Comparative analysis and characterization of the soybean sudden death syndrome pathogen<i>Fusarium virguliforme</i>in the northern United States

Malvick, Dean; Bussey, Karen

doi:10.1080/07060660809507544

Cited by 23 publications

(23 citation statements)

References 28 publications

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“…In previous studies, Walssl exhibited consistent pathogenicity on soybean and, thus, was chosen for determining the host range of F. virgtdiforme (19). Fifteen species of crops, prairie p!ants, or weeds common to the northem regions of the Midwestem United States were eva!uated as potentia!…”

Section: Methodsmentioning

confidence: 99%

“…To produce inoculum, sorghum seed was placed in 250-ml flasks with water and allowed to soak approximately 24 h. Then, the water was drained and the seed autoclaved twice and inoculated with 12 1-cm^ plugs of the plate culture per flask (19,22). The flasks were incubated for 2 to 3 weeks at 23 °C under ambient fluorescent light with a 10-to 11-h photoperiod.…”

Section: Methodsmentioning

confidence: 99%

“…For further confirmation of identity, the internal transcribed spacer (ITS) region of rDNA from five isolates was amplified with ITSl and ITS4 primers, purified, and sequenced at the University of Minnesota BioMedical Genotnics Center (St. Paul) (19). For further confirmation of identity, the internal transcribed spacer (ITS) region of rDNA from five isolates was amplified with ITSl and ITS4 primers, purified, and sequenced at the University of Minnesota BioMedical Genotnics Center (St. Paul) (19).…”

Section: Methodsmentioning

confidence: 99%

“…DNA was extracted from 100 mg of ground root tissue, with the exception of com, where 66 mg was used (19,20). Root tissue collected from each pot was combined and ground for extraction, with the exception of fine root systems, where the roots from multiple pots were aggregated (e.g., Canadian milk vetch, alfalfa, clovers, and other root systems that yielded linaited tissue).…”

Section: Methodsmentioning

confidence: 99%

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Symptomatic and Asymptomatic Host Range of Fusarium virguliforme, the Causal Agent of Soybean Sudden Death Syndrome

et al. 2012

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Kolander, T. M., Bienapfl, J. C, Kude, I E., and Ma!vic!{, D. K. 2012. Symptomatic and asymptomatic host range of Fusarium virguliforme, the causa! agent of soybean sudden death syndrome. Plant Dis. 96:1148-1153.Sudden death syndrome, caused by Fusarium virguliforme, is an important disease of soybean in the United States. Fifteen species of crops, weeds, or prairie plants were evaluated for tbeir potential as bosts of F, virguliforme. Root and foüar symptoms and ptant biomass were assessed foüowing greenhouse inocu!ation studies. Root co!onization of F, virguliforme was determined with isolations and with polymerase chain reaction assays. Soybean, a!fa!fa, pinto and navy bean, white and red c!over, pea, and Canadian mi!!( vetch deve!oped root necrosis. Soybean, alfalfa, and red clover also deve!oped foüar symptoms foUowing inoculation. Sugar beet and canola did not de-Corresponding aut!ior: D. K. Malvick,

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Symptomatic and Asymptomatic Host Range of Fusarium virguliforme, the Causal Agent of Soybean Sudden Death Syndrome

et al. 2012

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“…solani can survive for several years by introducing its spores and mycelium into the soil and into soybean plants (Aoki et al, 2003). An SDS outbreak may occur after fungal mycelia become established and well-adapted to a new, favorable environment (Malvic and Bussey, 2008). In Korea, the soybean SDS occurrence has been increasingly surveyed, however, no studies prioritize the SDS evaluation.…”

Section: Discussionmentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF Fusarium solani CAUSING SOYBEAN SUDDEN DEATH SYNDROME IN KOREA

Lestari¹,

Sun²,

Son³

et al. 2016

J microb biotech food sci

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Association mapping and genomic prediction for resistance to sudden death syndrome in early maturing soybean germplasm

et al. 2015

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Sudden death syndrome (SDS), caused by Fusarium virguliforme, has spread to northern soybean growing regions in the US causing significant yield losses. The objectives of this study were to identify loci underlying variation in plant responses to SDS through association mapping (AM) and to assess prediction accuracy of genomic selection (GS) in a panel of early maturing soybean germplasm. A set of 282 soybean breeding lines was selected from the University of Minnesota soybean breeding program and then genotyped using a genome-wide panel of 1536 single-nucleotide polymorphism markers. Four resistance traits, root lesion severity (RLS), foliar symptom severity (FSS), root retention (RR), and dry matter reduction (DMR), were evaluated using soil inoculation in the greenhouse. AM identified significant peaks in genomic regions of known SDS resistance quantitative trait loci cqSDS001, cqRfs4, and SDS11-2. Additionally, two novel loci, one on chromosome 3 and another on chromosome 18, were tentatively identified. A ninefold cross-validation scheme was used to assess the prediction accuracy of GS for SDS resistance. The prediction accuracy of single-trait GS (ST-GS) was 0.64 for RLS, but less than 0.30 for RR, DMR, and FSS. Compared to ST-GS, none of multi-trait GS (MT-GS) models significantly improved the prediction accuracy due to weak correlations between the four traits. This study suggests both AM and GS hold promise for implementation in genetic improvement of SDS resistance in existing soybean breeding programs.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0324-3) contains supplementary material, which is available to authorized users.

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Comparative analysis and characterization of the soybean sudden death syndrome pathogenFusarium virguliformein the northern United States

Cited by 23 publications

References 28 publications

Symptomatic and Asymptomatic Host Range of Fusarium virguliforme, the Causal Agent of Soybean Sudden Death Syndrome

Symptomatic and Asymptomatic Host Range of Fusarium virguliforme, the Causal Agent of Soybean Sudden Death Syndrome

ISOLATION AND CHARACTERIZATION OF Fusarium solani CAUSING SOYBEAN SUDDEN DEATH SYNDROME IN KOREA

Association mapping and genomic prediction for resistance to sudden death syndrome in early maturing soybean germplasm

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