Comparative activities of oxidoreductase enzymes in tissue extracts of crop plants and in culture fluids of fungal mycelia

Gramss, G.; Günther, Th.; Voigt, Kerstin; Kirsche, Brigitta

doi:10.1016/s0045-6535(97)10077-7

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Their redox potential of E 0 = 0.89-0.95 V [18] surpasses that of fungal laccases and especially of plant laccases (E 0 = 0.4 V) and monophenol monooxygenases (MMO, EC 1.14.18.1, E 0 = 0.26 V) [15,30]. Plant POs catalyze one-electron oxidations of phenolics and humic substances [31] …”

Section: Introductionmentioning

confidence: 99%

“…Plant released phenolics acting as aryloxy radicals mediate the formation of Mn 3+ (0.8-0.9 V) [15,32] and a partial oxidation of veratryl alcohol from maize roots (1.4 V) [30]. They expand the substrate range at least in part to that of fungal POs.…”

Section: Introductionmentioning

confidence: 99%

“…In plant roots, the bulk of the PO is localized in the primary cell wall but also in membranes, organelles, vacuoles, and the cytoplasm [33]. Class (iii) peroxidases could thus be leached from roots of cotton, wheat, cress, tomato, water hyacinth, French bean, rice [33,35] and other plants [15,30,36]. Taken with the chromogen guaiacol at pH 7.0, alfalfa plants but not oilseed rape surpassed the root exuded PO activities of white mustard 167-182 times both in axenic and natural soil cultures [15,37].…”

Section: Introductionmentioning

confidence: 99%

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Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

Gramss

2018

Soil Systems

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Abstract:Rhizosphere priming by terrestrial plants comprises increased or repressed efflux of CO 2 and N from soil organic matter (SOM), decaying under the impact of temperature, moisture, and the composition of rhizodeposits. Contemporarily, increases in water solubility vs. losses in molecular size, aromaticity, and the content in phenolic OH groups denote the degradation of SOM in planted soil. Root peroxidases (POs) and 'polyphenoloxidases' are surmised to contribute to these effects, however, final evidence for this is lacking. Therefore, seedlings of white mustard, alfalfa, and oilseed rape with wide spans in PO release were grown in hydroponic cultures at variable levels of Cu/Fe/Mn as Fenton metals, but also under P and Fe starvation to stimulate the release of carboxylic acids that form catalytic Mn 3+ chelants from Mn 2+ and MnO 2 . The shortage in active oxygen as a cosubstrate of POs delayed the immediate oxidation of 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) supplements to the green ABTS •+ by PO/H 2 O 2 , the possible formation of Mn 3+ via PO catalyzed aryloxy radicals from root-released phenolics, and of HO • by metal cations in H 2 O 2 dependent Fenton-like reactions. Enhanced by exuded and external malate, O 2 independent MnO 2 supplements in some treatments formed ABTS •+ spontaneously. The culture fluids then turned red in all treatments within 24-60 h by the formation of azodication (ABTS 2+ ) derivatives in a second plant initiated oxidation step that is known to be catalyzed by substrate radicals. It is concluded that plants initiate oxidative activities that contribute to rhizosphere priming in an environment of oxidoreductase and carboxylate exudates, the indicated presence of mediating substrate radicals, and the cations and (hydr)oxides of transition metals. Pathways of H 2 O 2 production upon the degradation of carboxylates and by the POs themselves are indicated.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

Gramss

2018

Soil Systems

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“…Se desconocen los mecanismos por los que esta especie resiste las altas concentraciones de hidrocarburos en las que se desarrolla, es muy probable que el complejo enzimático CYTP4501A1 esté involucrado en la remoción de hidrocarburos. Se ha comprobado la participación activa de estos complejos enzimáticos en procesos de detoxificación de diferentes organismos, animales, vegetales y hongos (Gramss et al, 1997). Finalmente se conocen antecedentes de la existencia de monoxigenasas en raíces de plantas (Khatisashvili et al, 1997).…”

Section: Planteamiento Del Problemaunclassified

Expresión de la actividad enzimática de citocromo P4501A1 en una planta (Cyperus laxus) fitorremediadora de suelos contaminados con hidrocarburos

López Martínez

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Oxidoreductase enzymes liberated by plant roots and their effects on soil humic material

Gramss¹,

Voigt²,

Kirsche³

1999

Chemosphere

103

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Comparative activities of oxidoreductase enzymes in tissue extracts of crop plants and in culture fluids of fungal mycelia

Cited by 4 publications

References 39 publications

Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

Formation of the Azodication (ABTS2+) from ABTS [2,2′-Azinobis-(3-ethylbenzothiazoline-6-sulphonate)] in Sterile Plant Cultures: Root–Exuded Oxidoreductases Contribute to Rhizosphere Priming

Expresión de la actividad enzimática de citocromo P4501A1 en una planta (Cyperus laxus) fitorremediadora de suelos contaminados con hidrocarburos

Oxidoreductase enzymes liberated by plant roots and their effects on soil humic material

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