Comparação do sistema de identificação automatizado Vitek 2 e PCR-ITS para caracterização das espécies dos isolados clínicos de Candida spp.

Higashi, Carolina Matias; Takashima, Fabiana Hiromi; Rechenchoski, Daniele Zendrini; Stipp-Abe, Aline Tancler; Vespero, Eliana Carolina; Quesada, Regina Mariuza Borsato; Pelisson, Marsileni

doi:10.5433/1679-0367.2015v36n1suplp233

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Although these results showed a concordance rate of only 55% for the three methods, these results suggest that conventional methods are not ideal for the quick and accurate identification of samples from clinical sources, since they consume time and their results are subject to errors, and additional identification tests or repetitions are required (Aubertine et al, 2006;Higashi et al, 2015). Graf et al (2000) evaluated Candida and other yeasts using the Vitek 2-YST system in a total of 241 isolates versus automated prior version identification, myoscopy, and agglutination test for C. krusei.…”

Section: Discussionmentioning

confidence: 89%

Molecular Diagnosis Compared to Conventional Diagnosis for Rapid Detection of Resistant Strains of Candida Spp.

Bastos¹,

Alves²,

Neves³

et al. 2023

RSD

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Yeast Candida spp. can cause serious infections, especially in patients of the hospital environment. Variation in the virulence profile of Candida clinical isolates and high mortality rates indicate the importance of rapid and accurate identification, as well as susceptibility testing for successful candidiasis treatment. A total of 20 strains were isolated from patients from Tertiary Hospital of the Northern Zone of Ceará. PCR amplification followed by agarose gel electrophoresis (PCR-AGE) and the manual method (culturing on CHROMagar-Candida) and VITEK automated method were used to test a total of 20 fungal strains from clinics sources. Five strains of C. albicans, 2 of C. tropicalis and 4 strains of C. parapsilosis which were cultured for the determination of MIC (Minimum Inhibitory Concentration). The best sensitivity rate was demonstrated by automatic identification and PCR-AGE, where all 20 strains isolated with a sensitivity profile of 100% (20/20) were completely identified. According to the data obtained, resistance to amphotericin B (CIM ≥ 1 µg/ml) was observed in some isolates of C. tropicalis and C. parapsilosis. However, for the other antifungals, these same species presented 100% sensitivity (MIC ≤ 0.125 μg / ml; MIC ≤ 8 μg / ml). Isolates of C. albicans were sensitive to all antifungal agents with 100% sensitivity, although 75% of the isolates showed a tendency for less susceptibility to amphotericin B. In this study, we confirmed the efficacy and applicability of PCR-AGE in the identification and differentiation of yeast Candida spp. from clinical samples.

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Section: Discussionmentioning

confidence: 89%

Molecular Diagnosis Compared to Conventional Diagnosis for Rapid Detection of Resistant Strains of Candida Spp.

Bastos¹,

Alves²,

Neves³

et al. 2023

RSD

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“…The results for VITEK  2 Compact system are known to be good, as well as the speed of analysis 43 ; however, wrong results may occur. Thus, the use of molecular analysis is required, leading to a higher cost and the need for specialized labor, and this is not the case in most microbiology laboratories 39 .…”

Section: One Of the Alternatives For Distinguishing Betweenmentioning

confidence: 99%

MALDI-TOF mass spectrometry identification of yeast-form fungi: a comparison between methods

2019

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Introduction: Identification of yeast species has clinical and epidemiological value. Different methods can be used, such as chromogenic media, microculture on corn meal agar with Tween 80, as well as conventional biochemical and automated methods. Recently, proteomic studies employing matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry have been a major advance in diagnosis due to speed of execution and accuracy of results. Methods: For this study, 79 yeast samples were submitted to identification using chromogenic medium, microculture on corn meal-Tween 80 agar, VITEK  2 Compact identification, and MALDI-TOF mass spectrometry. Results: Most of the 79 samples were identified, with differences in the performance of the methods used. Colonial morphology and microscopy were compatible with the genus Candida. MALDI-TOF mass spectrometry had the best performance, with 78 strains identified (98.7%), compared to VITEK  2 Compact (92.4%) and microculture on corn meal agar (70.9%). Conclusions: MALDI-TOF mass spectrometry using the VITEK  MS instrument performed best and has proven to be a revolutionary method in clinical microbiology laboratories. Regarding the identification of C. albicans and C. tropicalis, the chromogenic medium had excellent performance, thus being a good option to optimize the process.

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“…Several commercial products and systems have been developed the aim of solve some difficulties experienced by the clinical microbiology laboratories in the diagnosis of infections caused by Candida species [20], for example Agar containing chromo gens, kits and panels semi or full automated to the presumed or definitive identification of the most prevalent species [21,22]. The use of only phenotypic testing is not highly effective for identification of Candida species because some species has few morphological and biochemical variations, therefore phenotypic and molecular methods can be used in together to increase reliability in the identification of these species.…”

Section: Introductionmentioning

confidence: 99%

Molecular Methods Developed for the Identification and Characterization of Candida Species

Grisolia¹

2017

IJGS

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Candida species are commensally microorganisms in healthy individuals, but is also the most opportunistic fungal pathogen of human. From benign skin-mucosal forms to invasive ones, Candida infection, can compromise various organs systematic and cause diseases in immunocompromised or critically ill patients. Hence the accurate identification and characterization of the disease-causing strains is crucial for diagnosis, clinical treatment and epidemiological studies of candidacies. Molecular methods the identification and characterization support gives in epidemiological research and in development of novel antifungal with specific molecular targets, especially in the case of resistant Candida species. This review aims to describe the main methods used in the identification and characterization the Candida species and discusses future perspectives.

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Comparação do sistema de identificação automatizado Vitek 2 e PCR-ITS para caracterização das espécies dos isolados clínicos de Candida spp.

Cited by 4 publications

References 13 publications

Molecular Diagnosis Compared to Conventional Diagnosis for Rapid Detection of Resistant Strains of Candida Spp.

Molecular Diagnosis Compared to Conventional Diagnosis for Rapid Detection of Resistant Strains of Candida Spp.

MALDI-TOF mass spectrometry identification of yeast-form fungi: a comparison between methods

Molecular Methods Developed for the Identification and Characterization of Candida Species

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