Comparaison de l'efficacité de deux méthodes de multiplication rapide de plants de bananier à partir de l'étude des caractéristiques agronomiques d'un hybride de bananier plantain (Musaspp.)

Abstract

Comparison of the effectiveness of two vegetative propagation methods for banana trees from the study of the agronomic characteristics of a hybrid of plantain (Musa spp.). Abstract-Introduction. The objective of our study was to evaluate the agronomic performance of plantain hybrid plants (CRBP-39, Musa cv. AAAB) obtained through two vegetative propagation methods: in vitro culture, called the "CIV technique", and a newly developed technique of plants derived from stem bits, called the "PIF technique". The study, carried out in Cameroon, took place at a high altitude (1250 m) and covered one cropping cycle. The CRBP-39 hybrid was chosen because of its resistance to Black Leaf Streak Disease and its agronomic performance. Materials and methods. Vitroplants and plants obtained through the PIF technique developed at CARBAP (Cameroon) were planted separately in elementary plots of 16 plants. No chemical control was administered against Black Leaf Streak Disease. Observations were essentially made on agronomic characteristics. Results and discussion. For the mother crop, there was no significant difference between the plants obtained from one or the other vegetative propagation method concerning their pseudo-stem height and girth; the number of functional leaves at shooting; the duration of the production cycle; the bunch weight; the number of hands and fruits of the bunch; and the length and circumference of the fruit. For the ratoon crop, significant differences were observed concerning the pseudo-stem height and girth, and the number of emitted and functional leaves at harvest. These results globally indicate a strong similarity in vegetative and bunch characteristics of plants propagated with one or the other method. The PIF technique, which is used more and more by small-scale farmers because of its simplicity, rapidness and low cost of production, is a method of vegetative propagation that helps to produce plants similar to tissue cultured plants in terms of agronomic qualities. Cameroon / Musa (plantains) / hybrids / plant propagation / vitroplants / propagation materials Comparaison de l'efficacité de deux méthodes de multiplication rapide de plants de bananier à partir de l'étude des caractéristiques agronomiques d'un hybride de bananier plantain (Musa spp.). Résumé-Introduction. Nos travaux ont eu pour objectif de comparer les performances agronomiques des plants d'un hybride de bananier plantain (CRBP-39, Musa cv. AAAB) obtenus par utilisation de deux méthodes de multiplication végétative : la culture de tissus in vitro (CIV) et une technique de propagation par multiplication de plants à partir de fragments de tiges (dite technique des PIF) développée récemment. L'étude, effectuée au Cameroun, a été menée en altitude (1250 m) ; elle a porté sur un cycle de culture. L'hybride CRBP-39 a été choisi en raison de sa résistance à la maladie des raies noires et de ses performances agronomiques. Matériel et méthodes. Des vitroplants et des plants obtenus par la technique des PIF, technique de propagation horticole ...

The aphidPentalonia nigronervosa(Hemiptera: Aphididae) takes advantage from the quality change in banana plant associated withBanana bunchy top virusinfection

“…Plants were maintained and multiplied in the tropical greenhouse (greenhouse no. 13; G2) using the PIF ( Plants Issus de Fragments de tiges , Plants from Stem Fragments) technique (Kwa 2003, 2009, Meutchieye 2009, Sadom et al 2010, Mbunzu et al 2019). Plants were irrigated daily, until they reached 40–60 days of age (4–6 leaf stage), for their use in all tests.…”

Management practices and incidence of pests in plantain (Musa paradisiaca AAB) crops. Consequences on the sustainability of the cropping systems

“…This result highlights that particular attention should be paid to the sanitary state of the plant material used during plantain plantation for ensuring plantation durability. In this sense, it has been already observed that the use of healthy plants, obtained through the PIF technique (Lefranc et al, 2010;Sadom et al, 2010), significantly reduced pest infestations at the beginning of the plantation (Loranger-Merciris et al, 2022). In contrast, the higher density of H. multicinctus observed in agroecological perennial banana plantations showed that these ectoparasite nematodes are more persistent in more organic cropping systems.…”

Preference of Pentalonia nigronervosa for infected banana plants tends to reverse after Banana buchy top virus acquisition

“…The plants were identi ed and collected from peasant plantations in the province of South Kivu in (Democratic Republic of Congo), with the support of the International Institute of Tropical Agriculture-IITA/Kalambo (Bukavu, DR Congo), in accordance with 39 , then certi ed by the Plant Clinic of the 'Agro Louvain-Services' platform (located in the plant pathology laboratory of the Université catholique de Louvain, Louvain-la-Neuve, Belgium). As transmission of the virus by mechanical inoculation has never been successful 40,41 , all the plants used including the healthy ones were maintained and propagated in the tropical greenhouse (greenhouse n°13; G2) of the UCLouvain (Louvain-la-Neuve, Belgium) using the PIF technique (Plants from Stem Fragments) [42][43][44][45][46] and were irrigated daily, until they reached 40-60 days of age (4-6 leaf stage), for their use in aphid rearing, as well as for viruliferous and non-viruliferous alate aphid attractiveness tests. Severe symptoms of BBTV were observed for plants obtained directly by the PIF technique from infected banana bulbs, since offspring from an infected strain are automatically infected and show severe symptoms of BBTV 47,48 .…”