Comparative analyses of the neurobehavioral, molecular, and enzymatic effects of organophosphates on embryo-larval zebrafish (Danio rerio)

Schmitt, Cassandra; McManus, Michelle; Kumar, Naveen; Awoyemi, Olushola M.; Crago, Jordan

doi:10.1016/j.ntt.2019.04.002

Cited by 36 publications

(27 citation statements)

References 63 publications

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“…However, no significant changes were detected in the expression levels of the mRNA Ache transcripts and proteins, such as was detected by q‐PCR, ISH, and immunochemical approaches, in Senegalese sole exposed to malathion. Similar results were detected by Schmitt et al 58 in embryo‐larval stages of the zebrafish, Danio rerio , under malathion exposure. Although an inverse correlation between gene expression and enzyme activity has been hypothesized, 59 an absence of a relationship between the two biological endpoints has been increasingly reported in the literature 60–62 .…”

Section: Discussionsupporting

confidence: 89%

Toxicity of malathion during Senegalese sole, Solea senegalensis larval development and metamorphosis: Histopathological disorders and effects on type B esterases and CYP1A enzymatic systems

Ortiz-Delgado

Funes

Albendín

et al. 2021

Environmental Toxicology

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The toxicity of malathion to Solea senegalensis was studied in a static renewal bioassay during its first month of larval life (between 4 and 30 dph). Through the use of different biomarkers and biochemical, cellular and molecular approaches (inhibition of cholinesterases [ChEs], changes in cytochrome P450‐1A [CYP1A] and the study of histopathological alterations), the effects of three concentrations of malathion (1.56, 3.12, and 6.25 μg/L) have been analyzed. In subacute exposure, malathion inhibited cholinesterase activities (AChE, BChE, CbE) in a dose‐ and time‐dependent manner, ranging the inhibition percentage from 20% to 90%. However, the expression levels of CYP1A and AChE transcripts or proteins were not modified. Additionally, exposure to malathion provoked histopathological alterations in several organ systems of Senegalese sole in a time‐ and dose dependent way, namely disruption of parenchymal architecture in the liver, epithelial desquamation, pyknotic nuclei and steatosis in the intestine, disorganization of supporting cartilage, and sings of hyperplasia and hypertrophy in the gills and degeneration of the epithelial cells from the renal tubules. Malathion exposure also provoked strong disorganization of cardiac fibers from the heart. The findings provide evidence that exposure to sublethal concentrations of malathion that provoked serious injury to the fish S. senegalensis, were below the expected environmental concentrations reported in many other ecosystems and different fish species,revealing a higher sensitivity for Solea senegalensis to malathion exposure, thus reinforcing its use as sentinel species for environmental pollution in coastal and estuarine environments.

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Section: Discussionsupporting

confidence: 89%

Toxicity of malathion during Senegalese sole, Solea senegalensis larval development and metamorphosis: Histopathological disorders and effects on type B esterases and CYP1A enzymatic systems

Ortiz-Delgado

Funes

Albendín

et al. 2021

Environmental Toxicology

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“…Deformities, such as growth inhibition, edema formation, and craniofacial and spinal deformities observed in OPPtreated fish [19][20][21], may not be a consequence of AChE inhibition alone. A comparison of molecular, neurobehavioral, and biochemical responses to OPPs suggests that AChE may be an insignificant biomarker for OPP toxicity in ZF [24]. On the other hand, a wide range of OPP-induced toxic mechanisms, including disruption of neurotransmitter metabolism, induction of oxidative stress, muscle exhaustion, calcium dysregulation, and induction of immunological and inflammatory responses, have been proposed in ZF [25,26].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in Danio rerio

Vasamsetti

Chon

Kim

et al. 2021

Genes

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Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibition. To understand the overall molecular mechanisms involved in OPP toxicity, we used the zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed developmental abnormalities and performed whole transcriptome analysis. Phenotypic abnormalities, such as bradycardia, spine curvature, and growth retardation, were observed in phosmet-treated ZF (PTZF). Whole transcriptome analysis revealed 2190 differentially expressed genes (DEGs), with 822 and 1368 significantly up-and downregulated genes, respectively. System process and sensory and visual perception were among the top biological pathways affected by phosmet toxicity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of metabolic pathways, calcium signaling pathway, regulation of actin cytoskeleton, cardiac muscle contraction, drug metabolism–other enzymes, and phototransduction. Quantitative real-time PCR results of six DEGs agreed with the sequencing data expression profile trend. Our findings provide insights into the consequences of phosmet exposure in ZF, as well as an estimate of the potential risk of OPPs to off-target species.

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“…This type of essay allows us to elucidate the mechanism of action of many substances. Many procedures to perform the biochemical assays developed for other species ( Ellman et al, 1961 ; Misra and Fridovich, 1972 ; Bradford, 1976 ; Aebi, 1984 ; Draper and Hadley, 1990 ; LeBel et al, 1992 ; Frasco and Guilhermino, 2002 ) has been adapted and used in zebrafish with great results ( Jin et al, 2015 ; Abe et al, 2018 ; Agostini et al, 2018 ; Maharajan et al, 2018 ; Sarasamma et al, 2018 , 2019 ; Bernardo et al, 2019 ; Mocelin et al, 2019 ; Schmitt et al, 2019 ; Ünal et al, 2019 ; Walpitagama et al, 2019 ).…”

Section: Evaluation Of Sars-cov-2 Neurologic Alterations Through Zebrafish’s Behaviormentioning

confidence: 99%

“…For instance, some tests allow the determination of the reactive oxygen species, the lipid peroxidation, and the activity of the enzymes involved in antioxidant mechanisms, such as superoxide dismutase, catalase, and glutathione-S-transferase ( Diniz et al, 2015 ; Jin et al, 2015 ; Sarasamma et al, 2018 ). Besides, some assays enable the measurement of distinct neurotransmitters activity, such as the choline, GABA, glutamate, glycine, dopamine, and 5-hydroxytryptamine ( Abe et al, 2018 ; Agostini et al, 2018 ; Sarasamma et al, 2018 , 2019 ; Schmitt et al, 2019 ; Walpitagama et al, 2019 ).…”

Section: Evaluation Of Sars-cov-2 Neurologic Alterations Through Zebrafish’s Behaviormentioning

confidence: 99%

“…This type of essay allows us to elucidate the mechanism of action of many substances. Many procedures to perform the biochemical assays developed for other species (Aebi, 1984;Bradford, 1976;Draper and Hadley, 1990;Ellman et al, 1961;Frasco and Guilhermino, 2002;LeBel et al, 1992;Misra and Fridovich, 1972) has been adapted and used in zebrafish with great results (Abe et al, 2018;Agostini et al, 2018;Bernardo et al, 2019;Jin et al, 2015;Maharajan et al, 2018;Mocelin et al, 2019;Sarasamma et al, 2019Sarasamma et al, , 2018Schmitt et al, 2019;Ünal et al, 2019;Walpitagama et al, 2019).…”

Section: Molecular and Biochemical Analysis In The Zebrafish Modelmentioning

confidence: 99%

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Zebrafish as a Translational Model: An Experimental Alternative to Study the Mechanisms Involved in Anosmia and Possible Neurodegenerative Aspects of COVID-19?

Costa

Brigante

Fernandes

et al. 2021

eNeuro

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Comparative analyses of the neurobehavioral, molecular, and enzymatic effects of organophosphates on embryo-larval zebrafish (Danio rerio)

Cited by 36 publications

References 63 publications

Toxicity of malathion during Senegalese sole, Solea senegalensis larval development and metamorphosis: Histopathological disorders and effects on type B esterases and CYP1A enzymatic systems

Toxicity of malathion during Senegalese sole, Solea senegalensis larval development and metamorphosis: Histopathological disorders and effects on type B esterases and CYP1A enzymatic systems

Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in Danio rerio

Zebrafish as a Translational Model: An Experimental Alternative to Study the Mechanisms Involved in Anosmia and Possible Neurodegenerative Aspects of COVID-19?

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