Comparación entre 2 técnicas de concentración de parásitos (Copropack versus Mini Parasep Solvent Free)

Tenorio-Abreu, A; Gil-Tomás, Jesús J.; Martínez-Macías, Olalla; Colomina-Rodríguez, Javier

doi:10.1016/j.eimc.2012.10.008

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…The Midi Parasep® SF technique has proved to be a simpler, less toxic, rapid, and sensitive concentration technique for parasites in faeces (Adugna et al 2017;Tenorio-Abreu et al 2013). In fact, the technique proved to be more sensitive than FECT in the detection of Strongyloides stercoralis larvae in human faeces (Kopolrat et al 2022).…”

Section: Discussionmentioning

confidence: 99%

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Application of the Midi Parasep® SF technique for the detection of L1 larvae of Angiostrongylus cantonensis in faeces of infected rats

et al. 2023

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We investigated parasitic zoonoses caused by protozoans and helminths in urban and peri-urban rat populations (Rattus norvegicus and R. rattus) in Spanish cities. Rats were trapped and then dissected to remove adult helminths, and the contents of the large intestine were retrieved for the study of parasitic forms. The Midi Parasep® solvent free (SF) technique was used to concentrate the parasites in the intestinal contents. Some of the rats studied (n = 8) were infected by the rat lungworm, Angiostongylus cantonensis, whose first stage larvae (L1) are shed in rat faeces. After the concentration technique, L1 larvae were found in the sediment of 6 of the 8 positive rats. The two negative sediment samples were due to the presence of either only adult females or, in addition to males, only young females in the lungs of the rats. In view of our results, Midi Parasep® SF turned out to be a simple, rapid, inexpensive, and sensitive method to detect nematode larvae, such as the L1 larvae of A. cantonensis (or A. costaricensis), in natural and experimentally infected rats.

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Section: Discussionmentioning

confidence: 99%

“…2017; Tenorio-Abreu et al . 2013). In fact, the technique proved to be more sensitive than FECT in the detection of Strongyloides stercoralis larvae in human faeces (Kopolrat et al .…”

Section: Discussionmentioning

confidence: 99%

Application of the Midi Parasep® SF technique for the detection of L1 larvae of Angiostrongylus cantonensis in faeces of infected rats

et al. 2023

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“…Filter-based faecal parasite concentrators have been available for at least two decades, and differ notably by the filter pore size (Table 2). Several studies have shown that the performance of these kits depends on several parameters, including the type of filter, the volume of stool, and the centrifugation time [4,8,9,11,12]. Some authors have found that solvent-based kits are more efficient than those that do not use solvents [8,9] and those based on filtration [7,11].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Intestinal Parasitoses: Comparison of Two Commercial Methods for Faecal Concentration Using a Polyparasitized Artificial Liquid Stool

Paugam¹,

Ngamada²

2016

Appli Micro Open Access

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As conventional faecal concentration methods for parasite detection may carry health risks due to the toxicity of the solvents used (ether, acetyl-acetate), we compared parasite recovery obtained with the new solvent-free EasyPara® kit (Servisol, Meudon, France), which consists of a single-use tube containing a porosity gradient filter ( 200 to 400 µm), and that obtained with the Para-Selles® system (Fumouze Diagnostics/Sofibel, Levallois-Perret, France), an ethyl-acetate solvent sedimentation method routinely used in our laboratory.Both kits were used as recommended by their manufacturers. Both kits concentrate parasites in a pellet, which is suspended for microscopic examination. Parasites were identified on the basis of their morphology. The numbers of parasites recovered in the total pellets were compared between the kits. To compare the detection thresholds of the kits, we tested a liquid polyparasitized stool sample prepared by pooling clinical parasitized stool samples (protozoa and helminths) from parasitized patients consulting our hospital and stored in our collection and diluting it with saline solution. Using the liquid polyparasitized stool sample, the recovery concentrations for Entamoeba histolytica/dispar, Entamoeba coli and Angystrongyloides stercoralis larvae were significantly different with the two kits but no difference was observed for Giardi intestinalis cysts and Ascaris, tapeworm egg detection.Parasite recovery was better with the EasyPara® kit than with Para-Selles®, probably owing to the presence of an original porosity gradient filter.

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Bioacumulación de coproantígenos de Giardia y Cryptosporidium en un entorno humano infantil

Gutiérrez‐Fernández

Almagro-Nievas²,

Heras-Caña

et al. 2015

Anales de Pediatría

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Comparación entre 2 técnicas de concentración de parásitos (Copropack versus Mini Parasep Solvent Free)

Cited by 3 publications

References 6 publications

Application of the Midi Parasep® SF technique for the detection of L1 larvae of Angiostrongylus cantonensis in faeces of infected rats

Application of the Midi Parasep® SF technique for the detection of L1 larvae of Angiostrongylus cantonensis in faeces of infected rats

Diagnosis of Intestinal Parasitoses: Comparison of Two Commercial Methods for Faecal Concentration Using a Polyparasitized Artificial Liquid Stool

Bioacumulación de coproantígenos de Giardia y Cryptosporidium en un entorno humano infantil

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