Comparaison des tests microtox et daphnie pour l'evaluation de la toxicité aigue d'effluents industriels

Vasseur, Paule; Férard, J. F.; Vial, Jérôme

doi:10.1016/0143-1471(84)90118-1

Cited by 17 publications

(7 citation statements)

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“…These results are not surprising for the Microtox and glucose-mineralization assays, since they used pure bacterial strains grown under standard conditions. The good repeatability of the Microtox test was also confirmed in many studies (Bulich et al, 1981;Bazin etal., 1987;Curtis etal., 1982;Ferard etal., 1983;Qureshi et al, 1984;Reteuna et al, 1986;Vasseur et al, 1984aVasseur et al, , 1984bVasseur et al, , 1986. On the other hand our results for the oxygen consumption assay were better than those observed in the literature (Brown et al, 1981;King & Painter, 1986).…”

Section: Resultscontrasting

confidence: 53%

Performances of three bacterial assays in toxicity assessment

Reteuna¹,

Vasseur²,

Cabridenc

1989

Hydrobiologia

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Three differing bacterial toxicity assays were compared: the 'Microtox' test, (Photobacterium phosphoreum luminescence inhibition assay), the 'oxygen consumption of activated sludge' assay (ISO 8192), and the 'Glucose U-14 C mineralization' assay (the rate of release of 4 CO2 by 'Escherichia col£). Metals, amines, halogenated alcans, chlorophenols, aromatic hydrocarbons, surfactants, and pesticides were screened for their toxic activity.Results showed satisfactory repeatability of the three bacterial assays with variation coefficients between 5 and 32%. The 'Microtox' assay was the most sensitive test evaluated under our conditions. The lower sensitivity of the 'oxygen consumption' assay may have been due to high concentrations of substrates which modify toxicant bioavailability, and also to a high biomass/toxic substances ratio. The 'Glucose U-4 C mineralization' assay was selective, and low in sensitivity; but the specific species used in this test -Escherichia coli -may have been responsible for this selectivity.The 'Microtox' test appears to be well adapted to the detection of aquatic environmental pollution, and to the toxicity screening of complex solid waste effluents and/or leachates. The 'oxygen consumption' assay can be advantageously used to measure the impact of sewage on activated sludge in biological treatment plants. The 'Glucose U-' 4 C mineralization' assay, which does not require high biomass, can be useful for in situ studies using field microorganisms.

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Section: Resultscontrasting

confidence: 53%

Performances of three bacterial assays in toxicity assessment

Reteuna¹,

Vasseur²,

Cabridenc

1989

Hydrobiologia

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“…Moreover, the EC 50 values from that test do not follow the same trends than those from respirometry. Although several authors have found a good agreement between the response to different chemicals of V. fischeri and other microorganisms living in freshwater, such as Ceriodaphnia dubia (Doherty et al 1999), Daphnia magna (Vasseur et al 1984) and Tetrahymena pyriformi (Cronin et al 1991), some of those authors have concluded that for certain groups of compounds the behaviour of V. fischeri does not serve as a good model for toxicity .…”

Section: Resultsmentioning

confidence: 95%

Comparison of experimental methods for determination of toxicity and biodegradability of xenobiotic compounds

et al. 2011

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Different methods for determining the toxicity and biodegradability of hazardous compounds evaluating their susceptibility to biological treatment were studied. Several compounds including chlorophenols and herbicides have been evaluated. Toxicity was analyzed in terms of EC50 and by a simple respirometric procedure based on the OECD Method 209 and by the Microtox® bioassay. The values of EC50 obtained from respirometry were in all the cases higher than those from the Microtox® test. The respirometric inhibition values of chlorophenols were related well with the number of chlorine atoms and their position in the aromatic ring. In general, herbicides showed lower inhibition, being alachlor the less toxic from this criterion. For determination of biodegradability an easier and faster alternative to the OECD Method 301, with a higher biomass to substrate ratio is proposed. When this test was negative, the Zahn-Wellens one was performed in order to evaluate the inherent biodegradability. In the fast test of biodegradability, 4-chlorocatechol and 4-chlorophenol showed a complete biodegradation by an unacclimated sludge upon 48 h. These results together with their low respirometric inhibition, allow concluding that these compounds could be conveniently removed in a WWTP. Alachlor, 2,4-dichlorophenol, 2,4,6-trichlorophenol and MCPA showed a partial biodegradation upon 28 days by the Zahn-Wellens inherent biodegradability test.

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“…plants would be essential. Vasseur and Ferrard 1984 described how the results of Vibrio fischeri and Daphnia tests can differ according to the source of the industrial effluents.…”

Section: Methodsmentioning

confidence: 99%