Comparability of two different polyacrylamide gel electrophoresis methods for the classification of LDL pattern type

Bañuls, Celia; Bellod, Lorena; Jover, A; Martínez-Triguero, María L.; Víctor, Víctor M.; Rocha, Milagros; Hernández‐Mijares, Antonio

doi:10.1016/j.cca.2011.09.047

Cited by 24 publications

(15 citation statements)

References 25 publications

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“…There is a strong correlation between size and density of LDL particles analyzed by ultracentrifugation and GGE, respectively; however, these parameters are not identical. Some authors used tube gel electrophoresis for LDL subfraction analysis for rapid acquisition of quantitative results [32, 33]. …”

Section: Ldl Subclasses and Methods Of Their Identificationmentioning

confidence: 99%

Small Dense Low-Density Lipoprotein as Biomarker for Atherosclerotic Diseases

Ivanova

Myasoedova

Melnichenko

et al. 2017

Oxidative Medicine and Cellular Longevity

285

233

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Low-density lipoprotein (LDL) plays a key role in the development and progression of atherosclerosis and cardiovascular disease. LDL consists of several subclasses of particles with different sizes and densities, including large buoyant (lb) and intermediate and small dense (sd) LDLs. It has been well documented that sdLDL has a greater atherogenic potential than that of other LDL subfractions and that sdLDL cholesterol (sdLDL-C) proportion is a better marker for prediction of cardiovascular disease than that of total LDL-C. Circulating sdLDL readily undergoes multiple atherogenic modifications in blood plasma, such as desialylation, glycation, and oxidation, that further increase its atherogenicity. Modified sdLDL is a potent inductor of inflammatory processes associated with cardiovascular disease. Several laboratory methods have been developed for separation of LDL subclasses, and the results obtained by different methods can not be directly compared in most cases. Recently, the development of homogeneous assays facilitated the LDL subfraction analysis making possible large clinical studies evaluating the significance of sdLDL in the development of cardiovascular disease. Further studies are needed to establish guidelines for sdLDL evaluation and correction in clinical practice.

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Section: Ldl Subclasses and Methods Of Their Identificationmentioning

confidence: 99%

Small Dense Low-Density Lipoprotein as Biomarker for Atherosclerotic Diseases

Ivanova

Myasoedova

Melnichenko

et al. 2017

Oxidative Medicine and Cellular Longevity

285

233

View full text Add to dashboard Cite

show abstract

“…After an overnight fast, the subjects received a bolus injection of 60 mol/kg body weight [5,5, H] l-leucine (Cambridge Isotopes, Andover, MA) to determine the kinetics of apoB-100. Blood samples were collected into tubes containing EDTA (0.15%) at baseline (0 h, 8 AM) and at 30 min, 1,2,4,6,8,10,12,14,21,27,33,45,57, and 69 h after the isotope was injected. During the first 48 h of the metabolic study, the subjects were given equal portions of a fat-free energy drink (240 g of Genisoy powder and 850 g strawberry sorbet, 58 g sugar per 1,000 ml) at 10 AM, 1 PM, 4 PM, 7 PM, and 10 PM, to minimize the intermittent influx of intestinal chylomicrons, which might interfere with the kinetics of TRL apoB (20,21).…”

Section: Methodsmentioning

confidence: 99%

Metabolism and proteomics of large and small dense LDL in combined hyperlipidemia: effects of rosuvastatin

Thongtang

Diffenderfer

Ooi

et al. 2017

Journal of Lipid Research

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Small dense LDL (sdLDL) has been reported to be more atherogenic than large buoyant LDL (lbLDL). We examined the metabolism and protein composition of sdLDL and lbLDL in six subjects with combined hyperlipidemia on placebo and rosuvastatin 40 mg/day. ApoB-100 kinetics in triglyceride-rich lipoproteins (TRLs), lbLDL (density [] = 1.019-1.044 g/ml), and sdLDL ( = 1.044-1.063 g/ml) were determined in the fed state by using stable isotope tracers, mass spectrometry, and compartmental modeling. Compared with placebo, rosuvastatin decreased LDL cholesterol and apoB-100 levels in TRL, lbLDL, and sdLDL by significantly increasing the fractional catabolic rate of apoB-100 (TRL, +45%; lbLDL, +131%; and sdLDL, +97%), without a change in production. On placebo, 25% of TRL apoB-100 was catabolized directly, 37% was converted to lbLDL, and 38% went directly to sdLDL; rosuvastatin did not alter these distributions. During both phases, sdLDL apoB-100 was catabolized more slowly than lbLDL apoB-100 ( < 0.01). Proteomic analysis indicated that rosuvastatin decreased apoC-III and apoM content within the density range of lbLDL ( < 0.05). In our view, sdLDL is more atherogenic than lbLDL because of its longer plasma residence time, potentially resulting in more particle oxidation, modification, and reduction in size, with increased arterial wall uptake. Rosuvastatin enhances the catabolism of apoB-100 in both lbLDL and sdLDL.

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“…The cholesterol mass (mg/dl) of each LDL subfraction and the mean LDL particle size (Å) were determined by this assay. The cholesterol contents of HDL subfractions were also determined electrophoretically by the use of high-resolution 3% polyacrylamide gel tubes and the Lipoprint HDL System as previously described [18]. HDL was divided into 10 subfractions using this assay.…”

Section: Laboratory Examinationsmentioning

confidence: 99%

Relationship between plasma phospholipase A2 concentrations and lipoprotein subfractions in patients with stable coronary artery disease

Zhang

et al. 2015

Clinica Chimica Acta

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Comparability of two different polyacrylamide gel electrophoresis methods for the classification of LDL pattern type

Cited by 24 publications

References 25 publications

Small Dense Low-Density Lipoprotein as Biomarker for Atherosclerotic Diseases

Small Dense Low-Density Lipoprotein as Biomarker for Atherosclerotic Diseases

Metabolism and proteomics of large and small dense LDL in combined hyperlipidemia: effects of rosuvastatin

Relationship between plasma phospholipase A2 concentrations and lipoprotein subfractions in patients with stable coronary artery disease

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