogs tested positive by PCR. Tick control was included as a protective factor for A. phagocytophilum and B. burgdorferi, while the origin (rural) was included as a risk factor for B. burgdorferi and A. phagocytophilum infection. In addition, breed (pure) was a risk factor for B. burgdorferi infection, and sex (female) was a risk factor for E. canis.

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Section: Introductionmentioning

confidence: 99%

Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR

Dzięgiel

Adaszek

Carbonero³

et al. 2015

Parasitol Res

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“…Therefore, we cannot exclude that horses in this study had previously been in contact with this pathogen. It is discussed that climate change might increase the threat of disease in European horse populations (41).…”

Section: Discussionmentioning

confidence: 99%

Survey of Zoonotic and Non-zoonotic Vector-Borne Pathogens in Military Horses in Lisbon, Portugal

Fuehrer

Alho²,

Kayikci

et al. 2020

Front. Vet. Sci.

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Vector-borne diseases of zoonotic and/or veterinary relevance have been increasingly reported in horses globally, although data regarding working and military horses is lacking. Portuguese military horses may constitute a risk group for these pathogens, as they frequently work outdoors in various regions of the country. This study included 101 apparently healthy horses belonging to the Portuguese National Republican Guard. Blood samples were analyzed to determine the presence and prevalence of piroplasms, Anaplasmataceae, Rickettsia spp., and filarioid helminths. Overall 32.7% of the horses gave positive results for Theileria equi. Two genotypes of T. equi were verified. No positive results were recorded for Anaplasma spp., Rickettsia spp., filarioid helminthes, and Babesia caballi. As equine piroplasmosis is a severe infectious tick-borne disease responsible for significant losses in equine production and with numerous impacts in the international movement of horses, adequate treatment, and preventive measures are needed to reduce exposure to vectors and future infections.

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“…10 Recently, a 16S rRNA nucleotide sequences investigation reported a new rickettsial genotype in horses in southern and eastern Europe. 11 Moreover, this gene (EC9/EC12a and SSAP2f/SSAP2r primer set) is quite accurate for the diagnosis of A. phagocytophilum DNA in ruminants and ticks. 12 Some studies conducted in Turkey have been reported DNA positivity for A. phagocytophilum in dogs, cattle, sheep and tick.…”

Section: Introductionmentioning

confidence: 98%

Türkiye’nin Muş İlinde Atlarda Anaplasma phagocytophilum’un İlk Moleküler Tespiti ve Filogenetik Analizi

Oğuz

2021

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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Objective: Equine granulocytic anaplasmosis (EGA) caused by Anaplasma phagocytophilum is a disease that generally develop characteristic high fever and a number of changes in blood such as thrombocytopenia. The main transmission of A. phagocytophilum is hard ticks through intrastadially. The aim of this study was to determine the presence of A. phagocytophilum in horses in Muş province of Turkey by using molecular methods. Methods: In the present survey, blood samples were collected in Muş province from 93 horse. Commercial ELISA kit (IgG) and nested-PCR were performed. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. Results: The seroprevalence against A. phagocytophilum was 8.6% and 6 horses (6.4%) showed positive nested-PCR results. The sequences had 99.5 and 100% homology with the BLAST published A. phagocytophilum amplicons. Conclusion: To the best of our knowledge, this is the first molecular data of A. phagocytophilum in horses in Muş province of Turkey. This study also reveals that A. phagocytophilum isolates isolated from a small region are closely related to A. phagocytophilum isolated from a wide variety of hosts.

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Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe

Cited by 11 publications

References 25 publications

Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR

Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR

Survey of Zoonotic and Non-zoonotic Vector-Borne Pathogens in Military Horses in Lisbon, Portugal

Türkiye’nin Muş İlinde Atlarda Anaplasma phagocytophilum’un İlk Moleküler Tespiti ve Filogenetik Analizi

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