Comparative analyses of biofilm formation among different Cutibacterium acnes isolates

Kuehnast, Torben; Çakar, Fatih; Weinhäupl, Theresa; Pilz, Andreas; Selak, Sanja; Schmidt, M. Alexander; Rüter, Christian; Schild, Stefan

doi:10.1016/j.ijmm.2018.09.005

Cited by 58 publications

(68 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, specific mechanisms employed by C. acnes that would allow the formation and maturation of a biofilm have not been investigated in detail. Kuehnast et al proposed that there might be a correlation between biofilm formation and the C. acnes phylotype, rather than the anatomical site of infection (Kuehnast et al, 2018). The study showed that strains belonging to phylotype IA 1 formed higher amounts of biofilm in microtiter plates than other phylotypes.…”

Section: Biofilmmentioning

confidence: 90%

“…However, C. acnes seems not to be a dedicated, specialized biofilm-former, i.e., it does not use a specific program to form a differentiated biofilm, like it is seen in other species such as ica-positive staphylococci. Indeed, compared to other biofilm-forming bacteria, C. acnes biofilms are rather weak and easily to dissolve, at least in vitro (Kuehnast et al, 2018). Observed differences in biofilm formation in different studies may, at least in part, be related to variations in the employed biofilm detection method and materials used rather than represent real biological variation (Holmberg et al, 2009).…”

Section: Biofilmmentioning

confidence: 99%

See 1 more Smart Citation

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

et al. 2021

View full text Add to dashboard Cite

The bacterial species Cutibacterium acnes (formerly known as Propionibacterium acnes) is tightly associated with humans. It is the dominant bacterium in sebaceous regions of the human skin, where it preferentially colonizes the pilosebaceous unit. Multiple strains of C. acnes that belong to phylogenetically distinct types can co-exist. In this review we summarize and discuss the current knowledge of C. acnes regarding bacterial properties and traits that allow host colonization and play major roles in host-bacterium interactions and also regarding the host responses that C. acnes can trigger. These responses can have beneficial or detrimental consequences for the host. In the first part of the review, we highlight and critically review disease associations of C. acnes, in particular acne vulgaris, implant-associated infections and native infections. Here, we also analyse the current evidence for a direct or indirect role of a C. acnes-related dysbiosis in disease development or progression, i.e., reduced C. acnes strain diversity and/or the predominance of a certain phylotype. In the second part of the review, we highlight historical and recent findings demonstrating beneficial aspects of colonization by C. acnes such as colonization resistance, immune system interactions, and oxidant protection, and discuss the molecular mechanisms behind these effects. This new insight led to efforts in skin microbiota manipulation, such as the use of C. acnes strains as probiotic options to treat skin disorders.

show abstract

Section: Biofilmmentioning

confidence: 90%

Section: Biofilmmentioning

confidence: 99%

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Biofilm formation varies between different bacterial phylotypes, ATCC 6919 has been classified as type IA 1 . This phylotype can produce higher amounts of biofilm biomass, possibly contributing to reduced efficacy of tetracycline (Kuehnast et al, 2018).…”

Section: Biofilm Prevention and Disruptionmentioning

confidence: 99%

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

et al. 2020

View full text Add to dashboard Cite

The Gram-positive bacterium Cutibacterium acnes (previously Propionibacterium acnes), plays an important role in the pathogenesis and progression of the dermatological skin disorder acne vulgaris. The methanolic extract of Helichrysum odoratissimum (L.) Sweet (HO-MeOH) was investigated for its ability to target bacterial growth and pathogenic virulence factors associated with acne progression. The gas chromatography-mass spectrometry (GC-MS) analysis of HO-MeOH identified a-humulene (3.94%), acurcumene (3.74%), and caryophyllene (8.12%) as major constituents, which correlated with previous reports of other Helichrysum species. The HO-MeOH extract exhibited potent antimicrobial activity against C. acnes (ATCC 6919) with a minimum inhibitory concentration (MIC) of 7.81 µg/ml. It enhanced the antimicrobial activity of benzoyl peroxide (BPO). The extract showed high specificity against C. acnes cell aggregation at sub-inhibitory concentrations, preventing biofilm formation. Mature C. acnes biofilms were disrupted at a sub-inhibitory concentration of 3.91 µg/ml. At 100 µg/ml, HO-MeOH reduced interleukin-1a (IL-1a) cytokine levels in C. acnes-induced human keratinocytes (HaCaT) by 11.08%, highlighting its potential as a comedolytic agent for the treatment of comedonal acne. The extract exhibited a 50% inhibitory concentration (IC 50 ) of 157.50 µg/ ml against lipase enzyme activity, an enzyme responsible for sebum degradation, ultimately causing inflammation. The extract's anti-inflammatory activity was tested against various targets associated with inflammatory activation by the bacterium. The extract inhibited pro-inflammatory cytokine levels of IL-8 by 48.31% when compared to C. acnes-induced HaCaT cells at 7.81 µg/ml. It exhibited cyclooxygenase-II (COX-II) enzyme inhibition with an IC 50 of 22.87 µg/ml. Intracellular nitric oxide (NO) was inhibited by 40.39% at 7.81 µg/ml when compared with NO production in lipopolysaccharide (LPS)induced RAW264.7 cells. The intracellular NO inhibition was potentially due to the 2.14 fold reduction of inducible nitric oxide synthase (iNOS) gene expression. The HO-MeOH extract exhibited an IC 50 of 145.45 µg/ml against virulent hyaluronidase enzyme activity, Frontiers in Pharmacology | www.frontiersin.org January 2020 | Volume 10 | Article 1559 1 which is responsible for hyaluronan degradation and scar formation. This study provides scientific validation for the traditional use of H. odoratissimum as an ointment for pimples, not only due to its ability to control C. acnes proliferation but also due to its inhibitory activity on various targets associated with bacterial virulence leading to acne progression.

show abstract

“…Since the discovery that C. acnes is capable of producing biofilm, biofilm has been recognized as an important C. acnes virulence factor 21‐23 . The ability to form biofilms varies among C. acnes isolates, with more invasive isolates being associated with increased biofilm production 24‐25 . Cutibacterium acnes biofilms, while detected to some degree in normal skin, are present in significantly greater frequency in the sebaceous follicles of acne patients, suggesting that C. acnes biofilm production is involved in the pathogenesis of acne 12‐13 .…”

Section: Discussionmentioning

confidence: 99%

Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris

et al. 2020

View full text Add to dashboard Cite

Background The ability of Cutibacterium acnes strains to form biofilms has been correlated with their virulence. Objective This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions. Methods Thin sections of punch biopsy specimens of (i) uninflamed comedones, (ii) inflammatory lesions, and (iii) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis. Results Of the 39 skin specimens from patients with mild‐moderate acne (n = 13) that were studied, nine (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ± 12.242%) compared to uninvolved skin (0.073% ± 0.185%, P = 0.024). Conclusions The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.

show abstract

Comparative analyses of biofilm formation among different Cutibacterium acnes isolates

Cited by 58 publications

References 33 publications

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles of Cutibacterium acnes

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris

Contact Info

Product

Resources

About