Comparative analysis of 10 small molecules binding to carbonic anhydrase II by different investigators using Biacore technology

Bulk and surface sensitivity of a resonant waveguide grating imager

“…The binding data were found to fit well with a 1:1 binding model with mass transport using Biacore BiaEvalution software, leading to an on-rate constant (k on ) of 2.08 Â 10 6 M À1 s À1 , an off-rate constant (k off ) of 0.026 s À1 , and a dissociation constant (K D ) of 0.013 lM, which are consistent with literature values (k on : 3.0 6 2.1 Â 10 6 M À1 s À1 ; k off : 0.079 6 0.031 s À1 ; K D : 0.031 6 0.011 lM). 26 The binding data obtained confirm that under optimal condition the imager can resolve wavelength shifts down to 0.25 pm with a low thermal noise, and the signal of 0.5 pm can be readily detected. Using these results and Eq.…”

Backscattering Interferometry for Low Sample Consumption Molecular Interaction Screening

“…The ability of BSI to perform such measurements without any modifications illustrates its adaptability. These studies mirrored those performed by SPR 11,12 of this wellcharacterized enzyme-inhibitor system. Figure 4 depicts the binding curve for CAII with benzene sulfonamide and is quite representative for all the inhibitor species studied.…”

“…From this assay, we determined K D to be 0.57 AE 0.07 mM compared with the value reported in the literature of 0.80 AE 0.28 mM. 11,12 Example II Recognizing that a need exists for improved diagnostic methods, we have forged a collaboration with the sexually transmitted disease division of the Centers for Disease Control and Prevention (CDC). Through this association, we have begun to study the potential for BSI to be used for disease diagnostics.…”

Complementary Use of Label-Free Real-Time Biosensors in Drug Discovery of Monoclonal Antibodies

“…Therefore, one way to study the CGRP/4901 interaction was to allow the solution peptides to bind amine-coupled antibody. None of the peptides could be detected directly on the Octet due to their binding signals being within instrument noise, but all were clearly visible on the SPR platforms; small molecule analysis is routine on the Biacore 3000 (Navratilova et al, 2007;Papalia et al, 2006;Cannon et al, 2004;Myszka et al, 2003) and was first described on the ProteOn by Bravman et al, 2006. These authors introduced a method called "one-shot kinetics" that exploits the parallel injection mode of the ProteOn to deliver in a single step a six-membered dilution series of the analyte over six surfaces of immobilized ligand with varying binding capacities.…”

“…Determining whether there is consensus across different biosensor platforms operated by independent users has been a theme of several benchmark studies (Rich et al, 2009;Navratilova et al, 2007;Katsamba et al, 2006;Papalia et al, 2006;Cannon et al, 2004;Myszka et al, 2003). The largest and most recent of these engaged 150 participants from 20 countries who used instruments from ten different manufacturers.…”