Comparability of Pregnancy Specific Protein-B (PSPB) Concentration in Serum and Urine of Kedah-Kelantan (KK) Cattle

Abstract

This study aimed to investigate the effect of serum and urine PSPB levels based on different biomarkers, such as serum and urine. Serum PSPB levels significantly increased from day 0 to day 164 (P<0.05). However, the PSPB urine concentration through the ELISA test showed higher concentration and reached the sensitivity of the ELISA test from Day 0 until Day 164 after AI. The results of urine biomarkers at Day 0, Day 42, and Day 164 were not significant (P>0.05). Serum PSPB production begins to be established after day 24 of parturition due to embryonic development. Furthermore, the urine PSPB concentration is considered an abundant protein circulating in the animal body and ready to be excreted in the urine. In this study, the ELISA test was unsuitable for detecting pregnancy. However, urine biomarkers can potentially become biomarkers by studying the range of concentrations of PSPB that can be considered pregnancy. In addition, the PSPB protein allows for more prolonged survival in the bovine body and decreases gradually from the 22nd to the 54th day after calving. The results demonstrate that PSPB can detect pregnancy early in pregnancy using biomarkers and evolution. Therefore, this study could be improved by examining PSPB levels of pregnant and non-pregnant cattle.