Comparative analyses reveal potential uses of Brachypodium distachyonas a model for cold stress responses in temperate grasses

Li, Chuan; Rudi, Heidi; Stockinger, Eric J.; Cheng, Hongmei; Cao, Mengji; Fox, Samuel E.; Mockler, Todd C.; Westereng, Bjørge; Fjellheim, Siri; Rognli, Odd Arne; Sandve, Simen Rød

doi:10.1186/1471-2229-12-65

Cited by 59 publications

(81 citation statements)

References 74 publications

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“…8), similar to the patterns observed in Figure 7A and Supplemental Figure S12D. In addition, we analyzed VRN2L levels from a recently published microarray data set (Li et al, 2012), where Bd21 was treated with different periods of cold ranging from 0 to 24 hours. In this data set, VRN2L levels also increased during short cold, peaking between 10 and 24 h of cold treatment.…”

Section: Hvvrn2 and Tmvrn2 (Zinc Finger-cct1 [Zcct1]/ Zcct2)mentioning

confidence: 91%

Interaction of Photoperiod and Vernalization Determines Flowering Time of Brachypodium distachyon

et al. 2013

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Timing of flowering is key to the reproductive success of many plants. In temperate climates, flowering is often coordinated with seasonal environmental cues such as temperature and photoperiod. Vernalization is an example of temperature influencing the timing of flowering and is defined as the process by which a prolonged exposure to the cold of winter results in competence to flower during the following spring. In cereals, three genes (VERNALIZATION1 [VRN1], VRN2, and FLOWERING LOCUS T [FT]) have been identified that influence the vernalization requirement and are thought to form a regulatory loop to control the timing of flowering. Here, we characterize natural variation in the vernalization and photoperiod responses in Brachypodium distachyon, a small temperate grass related to wheat (Triticum aestivum) and barley (Hordeum vulgare). Brachypodium spp. accessions display a wide range of flowering responses to different photoperiods and lengths of vernalization. In addition, we characterize the expression patterns of the closest homologs of VRN1, VRN2 (VRN2-like [BdVRN2L]), and FT before, during, and after cold exposure as well as in different photoperiods. FT messenger RNA levels generally correlate with flowering time among accessions grown in different photoperiods, and FT is more highly expressed in vernalized plants after cold. VRN1 is induced by cold in leaves and remains high following vernalization. Plants overexpressing VRN1 or FT flower rapidly in the absence of vernalization, and plants overexpressing VRN1 exhibit lower BdVRN2L levels. Interestingly, BdVRN2L is induced during cold, which is a difference in the behavior of BdVRN2L compared with wheat VRN2 during cold.

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Section: Hvvrn2 and Tmvrn2 (Zinc Finger-cct1 [Zcct1]/ Zcct2)mentioning

confidence: 91%

Interaction of Photoperiod and Vernalization Determines Flowering Time of Brachypodium distachyon

et al. 2013

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“…Although lowering the water potential of cells is a frequent companion to freezing tolerance (and dormancy), accumulation of a range of solutes such as proline, fructans and mannitol, and proteins such as the ice recrystallization inhibition proteins (IRIPs) [76,77] can be important as well. Cold-responsive IRIPs have been documented in the genome of the model grass Brachypodium [78], but have yet to be reported from a temperate warm-season C 4 grass. A preliminary search of the switchgrass genome using a Lolium perenne IRIP protein sequence (GenBank: ACN38303.1) did not yield a good match.…”

Section: Uncoupling Dormancy From Winter-survival In Switchgrassmentioning

confidence: 99%

Senescence, dormancy and tillering in perennial C4 grasses

2014

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a b s t r a c tPerennial, temperate, C 4 grasses, such as switchgrass and miscanthus have been tabbed as sources of herbaceous biomass for the production of green fuels and chemicals based on a number of positive agronomic traits. Although there is important literature on the management of these species for biomass production on marginal lands, numerous aspects of their biology are as yet unexplored at the molecular level. Perenniality, a key agronomic trait, is a function of plant dormancy and winter survival of the belowground parts of the plants. These include the crowns, rhizomes and meristems that will produce tillers. Maintaining meristem viability is critical for the continued survival of the plants. Plant tillers emerge from the dormant crown and rhizome meristems at the start of the growing period in the spring, progress through a phase of vegetative growth, followed by flowering and eventually undergo senescence. There is nutrient mobilization from the aerial portions of the plant to the crowns and rhizomes during tiller senescence. Signals arising from the shoots and from the environment can be expected to be integrated as the plants enter into dormancy. Plant senescence and dormancy have been well studied in several dicot species and offer a potential framework to understand these processes in temperate C 4 perennial grasses. The availability of latitudinally adapted populations for switchgrass presents an opportunity to dissect molecular mechanisms that can impact senescence, dormancy and winter survival. Given the large increase in genomic and other resources for switchgrass, it is anticipated that projected molecular studies with switchgrass will have a broader impact on related species.

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“…However, Li et al (2012) reported that Brachypodium distachyon could be an important model to investigate the molecular mechanism of low-temperature tolerance in core Pooideae species since B. distachyon have coldresponsive IRIP. Moreover, a large C repeating binding factor genes CBF3 subfamily was identified in B. distachyon.…”

Section: Cold Acclimation and Frost Stress Tolerancementioning

confidence: 99%