Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK

Hardy, Michele E.; Woode, G. N.; Xu, Zhichang; Gorziglia, Mario

doi:10.1128/jvi.65.10.5535-5538.1991

Cited by 29 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results from our study also support recent reports indicating the existence of at least 3 distinct gene segment 4 among BRV in the United States (Hardy et al, 1992;Parwani et al, 1993). Since gene segment 4 of strain B641 has been shown to have a high degree of sequence homology with that of strain UK (Hardy et al, 1991 ), BRV strains UK, B641 and NS-1 most likely belong to the same Ptype.…”

Section: Discussionsupporting

confidence: 90%

“…However, one study indicates 3 different BRV P-types in the United States, with P5 being the most prevalent . Reference strains for each P-and Gtypes known to infect cattle include strains NCDV-Lincoln (PI:G6), United Kingdom (UK) and B641 (P5:G6), and B223 (P11:G10) (Woode et al, 1983;Zheng et al, 1989;Matsuda et al, 1990;Hardy et al, 1991, Hardy et al, 1992.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serological and genotypic characterization of group a rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus

Duhamel

Benfield

et al. 1994

Veterinary Microbiology

View full text Add to dashboard Cite

Two strains of bovine rotavirus (BRV), designated strain Nebraska Scottsbluff-1 (NS-1) and NS-2, were isolated from 2 neighboring cow-calf beef cattle ranches where dams had been vaccinated with a commercial vaccine containing group A BRV strain Neonatal Calf Diarrhea Virus (NCDV)-Lincoln (P1:G6). Northern blot hybridizations using whole genomic RNA probes indicated that strains NS-1 and NS-2 had identical group A RNA electrophoretic patterns and were homologous at all gene segments. Strain NS-1 was compared with reference group A BRV strains using serological and genotypic methods. In vitro virus neutralization assays indicated that strain NS-1 was neutralized by a G6-specific neutralizing monoclonal antibody (mAb) and guinea pig hyperimmune serum (GPHS) raised against BRV strain B641 (P5:G6), but not by G10-specific neutralizing mAb or GPHS raised against BRV strain B223 (P11:G10). Nucleic acid hybridization experiments using whole-genomic RNA probes revealed that gene segment 4 of strain NS-1 differed from BRV strains NCDV-Lincoln and B223, but hybridized with strain B641. Conversely, gene segment 5 of strain NS-1 hybridized with BRV strain B223, but not with BRV strains NCDV-Lincoln and B641. A G-specific cDNA probe produced by reverse transcription polymerase chain reaction (RT-PCR) amplification of strain NS-1 hybridized specifically only with G6 strains NCDV-Lincoln and B641, but not with G10 strain B223. Co-electrophoresis experiments using strains NS-1, B641, and B223 further confirmed these results, suggesting that strain NS-1 was a naturally-occurring reassortant BRV between strains B641 and B223. Taken together these results indicated that a naturally-occurring group A BRV reassortant with a P gene different from the vaccine virus was responsible for the diarrheal syndrome observed on both ranches. Results from this study also indicate the existence of at least 2 different gene segments 5 among group A BRV infecting cattle.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Serological and genotypic characterization of group a rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus

Duhamel

Benfield

et al. 1994

Veterinary Microbiology

View full text Add to dashboard Cite

show abstract

“…Although, research efforts have appeared promising, vaccine field trials continue to produce inadequate results or fail entirely (de Leeuw et al, 1980;Myers and Snodgrass, 1982;Snodgrass et al, 1982;Waltner-Toews et al, 1985;Yuan et al, 1998). The inadequacy of rotavirus vaccines may be explained, in part, by the complex antigenic diversity of the group A rotaviruses (Woode et al, , 1987Zheng et al, 1989;Hardy et al, 1991;Parwani et al, 1993;, and by doubts concerning which of the two main antigens, VP4 or VP7, is the most important antigen to induce immune protection (Ward et al, 1993;. In addition, immunizing neonates with modified live rotavirus or coronavirus vaccine is difficult because of interference by colostral antibodies which contain neutralizing activity and effectively block the host's immune response (Waltner-Toews et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

In vitro studies on the use of clay, clay minerals and charcoal to adsorb bovine rotavirus and bovine coronavirus

Clark¹,

Sarr²,

Grant³

et al. 1998

Veterinary Microbiology

View full text Add to dashboard Cite

Rotaviruses are the leading cause and coronaviruses are the major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling infection, we have investigated broad spectrum antiviral agents that are not discriminatory among various viruses. This report involves testing a variety of adsorbent agents including charcoal, clay, and clay minerals to adsorb rotavirus and coronavirus in vitro. Results revealed that all the adsorbent agents had good to excellent capability of adsorbing rotavirus and excellent capability of adsorbing coronavirus. Percent adsorptions ranged from 78.74% to 99.89% for rotavirus and 99.99% for coronavirus; while sand (negative control) was < 0.01%. A high affinity binding was present as determined by a low percent desorption (0.06-3.09%). However, the adsorbent bound virus complex retained, and may have actually enhanced, infectivity.

show abstract

“…ADRV and IDIR VP4 proteins are likely to represent vastly different VP4 serotypes of group B rotavirus. VP4 amino acid homologies between neonatal calf diarrhea virus and B641 group A rotavirus proteins have been used to suggest that these strains represent different serotypes (23)(24)(25)). In the case of neonatal calf diarrhea virus and B641, the amino acid sequences were 64% identical in their VP8 protein and 73% identical in all of VP4 (25).…”

mentioning

confidence: 99%

Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV

Mackow

Werner-Eckert

Fay

et al. 1993

J Virol

View full text Add to dashboard Cite

A complete cDNA copy of the fourth RNA segment of the human group B rotavirus adult diarrheal rotavirus (ADRV) has been cloned into lambda phage and excised into plasmid pSK Bluescript. Gene segment 4 contains 2,303 bases and encodes one long open reading frame beginning at base 16 and terminating at base 2263. The encoded protein contains 749 amino acids, with a calculated molecular mass of 84.4 kDa and a pI of 6.1. Gene 4 cDNA was inserted into a recombinant baculovirus via homologous recombination. The gene 4 polypeptide migrates at 84 kDa when expressed either by a recombinant baculovirus or in vitro in a rabbit reticulocyte lysate. The gene 4 protein is immunoprecipitable by hyperimmune serum to ADRV, human ADRV convalescent-phase serum, a porcine group B rotavirus infection serum, and a monoclonal antibody made to ADRV virion. Guinea pig hyperimmune serum to the baculovirus-expressed ADRV VP4 protein recognizes virus and immunoprecipitates an 84-kDa protein from in vitro translations of total ADRV mRNA. In addition, the gene 4-encoded protein shares significant amino acid identity and similarity with the group A rotavirus VP4 protein. This information, together with our previous identification of an 84-kDa protein present on iodinated intact virion but not EDTA-treated ADRV, suggests that gene 4 encodes the VP4 protein equivalent present on the outer capsid of ADRV. The ADRV VP4 protein is also 58% identical to the IDIR rat group B rotavirus gene segment 3 protein. The substantial differences between these two group B VP4 proteins suggests that they are distantly related and likely to define two different group B rotavirus VP4 serotypes. The baculovirus-expressed VP4 protein should be useful for developing serotyping reagents and tests for human and animal group B rotaviruses as well as for addressing the role of VP4 in ADRV neutralization.

show abstract

Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK

Cited by 29 publications

References 31 publications

Serological and genotypic characterization of group a rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus

Serological and genotypic characterization of group a rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus

In vitro studies on the use of clay, clay minerals and charcoal to adsorb bovine rotavirus and bovine coronavirus

Identification and baculovirus expression of the VP4 protein of the human group B rotavirus ADRV

Contact Info

Product

Resources

About